Transcription analysis of peloric mutants of Phalaenopsis orchids derived from tissue culture

Chen, Ya Huei; Tsai, Yi Jung; Ji, Huang; Chen, Fure Chyi

doi:10.1038/sj.cr.7290334

Cited by 44 publications

(25 citation statements)

References 55 publications

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“…The PhActin housekeeping gene was used as an internal control (Table 1). The optimal concentrations of target genes and the internal control PhActin were determined according to Chen et al (2005). Genetics and Molecular Research 13 (4): 10690-10703 (2014) Characterization of genes from Phalaenopsis Three replicates of each reaction were performed, and data were calculated according to the 2 -∆∆Ct method (Livak and Schmittgen, 2001 …”

Section: Quantification Of Transcript Levels By Real-time Pcr Assaymentioning

confidence: 99%

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Yw¹,

Yj²,

Fc³

2014

Genet. Mol. Res.

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ABSTRACT. Somatic embryogenesis receptor-like kinase (SERK) genes have been found to be involved in the somatic embryogenesis of several plant species. We identified and characterized 5 PhSERK genes in the Phalaenopsis orchid. The amino acid sequences of PhSERKs and other SERK proteins are highly conserved, with the highest homology observed in the leucine-rich repeat-receptor-like kinase domain. All 5 PhSERKs were expressed in all Phalaenopsis organs examined (root, leaf, shoot apical meristem, and flower), with the strongest expression, particularly for PhSERK1 and 3, in the shoot apical meristem of mature plants. Expression of all PhSERKs was downregulated during early floral bud development and was upregulated gradually until the semiopen flower stage was reached. All 5 PhSERKs were expressed during both seed germination and protocorm-like-body (PLB) development. In germinated seeds, quantitative real-time PCR revealed upregulation of all PhSERKs except PhSERK4 at 1 week and downregulation after 4 weeks. The 5 PhSERKs were differentially expressed in the early stage Characterization of genes from Phalaenopsis of PLB development and maintained substantial levels during PLB formation, with PhSERK1 and 5 upregulated 1 week after culture and PhSERK2, 3, and 4 downregulated over this period. Because physical wounding of PLB stimulates secondary PLB formation, the PhSERK5 expression peak at week 3 coincided with visible and fully developed secondary PLBs. PhSERK5 may be important in PLB induction and subsequent development. Our PhSERK expression analysis revealed that these genes have a broad role during orchid plant development.

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Section: Quantification Of Transcript Levels By Real-time Pcr Assaymentioning

confidence: 99%

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Yw¹,

Yj²,

Fc³

2014

Genet. Mol. Res.

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“…Although a number of expressed sequence tags (ESTs), transcriptomic and smRNA sequences obtained from Phalaenopsis reproductive organs have been deposited in GenBank or OrchidBase (Chen et al, 2005;Tsai et al, 2006;An et al, 2011;Hsiao et al, , 2011, only a few of these genes and smRNAs have been characterized. Examples are ORAP11, ORAP13, CONSTANS-like, and FVE from Phalaenopsis orchids (Doritaenopsis included), which are believed to play important roles in floral transition and regulation of flowering time (Chen et al, 2007;Zhang et al, 2011;Sun et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Identification of warm day and cool night conditions induced flowering-related genes in a Phalaenopsis orchid hybrid by suppression subtractive hybridization

Zhu

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The influence of warm day and cool night conditions on induction of spikes in Phalaenopsis orchids has been studied with respect to photosynthetic efficiency, metabolic cycles and physiology. However, molecular events involved in spike emergence induced by warm day and cool night conditions are not clearly understood. We examined gene expression induced by warm day and cool night conditions in the Phalaenopsis hybrid Fortune Saltzman through suppression subtractive hybridization, which allowed identification of flowering-related genes in warm day and cool night conditions in spikes and leaves at vegetative phase grown under warm daily temperatures. In total, 450 presumably regulated expressed sequence tags (ESTs) were identified and classified into functional categories, including metabolism, development, transcription factor, signal transduction, transportation, cell defense, and stress. Furthermore, database comparisons revealed a notable number of Phalaenopsis hybrid Fortune Saltzman ESTs that matched genes with unknown function. The expression profiles of 24 genes (from different functional categories) have been confirmed by quantitative real-time PCR in induced spikes and juvenile apical leaves. The results of the real-time PCR showed that, compared to the vegetative apical leaves, the transcripts of genes encoding flowering locus T, AP1, AP2, KNOX1, knotted1-like homeobox protein, R2R3-like MYB, adenosine kinase 2, S-adenosylmethionine synthetase, dihydroflavonol 4-reductase, and naringenin 3-dioxygenase accumulated significantly higher levels, and genes encoding FCA, retrotransposon protein Ty3 and C3HC4-type RING finger protein accumulated remarkably lower levels in spikes of early developmental stages. These results suggested that the genes of two expression changing trends may play positive and negative roles in the early floral transition of Phalaenopsis orchids. In conclusion, spikes induced by warm day and cool night conditions were complex in Phalaenopsis orchids; nevertheless, several molecular flowering pathway-related genes were found. The acquired data form the basis for a molecular understanding of spike induction by warm day and cool night conditions in Phalaenopsis orchids.

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“…Transcription factors function as transcriptional activators in the expression of stress-inducible genes (Chen et al 2005). Transcription factor orthologs of the MYB (FF281759), WRKY (FF281744, HO112153) regulated by the SOS (salt overly sensitive) signaling pathway under salt stress conditions (Kamei et al 2003), MADS (FF281737) and helix-loop-helix DNA-binding domain (HO112151) were evidenced as up-regulated in both 15-and 30-day stressed plants.…”

Section: Tdfs Involved In Transcription Activationmentioning

confidence: 98%

“…Recently, this technique has been successfully used for identifying and isolating sugarcane grassy shoot (SCGS) phytoplasma TDFs and differentially expressed genes in sugarcane under phytoplasma infection condition . In another attempt toward isolating chickpea TDFs expressed during root infection by Fusarium oxysporum (Nimbalkar et al 2006) and transcriptomic analysis of tissue culturederived peloric mutants of Phalaenopsis orchids (Chen et al 2005), this method was found to be highly fruitful.…”

Section: Introductionmentioning

confidence: 99%

Physio-biochemical analysis and transcript profiling of Saccharum officinarum L. submitted to salt stress

et al. 2010

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In an attempt to understand the molecular basis of salt-stress response in sugarcane, physio-biochemical assays and cDNA-RAPD-based gene expression studies under high salt (2% NaCl) supply regimes were initiated. The comparative rates of total protein, proline content and lipid peroxidation were found steadily increased, while total chlorophyll content was decreased in leaves of salttreated over untreated sugarcane plants at corresponding increase in soil electrical conductivity. The comparative transcript responses to salt stress were monitored by ribotyping of both treated and untreated sugarcane plants at early growth stage. Among 335 differentially expressed transcript-derived fragments, 156 up-and 85 down-regulated were reamplified and sequenced. They were functionally categorized as metabolism, DNA/RNA/cellular processes, signal transduction/cell rescue/defense, cell wall modifications, transcriptional regulation, transport/trafficking, retroelements and unknown/hypothetical proteins.

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Transcription analysis of peloric mutants of Phalaenopsis orchids derived from tissue culture

Cited by 44 publications

References 55 publications

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Identification of warm day and cool night conditions induced flowering-related genes in a Phalaenopsis orchid hybrid by suppression subtractive hybridization

Physio-biochemical analysis and transcript profiling of Saccharum officinarum L. submitted to salt stress

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