Transcription factor TCP20 regulates peach bud endodormancy by inhibiting DAM5/DAM6 and interacting with ABF2

Abstract

The dormancy-associated MADS-box (DAM) genes PpDAM5 and PpDAM6 have been shown to play important roles in bud endodormancy; however, their molecular regulatory mechanism in peach is unclear. In this study, by use of yeast one-hybrid screening, we isolated a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR transcription factor, PpTCP20, in the peach cultivar ‘Zhongyou 4’ (Prunus persica var. nectarina). The protein was localized in the nucleus and was capable of forming a homodimer. Electrophoretic mobility shift assays demonstrated that PpTCP20 binds to a GCCCR element in the promoters of PpDAM5 and PpDAM6, and transient dual luciferase experiments showed that PpTCP20 inhibited the expression of PpDAM5 and PpDAM6 as the period of the release of flower bud endodormancy approached. In addition, PpTCP20 interacted with PpABF2 to form heterodimers to regulate bud endodormancy, and the content of abscisic acid decreased with the release of endodormancy. PpTCP20 also inhibited expression of PpABF2 to regulate endodormancy. Taken together, our results suggest that PpTCP20 regulates peach flower bud endodormancy by negatively regulating the expression of PpDAM5 and PpDAM6, and by interacting with PpABF2, thus revealing a novel regulatory mechanism in a perennial deciduous tree.

Structure and Expression of Bud Dormancy-Associated MADS-Box Genes (DAM) in European Plum

“…We also highlighted the site II motif associated with the sequence GCCCA. This element is recognized by TCP transcription factors described as down-regulators of PpeDAM5 and PpeDAM6 expression in peach (Wang et al, 2020). Site II motifs were identified in the intronic regions of all six PdoDAM genes and the promoters of PdoDAM3 and PdoDAM6, closely located to the one reported by the literature.…”

WITHDRAWN: Transcriptome and targeted metabolome reveal the regulation network of Lilium davidii var. unicolor during dormancy release

“…The SOC1 members of the DAM family participate in the dormancy and owering of apricot [44]; AtSOC1 has also been linked to oral organ development and early owering [45]. Recent studies have shown that PpTCP20 negatively regulates the expression of PpDAM5 and PpDAM6 to regulate the internal dormancy of peach blossom buds [46]. By inhibiting ABA biosynthesis, activating CK signal transduction and inducing cyclin expression, GhTCP19 promotes the dormancy release of gladiolus bulbs [47].…”

Distinctive Gene Expression Patterns Define Endodormancy to Ecodormancy Transition in Apricot and Peach

“…Recent studies of DAM genes and their homologs SHORT VEGETATIVE PHASE-like (SVL) genes suggest they are cooperatively regulated by several transcription factors, hormones, and epigenetic factors (da Silveira Falavigna et al, 2018). Wang et al (2019) propose peach TEOSINTE BRANCHED1 transcription factor negatively regulates DAM5 and DAM6 expression, resulting in dormancy release . Leida et al (2012b) reported that histone modifications in DAM6 promoter, the second exon and the second intron are involved in dormancy release (Leida et al, 2012b).…”