Identification of warm day and cool night conditions induced flowering-related genes in a Phalaenopsis orchid hybrid by suppression subtractive hybridization

Li, D M; Lu, Fu; Zhu, Genfa; Sun, Yujiao; Xu, Yibin; Jiang, Meng; Liu, J W; Wang, Z

doi:10.4238/2014.february.14.7

Cited by 7 publications

(7 citation statements)

References 41 publications

(39 reference statements)

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“…These results suggest that roots might play an important role before flowering transition in Phalaenopsis hybrid Fortune Saltzman and might be involved in the flowering transition and development signaling pathway. Compared with that in the apical leaves under warm daily temperatures, the expression of PhFT was obviously higher under warm day and cool night conditions; such conditions induced 0-3-mm long spikes and gradually reduced during spike development (Li et al, 2014). The mRNA expression of PhFT during flowering was remarkably higher in the flower buds at the different developmental stages (Figure 5), similar to observations for FT in Oncidium Gower Ramsey (OnFT) (Hou and Yang, 2009).…”

Section: Discussionsupporting

confidence: 73%

“…We obtained one full-length cDNA (JK720571, 809 bp) from a suppression subtractive hybridization cDNA library of Phalaenopsis hybrid Fortune Saltzman spikes induced by warm days and cool nights in a previous study (Li et al, 2014). BLASTx search showed that the cDNA sequence was highly homologous to the FT proteins of C. goeringii (accession No.…”

Section: Characterization Of the Cdna Sequence Of Phftmentioning

confidence: 99%

“…In a previous study, we obtained one full-length cDNA [JK720571, 809 bp, containing the 5'-untranslated region (UTR) and 3'-UTR] from a suppression subtractive hybridization cDNA library obtained from the spikes of Phalaenopsis hybrid Fortune Saltzman subjected to warm day and cool night conditions (Li et al, 2014). The cDNA sequence was highly homologous to the FT proteins of Cymbidium goeringii (92%), Cymbidium faberi (91%), and Oncidium Gower Ramsey (90%) by BLASTx search.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and functional analysis of a Flowering locus T homolog gene from a Phalaenopsis orchid

Dm¹,

Fb²,

Gf³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Warm day and cool night conditions significantly induce reproductive spike formation in Phalaenopsis plants; hence, determining the flowering mechanism regulating the reproductive transition is important. Flowering locus T (FT) plays important roles in flowering induction in several plants. To explore spike induction by warm days and cool nights in Phalaenopsis orchids, we isolated the FT (PhFT) from Phalaenopsis hybrid Fortune Saltzman. The cDNA of PhFT was 809-bp long and contained a 531-bp open reading frame encoding a putative protein of 176 amino acids, a 58-bp 5'-untranslated region (UTR), and a 220-bp 3'-UTR. The predicted molecular mass of PhFT was 19.80 kDa, with an isoelectric point of 8.68. The PhFT was predicted to possess the conserved functional regions of the phosphatidylethanolamine-binding protein superfamily. Nucleotide sequence data indicated that PhFT contained 3 introns and 4 exons. Sequence alignment and phylogenetic analyses of PhFT revealed high homology to the FT proteins of Cymbidium goeringii and Oncidium Gower Ramsey. Quantitative realtime polymerase chain reaction analysis indicated that PhFT mRNA Functional characterization of a PhFT gene was expressed in roots, apical leaves, mature leaves, and flowers. In flowers, PhFT was expressed more in developing floral buds than in mature flowers and was predominantly expressed in ovaries and petals. Ectopic expression of PhFT in Arabidopsis ft-1 mutants showed novel early-flowering phenotypes that lost their siliques. Our results indicated that the ectopic expression of PhFT could partially complement the late flowering defect in transgenic Arabidopsis ft-1 mutants. Our findings suggest that PhFT is a putative FT homolog in Phalaenopsis plants that regulates flowering transition.

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Section: Discussionsupporting

confidence: 73%

Section: Characterization Of the Cdna Sequence Of Phftmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and functional analysis of a Flowering locus T homolog gene from a Phalaenopsis orchid

Dm¹,

Fb²,

Gf³

et al. 2014

Genet. Mol. Res.

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“…The result showed that, in addition to FT , LFY , APETALA1 ( AP1 ) and OVEREXPRESSION OF CONSTANS 1 ( SOC1 ) homologs, genes involved in GA biosynthesis were also up-regulated by low ambient temperature. Another expression analysis using Phalaenopsis Fortune Saltzman found that transcripts of KNOX1 , R2R3-like MYB , adenosine kinase 2, S-adenosylmethionine synthetase, dihydroflavonol 4-reductase, and naringenin 3-dioxygenase were accumulated at a higher level in spikes grown under warm day/cool night (28 °C/21 °C) compared with those grown at daily warm temperature (28 °C/26 °C) under natural light conditions (Li et al, 2014).…”

Section: Floral Transition Of Orchidsmentioning

confidence: 99%

“…However, we cannot exclude the possibility that the pavement for the PaFT1 movement to the dormant/juvenile buds is not available. Based on the transcriptomic analyses of Phalaenopsis orchids, KNOX1 , SOC1 and FVE genes are induced by low ambient temperature (Sun et al, 2012; Li et al, 2014; Huang et al, 2016) although Dendrobium KNOX1 gene is down-regulated by low ambient temperature (Yu and Goh, 2000a; Yu et al, 2000). Thus, the role of KNOX1 in floral transition of Phalaenopsis needs to be further examined.…”

Section: Floral Transition Of Orchidsmentioning

confidence: 99%

Floral Induction and Flower Development of Orchids

et al. 2019

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Orchids comprise one of the largest, most highly evolved angiosperm families, and form an extremely peculiar group of plants. Various orchids are available through traditional breeding and micro-propagation since they are valuable as potted plants and/or cut flowers in horticultural markets. The flowering of orchids is generally influenced by environmental signals such as temperature and endogenous developmental programs controlled by genetic factors as is usual in many flowering plant species. The process of floral transition is connected to the flower developmental programs that include floral meristem maintenance and floral organ specification. Thanks to advances in molecular and genetic technologies, the understanding of the molecular mechanisms underlying orchid floral transition and flower developmental processes have been widened, especially in several commercially important orchids such as Phalaenopsis, Dendrobium and Oncidium. In this review, we consolidate recent progress in research on the floral transition and flower development of orchids emphasizing representative genes and genetic networks, and also introduce a few successful cases of manipulation of orchid flowering/flower development through the application of molecular breeding or biotechnology tools.

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