<i>OsTTG1</i>, a WD40 repeat gene, regulates anthocyanin biosynthesis in rice

Xiao-ling, Yang; Wang, Junrui; Xia, Xiuzhong; Zhang, Zongqiong; He, Jie; Nong, Baoxuan; TongPing, Luo; Feng, Rui; Wu, Yanyan; Pan, Yinghua; Xiong, Fei; Yu, Zhen; Chen, Can; Guo, Hui; Xu, Zhenhe; Li, Danting; Deng, Gaofeng

doi:10.1111/tpj.15285

Cited by 71 publications

(44 citation statements)

References 87 publications

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“…High expression of anthocyanin pathway genes leads to a high accumulation of anthocyanins [ 4 , 6 ]. Ectopic expression of CHS gene from rice and UDP-GLUCOSYL TRANSFERASE gene from citrus triggers the anthocyanin and proanthocyanidins accumulation in rice and transgenic Arabidopsis , respectively [ 4 , 27 , 28 ]. The Arabidopsis leucoanthocyanidin dioxygenase ( LDOX ) is required for vacuole development and proanthocyanidin synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…In maize, the glutathione S-transferase enzyme (encoded by Bronze2, BZ2) is essential for anthocyanin synthesis and sequestration into vacuoles. In plants, MYB, basic-helix-loop-helix (bHLH), and WD40 proteins (collectively named as transcriptional activation MBW complex) collectively or individually regulate the expression of anthocyanin pathway genes (above mentioned) to accumulate anthocyanins in the plant tissues [ 14 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

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Novel Insights into Anthocyanin Metabolism and Molecular Characterization of Associated Genes in Sugarcane Rinds Using the Metabolome and Transcriptome

Rao

Duan

Yang

et al. 2021

IJMS

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Saccharum officinarum (sugarcane) is the fifth major cultivated crop around the world. Sugarcane rind is a promising source for anthocyanin pigments; however, limited information is available on the anthocyanin and its biosynthesis in sugarcane rinds. In this study, we have quantified 49 compounds including 6 flavonoids and 43 anthocyanins in the rind of 6 sugarcane cultivars by using LCMS/MS approach. Thirty of them were quantified for the first time in sugarcane. The 43 anthocyanins included 10 cyanidin (Cya), 11 pelargonidin (Pel), 9 peonidin (Peo), 5 malvidin (Mal), 4 delphinidin (Del), and 4 petunidin (Pet) metabolites. High contents of Cya derivatives were observed in the rind of YT71/210 (dark purple rind), such as cya-3-O-(6-O-malonyl)-glu 1283.3 µg/g and cya-3-O-glu 482.67 µg/g followed by ROC22 (red rind) 821.3 µg/g and 409 µg/g, respectively, whereas the YT93/159 (green rind) showed a minimum level of these compounds. Among six cultivars, ROC22 rind has high levels of Peo derivatives such as peo-3-O-glu (197 µg/g), peo-3-O-(6-O-malonyl)-glu (69 µg/g) and peo-3-O-(6-O-p-coumaryl)-glu (55.17 µg/g). The gene expression analysis revealed that some genes, including a MYB(t) gene, were highly associated with the color phenotype. Thus, we cloned and overexpressed the gene in Arabidopsis and found the pinkish brown color in the hypocotyl of all transgenic lines compared with the wild type. Hence, we have quantified a wide range of anthocyanins in major sugarcane cultivars, reported many new anthocyanins for the first time, and concluded that Cya and Peo derivatives are the major contributing factor of dissimilar colors in sugarcane. The finding and the verification of a novel MYB gene involved in anthocyanin biosynthesis have demonstrated that our study was very valuable for gene discovery and genetic improvement of sugarcane cultivars to harvest high anthocyanin contents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Novel Insights into Anthocyanin Metabolism and Molecular Characterization of Associated Genes in Sugarcane Rinds Using the Metabolome and Transcriptome

Rao

Duan

Yang

et al. 2021

IJMS

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“…DkMYB14 is a transcriptional activator/repressor that directly represses proanthocyanindin (PA) biosynthesis and promotes PA non-solubilization resulting in non-astringent persimmon [57]. On the other hand, MPK4 mediates MYB1 phosphorylation that results in induced anthocyanin accumulation [58]. It is also known that biosynthesis of anthocyanins is activated by a specific protein complex known as MBW (for MYB-bHLH-WDR), which is highly conserved in most flowering plants [59].…”

Section: Anthocyanin Biosynthesismentioning

confidence: 99%

Anthocyanin-Rich Vegetables for Human Consumption – Focus on Potato, Sweetpotato and Tomato

Mattoo¹,

Dwivedi²,

Dutt³

et al. 2022

Preprint

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Malnutrition, unhealthy diets, and lifestyle changes have become major risk factors for non-communicable diseases while ad-versely impacting economic growth and sustainable development. Anthocyanins, a group of flavonoids that are rich in fruits and vegetables, contribute positively to human health. This review focuses on genetic variation harnessed through crossbreeding and biotechnology-led approaches for developing anthocyanins-rich fruit and vegetable crops. Significant progress has been made in identifying genes involved in anthocyanin biosynthesis in various crops. Thus, the use of genetics has led to the development and release of anthocyanin-rich crop cultivars in Europe and USA. Such a trend is emerging in the developing world. The purple pota-to “Kufri Neelkanth” has been released for cultivation in northern India, and a few colored grain wheat lines, developed through crossbreeding, are being tested for their productivity and adaptation. Although tomato is deficient in anthocyanins, some of its wild relatives are known to accumulate anthocyanins in their sub-epidermal fruit tissue. In Europe, anthocyanin-rich tomato cul-tivar ‘Sun Black’ developed via the introgression of Aft and atv genes has been released. The development of anthocyanin-rich food crops without any significant yield penalty has been due to the use of genetic engineering involving specific transcription factors or gene editing. The anthocyanin-rich food ingredients have the potential of being more nutritious than those devoid of anthocyanins. The inclusion of anthocyanins as a target characteristic in breeding programs can ensure the development of culti-vars to meet the nutritional needs for human consumption, particularly in the developing world.

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“…Moreover, the biorhythm-related CCT family genes in Medicago truncatula responded to NaCl stress [25]. In plants, WD40 proteins were involved in plant development, response to stress and other physiological activities [26,27]. In addition, several WD40 proteins were found to be involved in the ubiquitination of cellular proteins [28].…”

Section: Hpxbcp3 Responded To Nacl Stress In Microalgaementioning

confidence: 99%

The Papain-like Cysteine Protease HpXBCP3 from Haematococcus pluvialis Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae

Liu

Guo

Huang

et al. 2021

IJMS

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The papain-like cysteine proteases (PLCPs), the most important group of cysteine proteases, have been reported to participate in the regulation of growth, senescence, and abiotic stresses in plants. However, the functions of PLCPs and their roles in stress response in microalgae was rarely reported. The responses to different abiotic stresses in Haematococcus pluvialis were often observed, including growth regulation and astaxanthin accumulation. In this study, the cDNA of HpXBCP3 containing 1515 bp open reading frame (ORF) was firstly cloned from H. pluvialis by RT-PCR. The analysis of protein domains and molecular evolution showed that HpXBCP3 was closely related to AtXBCP3 from Arabidopsis. The expression pattern analysis revealed that it significantly responds to NaCl stress in H. pluvialis. Subsequently, transformants expressing HpXBCP3 in Chlamydomonas reinhardtii were obtained and subjected to transcriptomic analysis. Results showed that HpXBCP3 might affect the cell cycle regulation and DNA replication in transgenic Chlamydomonas, resulting in abnormal growth of transformants. Moreover, the expression of HpXBCP3 might increase the sensitivity to NaCl stress by regulating ubiquitin and the expression of WD40 proteins in microalgae. Furthermore, the expression of HpXBCP3 might improve chlorophyll content by up-regulating the expression of NADH-dependent glutamate synthases in C. reinhardtii. This study indicated for the first time that HpXBCP3 was involved in the regulation of cell growth, salt stress response, and chlorophyll synthesis in microalgae. Results in this study might enrich the understanding of PLCPs in microalgae and provide a novel perspective for studying the mechanism of environmental stress responses in H. pluvialis.

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OsTTG1, a WD40 repeat gene, regulates anthocyanin biosynthesis in rice

Cited by 71 publications

References 87 publications

Novel Insights into Anthocyanin Metabolism and Molecular Characterization of Associated Genes in Sugarcane Rinds Using the Metabolome and Transcriptome

Novel Insights into Anthocyanin Metabolism and Molecular Characterization of Associated Genes in Sugarcane Rinds Using the Metabolome and Transcriptome

Anthocyanin-Rich Vegetables for Human Consumption – Focus on Potato, Sweetpotato and Tomato

The Papain-like Cysteine Protease HpXBCP3 from Haematococcus pluvialis Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae

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