Cloning and expression of human B cell-specific transcription factor BACH2 mapped to chromosome 6q15

Sasaki, Susumu; Ito, Etsuro; Toki, Tsutomu; Maekawa, Taira; Kanezaki, Rika; Umenai, Takamichi; Muto, Akihiko; Nagai, Hirokazu; Kinoshita, Tomohiro; Yamamoto, Masayuki; Inazawa, Johji; Taketo, Makoto Mark; Nakahata, Tatsutoshi; Igarashi, Kazuhiko; Yokoyama, Masaru

doi:10.1038/sj.onc.1203716

Cited by 64 publications

(69 citation statements)

References 53 publications

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“…Although Bach2 was reported to be expressed specifically in B cells (11,12), we found that Bach2 was expressed in normal T cells at levels comparable with B cells. Peripheral T cells consist of mainly two populations: naive and memory T cells.…”

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confidence: 62%

Bach2 maintains T cells in a naive state by suppressing effector memory-related genes

Tsukumo

Unno

Muto

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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The transcriptional repressor BTB and CNC homology 2 (Bach2) is thought to be mainly expressed in B cells with specific functions such as class switch recombination and somatic hypermutation, but its function in T cells is not known. We found equal Bach2 expression in T cells and analyzed its function using Bach2-deficient (−/−) mice. Although T-cell development was normal, numbers of peripheral naive T cells were decreased, which rapidly produced Th2 cytokines after TCR stimulation. Bach2 −/− naive T cells highly expressed genes related to effector-memory T cells such as CCR4, ST-2 and Blimp-1. Enhanced expression of these genes induced Bach2 −/− naive T cells to migrate toward CCR4-ligand and respond to IL33. Forced expression of Bach2 restored the expression of these genes. Using Chromatin Immunoprecipitation (ChIP)-seq analysis, we identified S100 calcium binding protein a, Heme oxigenase 1, and prolyl hydroxylase 3 as Bach2 direct target genes, which are highly expressed in effector-memory T cells. These findings indicate that Bach2 suppresses effector memory-related genes to maintain the naive T-cell state and regulates generation of effector-memory T cells. Kruppel-like factor 2 (KLF2) is one of those TF, which regulates the expression of the sphingosine-1-phosphate receptor-1 (S1pr1) in SP cells (2). S1pr1 is required for the egress of SP cells from the thymus, and thus KLF2-deficient (−/−) SP cells accumulate in the thymus. KLF2 positively regulates S1pr1 expression through direct binding to its promoter. KLF2 −/− mice also develop an unusual population of innate-like CD8 +

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mentioning

confidence: 62%

Bach2 maintains T cells in a naive state by suppressing effector memory-related genes

Tsukumo

Unno

Muto

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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132

151

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“…[57][58][59] In BL cell lines these deletions are consistently associated with loss of heterozygosity in the 6q25-27 region. 56 Genes possibly affected by 6q abnormalities include BACH2 (encoding for B-lineage specific transcription factor, a putative tumor suppressor gene), on 6q15, 60,61 and BLIMP1 (B-lymphocyte maturation promoting), on 6q21-q22.1. 62 In conclusion, our study identifies distinct secondary cytogenetic abnormalities in BL, some of which correlate with outcome in pediatric or adult patients.…”

Section: Discussionmentioning

confidence: 99%

Secondary chromosomal abnormalities predict outcome in pediatric and adult high‐stage Burkitt lymphoma

Onciu

Schlette

Zhou

et al. 2006

Cancer

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Recent studies suggest that the hypodigms representing the two earliest Australopithecus (Au. anamensis and Au. afarensis) form an ancestor‐descendant lineage. Understanding the details of this possible transition is important comparative evidence for assessing the likelihood of other examples of ancestor‐descendant lineages within the hominin clade. To this end we have analyzed crown and cusp base areas of high resolution replicas of the mandibular molars of Au. anamensis (Allia Bay and Kanapoi sites) and those of Au. afarensis (Hadar, Laetoli, and Maka). We found no statistically significant differences in crown areas between these hypodigms although the mean of M1 crowns was smaller in Au. anamensis, being the smallest of any Australopithecus species sampled to date. Intraspecies comparison of the areas of mesial cusps for each molar type using Wilcoxon signed rank test showed no differences for Au. anamensis. Significant differences were found between the protoconid and metaconid of Au. afarensis M2s and M3s. Furthermore, the area formed by the posterior cusps as a whole relative to the anterior cusps showed significant differences in Au. afarensis M1s and in Au. anamensis M2s but no differences were noted for M3s of either taxon. Developmental information derived from microstructural details in enamel shows that M1 crown formation in Au. anamensis is similar to Pan and shorter than in H. sapiens. Taken together, these data suggests that the overall trend in the Au. anamensis‐Au. afarensis transition may have involved a moderate increase in M1 crown areas with relative expansion of distal cusps. Am J Phys Anthropol , 2012. © 2012 Wiley Periodicals, Inc.

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“…The packaging cells and NIH3T3 cells were cultured in Dulbecco's modified Eagle's medium (Nissui) with 10% fetal bovine serum (JRH BioSciences), 100 units/ml penicillin, and 100 g/ml streptomycin (Invitrogen). Bach2-overexpressing and control Raji clones were described previously (31). The cells were treated with diethyl maleate (DEM) or H 2 O 2 (WAKO-jyunyaku) at the indicated concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…Because Bach2 is expressed specifically in B-cells among hematopoietic cells (29), we were interested in whether Bach2 influences the sensitivity to oxidative stress among the B-lineage cells as well. Previously, we reported the establishment of Raji cell clones that overexpress human BACH2 (31). These clones seemed useful for examining the effects of Bach2 overexpression in B-cells because Raji cells do not express an endogenous BACH2 (31).…”

Section: Bach2-induced Cell Death Of B-lymphoma Cells-mentioning

confidence: 99%

Activation of Maf/AP-1 Repressor Bach2 by Oxidative Stress Promotes Apoptosis and Its Interaction with Promyelocytic Leukemia Nuclear Bodies

Muto¹,

Tashiro²,

Tsuchiya³

et al. 2002

Journal of Biological Chemistry

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The oxidative stress response operates by inducing the expression of genes that counteract the stress. We show here that the oxidative stress-responsive transcription factor Bach2 is a generic inhibitor of gene expression directed by the 12-O-tetradecanoylphorbol-13-acetate response element, the Maf recognition element, and the antioxidant-responsive element. The Bach2-enhanced green fluorescent protein bicistronic retrovirus was used to monitor the fate of Bach2-expressing cells at the single cell level. Bach2 exerted an inhibitory effect on NIH3T3 cell proliferation and caused massive apoptosis upon mild oxidative stress in both NIH3T3 and Raji B-lymphoid cells. Interestingly, Bach1, a highly homologous protein, could not induce cell death, demonstrating the specificity for the apoptosis induction. Although both oxidative stress and leptomycin B, an inhibitor of nuclear export, induce nuclear accumulation of Bach2, the leptomycin B-induced nuclear accumulation of Bach2 was not sufficient to elicit apoptosis. Upon oxidative stress, Bach2 formed nuclear foci that associated with promyelocytic leukemia nuclear bodies. Our results suggest that Bach2 constitutes a cell lineage-specific system that couples oxidative stress and cell death and that inhibition of 12-O-tetradecanoylphorbol-13-acetate response element, the Maf recognition element, and the antioxidant-responsive element upon oxidative stress may be critical determinants for apoptosis.

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Cloning and expression of human B cell-specific transcription factor BACH2 mapped to chromosome 6q15

Cited by 64 publications

References 53 publications

Bach2 maintains T cells in a naive state by suppressing effector memory-related genes

Bach2 maintains T cells in a naive state by suppressing effector memory-related genes

Secondary chromosomal abnormalities predict outcome in pediatric and adult high‐stage Burkitt lymphoma

Activation of Maf/AP-1 Repressor Bach2 by Oxidative Stress Promotes Apoptosis and Its Interaction with Promyelocytic Leukemia Nuclear Bodies

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