Transcript profiles of stress-related genes in developing white spruce (Picea glauca) somatic embryos cultured with polyethylene glycol

Stasolla, Claudio; Zyl, Leonel van; Egertsdotter, Ulrika; Craig, Deborah; Liu, Wenbin; Sederoff, Ronald R.

doi:10.1016/s0168-9452(03)00228-0

Cited by 7 publications

(2 citation statements)

References 34 publications

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“…El Dawayati et al (2012) demonstrated the morpho-ontogenetic action of PEG on the normal development of somatic embryos of Phoenix dactylifera, suggesting the involvement of PEG in the activation of morphogenetic processes in these cultures. The improved morphogenic responses of callus matured with PEG may be related to the control of SAM activity (Stasolla et al 2003a), specifically in the expression of important genes for SAM development as Fiddlehead, AGM, and Knotted-like and ZWILLE (Moussian et al 1998;Stasolla et al 2003b).…”

Section: Discussionmentioning

confidence: 99%

Morphological analyses and variation in carbohydrate content during the maturation of somatic embryos of Carica papaya

Vale

Reis

Passamani

et al. 2018

Physiol Mol Biol Plants

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Efficient protocols for somatic embryogenesis of papaya ( L.) have great potential for selecting elite hybrid genotypes. Addition of polyethylene glycol (PEG), a nonplasmolyzing osmotic agent, to a maturation medium increases the production of somatic embryos in .. To study the effects of PEG on somatic embryogenesis of ., we analyzed somatic embryo development and carbohydrate profile changes during maturation treatments with PEG (6%) or without PEG (control). PEG treatment (6%) increased the number of normal mature somatic embryos followed by somatic plantlet production. In both control and PEG treatments, pro-embryogenic differentiation to the cotyledonary stage was observed and was significantly higher with PEG treatment. Histomorphological analysis of embryonic cultures with PEG revealed meristematic centers containing small isodiametric cells with dense cytoplasm and evident nuclei. Concomitant with the increase in the differentiation of somatic embryos in PEG cultures, there was an increase in the endogenous content of sucrose and starch, which appears to be related to a rising demand for energy, a key point in the conversion of . somatic embryos. The endogenous carbohydrate profile may be a valuable parameter for developing optimized protocols for the maturation of somatic embryos in papaya.

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Section: Discussionmentioning

confidence: 99%

Morphological analyses and variation in carbohydrate content during the maturation of somatic embryos of Carica papaya

Vale

Reis

Passamani

et al. 2018

Physiol Mol Biol Plants

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show abstract

“…In Picea glauca (Stasolla et al 2003, Belmonte et al 2005 and Aesculus hippocastanum (Troch et al 2009), PEG 8000 was essential for the conversion of somatic embryos to seedlings. However, Márquez-Martín et al (2011) reported that with PEG 8000, the somatic embryo production of Persea americana Mill.…”

Section: Experiments 1 -Polyethylene Glycol (Peg) On 'Golden Thb' Papamentioning

confidence: 99%

The effects of an osmoregulator, carbohydrates and polyol on maturation and germination of ‘Golden THB’ papaya somatic embryos

Chagas

Cipriano

Lopes

et al. 2018

An. Acad. Bras. Ciênc.

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This study evaluated the effect of osmoregulators and carbohydrates on the maturation and germination of somatic embryos of papaya 'Golden THB'. Cotyledon explants from papaya seedlings germinated in vitro on basal MS medium were cultured on somatic embryogenesis induction medium (IM) containing MS salts, myo-inositol, sucrose, agar and p-chlorophenoxyacetic acid. After 50 days, embryogenic calli were transferred onto maturation media (MM) for 45 additional days. For experiment 1, a MS-based medium supplemented with abscisic acid, activated charcoal and concentrations of PEG 6000 (0; 40; 50; 60 and 70 g L-1) was used, whereas for experiment 2 malt extract concentrations (0; 0.1; 0.2; 0.3 and 0.4 g L-1) were assessed. The normal cotyledonary somatic embryos produced in experiment 2 were transferred to the germination medium (GM). The GM consisted of full-strength MS medium, sucrose, agar and was supplemented with myo-inositol at varying concentrations (0; 0.275; 0.55 and 0.825 mM). The PEG concentrations tested impaired the maturation of 'Golden THB' papaya somatic embryos. The MM, supplemented with malt extract at 0.153 g L-1 , promoted the greatest development of normal somatic embryos (18.28 SE calli-1), that is, two cotyledonary leaves produced 36.56 SE calli-1. The supplementation with 0.45 mM myo-inositol provided the highest germination percentage (47.42%) and conversion to emblings.

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Impact of Whole‐Genome and Expressed Sequence Tag Databases on the Study of Plant Secondary Metabolism

Hagel¹,

Page²,

Facchini³

2006

Medicinal Plant Biotechnology

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Transcript profiles of stress-related genes in developing white spruce (Picea glauca) somatic embryos cultured with polyethylene glycol

Cited by 7 publications

References 34 publications

Morphological analyses and variation in carbohydrate content during the maturation of somatic embryos of Carica papaya

Morphological analyses and variation in carbohydrate content during the maturation of somatic embryos of Carica papaya

The effects of an osmoregulator, carbohydrates and polyol on maturation and germination of ‘Golden THB’ papaya somatic embryos

Impact of Whole‐Genome and Expressed Sequence Tag Databases on the Study of Plant Secondary Metabolism

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