Transcription factor PyHY5 binds to the promoters of PyWD40 and PyMYB10 and regulates its expression in red pear ‘Yunhongli No. 1’

Wang, Yuying; Zhang, Xiaodong; Zhao, Yiran; Yang, Jin Ok; He, Yingyun; Li, Guochang; Ma, Weirong; Huang, Xinglong; Su, Jun

doi:10.1016/j.plaphy.2020.07.008

Cited by 50 publications

(33 citation statements)

References 56 publications

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“…thaliana , the expression of PAP1 transcription complexes TT8 , TTG1 and EGL3 decreased under low light conditions, and the accumulation of anthocyanins degraded (Cominelli et al, 2008). Light induction enhanced the expression of MYB10 , WD40 and HY5 , and promoted the accumulation of anthocyanins in red pear (Wang et al, 2020). In Litchi , light can also regulate the expression of WD40 gene (Zhang et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

OsTTG1, a WD40 repeat gene, regulates anthocyanin biosynthesis in rice

et al. 2021

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Anthocyanins play an important role in the growth of plants, and are beneficial to human health. In plants, the MYB-bHLH-WD40 (MBW) complex activates the genes for anthocyanin biosynthesis. However, in rice, the WD40 regulators remain to be conclusively identified. Here, a crucial anthocyanin biosynthesis gene was fine mapped to a 43.4-kb genomic region on chromosome 2, and a WD40 gene OsTTG1 (Oryza sativa TRANSPARENT TESTA GLABRA1) was identified as ideal candidate gene. Subsequently, a homozygous mutant (osttg1) generated by CRISPR/Cas9 showed significantly decreased anthocyanin accumulation in various rice organs. OsTTG1 was highly expressed in various rice tissues after germination, and it was affected by light and temperature. OsTTG1 protein was localized to the nucleus, and can physically interact with Kala4, OsC1, OsDFR and Rc. Furthermore, a total of 59 hub transcription factor genes might affect rice anthocyanin biosynthesis, and LOC_Os01g28680 and LOC_Os02g32430 could have functional redundancy with OsTTG1. Phylogenetic analysis indicated that directional selection has driven the evolutionary divergence of the indica and japonica OsTTG1 alleles. Our results suggest that OsTTG1 is a vital regulator of anthocyanin biosynthesis, and an important gene resource for the genetic engineering of anthocyanin biosynthesis in rice and other plants.

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Section: Discussionmentioning

confidence: 99%

OsTTG1, a WD40 repeat gene, regulates anthocyanin biosynthesis in rice

et al. 2021

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“…Plants respond to abiotic stress by production of protective metabolites. The regulation of anthocyanin biosynthesis by MYB factors is known to be affected by environmental stress or other external signals [103,156]. Genes associated with light stress or other abiotic stress responses (such as Ultraviolet-B light (UV-B) radiation, low temperature or drought) may exert strong effects.…”

Section: Environment Affects Myb Gene Regulation Of Anthocyanin Biosynthesismentioning

confidence: 99%

“…PAP1 expression was highly dependent on the presence of the HY5 TF, and this optical signaling pathway was affected by photosynthesis [24]. In the Asian pear (Pyrus pyrifolia), PyHY5 was also determined to directly recognize and combine with PyMYB10 and PyWD40 to activate anthocyanin biosynthesis [103]. Photosensitivity plays an important role in the regulation of anthocyanins.…”

Section: Lightmentioning

confidence: 99%

“…MYBx repressors inhibit the formation of the MBW complex by binding to bHLH proteins, preventing the activation of the target gene DFR [44]. When the mature flowers and fruits of Petunia are photoinduced, the light signaling pathway activates R2R3-MYBs and MBW (including bHLH1) to form an activation complex, inducing the expression of bHLH [103,106,160]. Consequent bHLH2 forms an MBW complex with the activator and WDR protein and acts on the promoter of the late acting anthocyanin structural gene DFR to regulate the downstream network of anthocyanin biosynthesis [160].…”

Section: Integrated Control Networkmentioning

confidence: 99%

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MYB-Mediated Regulation of Anthocyanin Biosynthesis

Yan

Pei

Zhang

et al. 2021

IJMS

173

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Anthocyanins are natural water-soluble pigments that are important in plants because they endow a variety of colors to vegetative tissues and reproductive plant organs, mainly ranging from red to purple and blue. The colors regulated by anthocyanins give plants different visual effects through different biosynthetic pathways that provide pigmentation for flowers, fruits and seeds to attract pollinators and seed dispersers. The biosynthesis of anthocyanins is genetically determined by structural and regulatory genes. MYB (v-myb avian myeloblastosis viral oncogene homolog) proteins are important transcriptional regulators that play important roles in the regulation of plant secondary metabolism. MYB transcription factors (TFs) occupy a dominant position in the regulatory network of anthocyanin biosynthesis. The TF conserved binding motifs can be combined with other TFs to regulate the enrichment and sedimentation of anthocyanins. In this study, the regulation of anthocyanin biosynthetic mechanisms of MYB-TFs are discussed. The role of the environment in the control of the anthocyanin biosynthesis network is summarized, the complex formation of anthocyanins and the mechanism of environment-induced anthocyanin synthesis are analyzed. Some prospects for MYB-TF to modulate the comprehensive regulation of anthocyanins are put forward, to provide a more relevant basis for further research in this field, and to guide the directed genetic modification of anthocyanins for the improvement of crops for food quality, nutrition and human health.

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“…For RT-PCR, a TaKaRa company SYBR® Premix Ex Taq Kit was used. PCR ampli cation system refers to the method of Wang et al [35]. RT-PCR ampli cation primer sequences were:…”

Section: Rt-pcrmentioning

confidence: 99%

Transcriptome Analysis, CmMYB1 Gene Clone From Cucumis Melo L. And Its Functional Roles Under Salt Stress  

Zhang

Dai³

et al. 2020

Preprint

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MYB TFs plays an important role in plant adaptation to abiotic stress, especially in response to salt stress. Preliminary research found that the stress resistance of the two melon varieties ‘M15’ and ‘baogua’ are quite different. To this end, we compared the transcriptomes of ‘M15’ and ‘baogua’. Transcriptome analysis found that the expression levels of 12 MYB transcription factors were significantly different between the two varieties.CmMYB1 gene was cloned from muskmelon (Cucumis melo L.), and the subcellular localization results showed that CmMYB1 was expressed in the cytoplasm and nucleus. Transform CmMYB1 gene into Arabidopsis thaliana, observe T3 phenotypes during the vegetative and reproductive growth periods.The results showed that the transgenic A. thaliana phenotype did not change significantly compared with the wild type . After treatment with 200 mM sodium chloride solution for 1 h and 3 h, the CmMYB1 expression increased significantly and began to decline after 6 h of salt stress, indicating that it functions in the early response to salt stress. These results provide a theoretical basis for the further study of the biological function of CmMYB1.

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Transcription factor PyHY5 binds to the promoters of PyWD40 and PyMYB10 and regulates its expression in red pear ‘Yunhongli No. 1’

Cited by 50 publications

References 56 publications

OsTTG1, a WD40 repeat gene, regulates anthocyanin biosynthesis in rice

OsTTG1, a WD40 repeat gene, regulates anthocyanin biosynthesis in rice

MYB-Mediated Regulation of Anthocyanin Biosynthesis

Transcriptome Analysis, CmMYB1 Gene Clone From Cucumis Melo L. And Its Functional Roles Under Salt Stress

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Transcription factor PyHY5 binds to the promoters of PyWD40 and PyMYB10 and regulates its expression in red pear ‘Yunhongli No. 1’

Cited by 50 publications

References 56 publications

OsTTG1, a WD40 repeat gene, regulates anthocyanin biosynthesis in rice

OsTTG1, a WD40 repeat gene, regulates anthocyanin biosynthesis in rice

MYB-Mediated Regulation of Anthocyanin Biosynthesis

Transcriptome Analysis, CmMYB1&nbsp;Gene Clone From Cucumis Melo L. And Its Functional Roles Under Salt Stress&nbsp;&nbsp;

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Transcriptome Analysis, CmMYB1 Gene Clone From Cucumis Melo L. And Its Functional Roles Under Salt Stress