Transcription factor NF-E2 is required for platelet formation independent of the actions of thrombopoeitin/MGDF in megakaryocyte development

Shivdasani, Ramesh A.; Rosenblatt, Margery; Zucker‐Franklin, Dorothea; Jackson, Carl W.; Hunt, Pamela S.; Saris, Christiaan J. M.; Orkin, Stuart H.

doi:10.1016/0092-8674(95)90531-6

Cited by 693 publications

(569 citation statements)

References 53 publications

Supporting

Mentioning

538

Contrasting

Unclassified

Order By: Relevance

“…Each heterodimer binding appeared as a doublet (Fig. 5C, lanes 3, 8, and 13), probably due to the presence of two isoforms of p45 (31), which is consistent with our previous result showing a doublet MAREbinding activity composed of p45 and small Maf in megakaryocytes (20).…”

Section: Smallsupporting

confidence: 81%

“…Genetic and biochemical studies have revealed that the small Maf-p45 heterodimer, which is also known as NF-E2, serves as an indispensable transcriptional activator for terminal megakaryocyte differentiation (17,24,29,31). The megakaryocytes of p45-null mutant mice proliferate and differentiate in response to thrombopoietin, without practically any proplatelet generation.…”

mentioning

confidence: 99%

See 1 more Smart Citation

MafG Sumoylation Is Required for Active Transcriptional Repression

Motohashi

Katsuoka

Miyoshi

et al. 2006

Molecular and Cellular Biology

View full text Add to dashboard Cite

A straightforward mechanism for eliciting transcriptional repression would be to simply block the DNA binding site for activators. Such passive repression is often mediated by transcription factors that lack an intrinsic repressor activity. MafG is a bidirectional regulator of transcription, a repressor in its homodimeric state but an activator when heterodimerized with p45. Here, we report that MafG is conjugated to SUMO-2/3 in vivo. To clarify the possible physiological role(s) for sumoylation in regulating MafG activity, we evaluated mutant and wild-type MafG in transgenic mice and cultured cells. Whereas sumoylation-deficient MafG activated p45-dependent transcription normally and did not affect heterodimer activity, repression by the sumoylation-deficient MafG mutant was severely compromised in vivo. Furthermore, the SUMO-dependent repression activity of MafG was sensitive to histone deacetylase inhibition. Thus, repression by MafG is not achieved through simple passive repression by competing for the activator binding site but requires sumoylation, which then mediates transcriptional repression through recruitment of a repressor complex containing histone deacetylase activity.

show abstract

Section: Smallsupporting

confidence: 81%

mentioning

confidence: 99%

MafG Sumoylation Is Required for Active Transcriptional Repression

Motohashi

Katsuoka

Miyoshi

et al. 2006

Molecular and Cellular Biology

View full text Add to dashboard Cite

show abstract

“…The results of a gene knockout experiment recently indicated that p45 is essential for platelet formation (Shivdasani et al, 1995), whereas MafK (the p18 subunit) appears to be dispensable for normal hematopoiesis as well as other developmental programs (Kotkow and Orkin, 1996). Considering the present results, not only MafK but also other small Maf proteins may contribute to the small p18 subunit of NF-E2.…”

Section: Discussionmentioning

confidence: 99%

Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif

et al. 1997

View full text Add to dashboard Cite

The transcription factor NF-E2, a heterodimeric protein complex composed of p45 and small Maf family proteins, is considered crucial for the regulation of erythroid gene expression and platelet formation. To facilitate the characterization of NF-E2 functions in human cells, we isolated cDNAs encoding two members of the small Maf family, MafK and MafG. The human mafK and mafG genes encode proteins of 156 and 162 amino acid residues, respectively, whose deduced amino acid sequences show approximately 95% identity to their respective chicken counterparts. Expression of mafK mRNA is high in heart, skeletal muscle and placenta, whereas mafG mRNA is abundant in skeletal muscle and is moderately expressed in heart and brain. Both are expressed in all hematopoietic cell lines, including those of erythroid and megakaryocytic lineages. In electrophoretic gel mobility shift assays binding to NF-E2 sites was found to depend on formation of homodimers or heterodimers with p45 and p45-related CNC family proteins. The results suggest that the small Maf family proteins function in human cells through interaction with various basic-leucine zipper-type transcription factors.

show abstract

“…Gene targeting has revealed distinct roles for several CNC family members in mammalian gene regulation. Homozygous disruption of p45 NF-E2 gene in mouse results in defective megakaryopoiesis and profound thrombocytopenia, leading to postnatal death (Shivdasani et al, 1995). Nrf-1-null mutant mice are anemic due to a noncell autonomous defect in de®nitive erythropoiesis and die in utero (Chan et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of human B cell-specific transcription factor BACH2 mapped to chromosome 6q15

et al. 2000

View full text Add to dashboard Cite

The transcription factor Bach2, a member of the BTBbasic region leucine zipper (bZip) factor family, binds to a 12-O-tetradecanoylphorbol-13-acetate (TPA)-responsive element and the related Maf-recognition element (MARE) by forming homodimers or heterodimers with Maf-related transcription factors. Bach2 regulates transcription by binding to these elements. To understand the function in hematopoiesis, we isolated a cDNA clone for human Bach2 (BACH2) encoding a protein of 841 amino acid residues with a deduced amino acid sequence having 89.5% identity to mouse homolog. Among human hematopoietic cell lines, BACH2 is expressed abundantly only in some B-lymphocytic cell lines. RT ± PCR analysis of hematopoietic cells revealed that BACH2 mRNA is expressed in primary B-cells. Enforced expression of BACH2 in a human Burkitt cell line, RAJI that does not express endogenous BACH2, resulted in marked reduction of clonogenic activity, indicating that BACH2 possesses an inhibitory e ect on cell proliferation. Bȳ uorescent in situ hybridization, the BACH2 gene was localized to chromosome 6q15. Because deletion of the long arm of chromosome 6 (6q) is one of the commonest chromosomal alterations in human B-cell lymphoma, we examined for the loss of heterozygosity (LOH) of the BACH2 gene in human B-cell non-Hodgkin's lymphomas (NHL). Among 25 informative cases, ®ve (20%) showed LOH. These results indicate that BACH2 plays important roles in regulation of B cell development.

show abstract

Transcription factor NF-E2 is required for platelet formation independent of the actions of thrombopoeitin/MGDF in megakaryocyte development

Cited by 693 publications

References 53 publications

MafG Sumoylation Is Required for Active Transcriptional Repression

MafG Sumoylation Is Required for Active Transcriptional Repression

Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif

Cloning and expression of human B cell-specific transcription factor BACH2 mapped to chromosome 6q15

Contact Info

Product

Resources

About