Transcript levels of orf288 are associated with the hau cytoplasmic male sterility system and altered nuclear gene expression in Brassica juncea

Heng, Shuangping; Gao, Jie; Wei, Chao; Chen, Fengyi; Li, Xianwen; Wen, Jing; Yi, Bin; Ma, Chaozhi; Tu, Jinxing; Fu, Tingdong; Shen, Jinxiong

doi:10.1093/jxb/erx443

Cited by 36 publications

(22 citation statements)

References 61 publications

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“…The primers used in our study are listed in Supplementary Table S1. The BjActin gene was used as an internal control gene for mRNA expression (Heng et al, 2018).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

et al. 2020

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Brassica juncea is an important dietary vegetable cultivated and consumed in China for its edible stalks and leaves. The purple leaf mustard, which is rich in anthocyanins, is eye-catching and delivers valuable nutrition. However, the molecular mechanism involved in anthocyanin biosynthesis has not been well studied in B. juncea. Here, histological and transcriptome analyses were used to characterize the purple leaf color and gene expression profiles. Free-hand section analysis showed that the anthocyanin was mainly accumulated in the adaxial epidermal leaf cells. The anthocyanin content in the purple leaves was significantly higher than that in the green leaves. To investigate the critical genes and pathways involved in anthocyanin biosynthesis and accumulation, the transcriptome analysis was used to identify the differentially expressed genes (DEGs) between the purple and green leaves from the backcrossed BC3 segregation population in B. juncea. A total of 2,286 different expressed genes were identified between the purple and green leaves. Among them, 1,593 DEGs were up-regulated and 693 DEGs were down-regulated. There were 213 differently expressed transcription factors among them. The MYB and bHLH transcription factors, which may regulate anthocyanin biosynthesis, were up-regulated in the purple leaves. Interestingly, most of the genes involved in plant-pathogen interaction pathway were also up-regulated in the purple leaves. The late biosynthetic genes involved in anthocyanin biosynthesis were highly upregulated in the purple leaves of B. juncea. The up regulation of BjTT8 and BjMYC2 and anthocyanin biosynthetic genes (BjC4H, BjDFR, and BjANS) may activate the purple leaf formation in B. juncea. This study may help to understand the transcriptional regulation of anthocyanin biosynthesis in B. juncea.

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“…The primers used in our study are listed in Supplementary Table S1. The BjActin gene was used as an internal control gene for mRNA expression (Heng et al, 2018).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

et al. 2020

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“…Transcript analysis detected differences in the expression of nuclear genes involved in the development of the anther and, thus, proposed a mechanism of retrograde regulation for orf288 (26).…”

Section: Molecular Mechanisms Responsible For Cmsmentioning

confidence: 97%

“…In fact, there are CMS lines highly used in breeding programs, in which a specific restorer line has been developed, but the identity of the gene responsible of the male sterile condition remains unknown (4,28,40,77,78). To date, several modes of action for CMS genes have been described; namely, energy deficiency, cytotoxic proteins (26), aberrant programmed cell death (58,66) and retrograde signaling from mitochondria that affect nuclear pathways (12). However, the exact relationship between the candidate CMS gene and the observed phenotype has not been assessed in the majority of the cases.…”

Section: Molecular Mechanisms Responsible For Cmsmentioning

confidence: 99%

Male Sterility and Somatic Hybridization in Plant Breeding

García¹,

Edera²,

Marfil³

et al. 2019

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Plant male sterility refers to the failure in the production of fertile pollen. It occurs spontaneously in natural populations and may be caused by genes encoded in the nuclear (genic male sterility; GMS) or mitochondrial (cytoplasmic male sterility; CMS) genomes. This feature has great agronomic value for the production of hybrid seeds and has been widely used in crops, such as corn, rice, wheat, citrus, and several species of the family Solanaceae. Mitochondrial genes determining CMS have been uncovered in a wide range of plant species. The modes of action of CMS have been classified in terms of the effect they produce in the cell, which ultimately leads to a failure in the production of pollen. Male fertility can be restored by nuclear-encoded genes, termed restorer-of-fertility (Rf) factors. CMS from wild plants has been transferred to species of agronomic interest through somatic hybridization. Somatic hybrids have also been produced to generate CMS de novo upon recombination of the mitochondrial genomes of two parental plants or by separating the CMS cytoplasm from the nuclear Rf alleles

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“…Among the different CMS types, ogu [25]and pol [26]CMS systems are widely used in hybrid breeding of vegetableBrassicas.Once developed the CMS phenotype and the associated mitochondrial genes exhibit a maternal inheritance pattern.Numerous CMS related genes (orfs) have been identified and well characterized in different plant species. The key mitochondrial genes (orfs) associated with different CMS typesin Brassicaceae are orf138 correlated with Ogura CMS [21,25], orf125 associated with kosenaCMS of Radish [27], orf72 correlated with murCMS of Diplotaxismuralis [28], orf222 associated with'nap CMS' of Brassica napus [29], orf224/atp6correlated with pol CMS [26,30], orf288 linked with hauCMS of B. juncea [31], orf263 correlated with 'tour CMS' of Brassica tournefortii [32], and orf220 associated with 'tuber mustard CMS' of Brassica juncea [33]. The gene specific primers for these mitochondrial genomic regions can beefficiently utilizedto differentiate different CMS types in Brassica crops.The complete mitochondrial genome sequence of many Brassica species and other important genera belonging to Brassicaceae (e.g.…”

Section: Introductionmentioning

confidence: 99%

Elucidating mitochondrial DNA-markers of Ogura-based CMS linesin Indian cauliflowers (Brassica oleracea var. botrytis L.) and their floral abnormalities due to diversity in cyto-nuclear interaction

Singh

Bhatia

Kumar

et al. 2020

Preprint

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Background: Mitochondrial markers can be used to differentiate diverse mitotypes as well as cytoplasms in angiosperms. In cauliflower, cultivation of hybrids is pivotal in remunerative agriculture and cytoplasmic male sterile lines constitute an important component of the hybrid breeding. Thus, the breeders look for utilizing diverse male sterile cytoplasms in hybrid progamme. In diversifying source of male sterility,it is essential to appropriately differentiate among the available male sterile cytoplasms in cauliflower.PCR polymorphism atthe key mitochondrial genes associated with male sterility will be useful in developing mitochondria specific markers for the different male sterile cytoplasms. Also, the auto and alloplasmic cytonuclear combinations result in complex floral abnormalities.Thus, the study aimed at developing mitotype specific markers of the sterile cytoplasms and to unravel thegenetic effects of the cytonuclear interactions on flower morphology in Indian cauliflowers.Results: In PCR based analysis using a set of primers targeted to orf-138, 76 Indian cauliflower lines showed presence of Ogura cytoplasm though amplicons showed polymorphism within the ofr-138 sequence. The polymorphic loci were found to be spanning over 200-280 bp and 410-470bp genomic regions of BnTR4 and orf125, respectively. Sequence analysis revealed that such cytoplasmic genetic variations could be due to single nucleotide polymorphisms and insertion or deletions of31/51 nucleotides.The cytoplasmic effects on varying nuclear-genetic backgrounds led to varying degree of floral malformations ranging from reduction in flower size, stamens and style length, modification in position of styleand anthers, absence of non-functional stamens to other floral abnormalities. These floral malformations caused dysplasia of flower structure affecting female fertility and inefficient nectar production.Conclusions: The mitochondria specific markers can distinguish ogurabased male sterile cytoplasm. Large number of Indian cauliflower lines showed mitotype variations even within the ogura based cytoplasm. Variable nucleo-cytoplasmic interactions resulted into diverse type of floral malformationsin addition to pollen sterility even within the group of ogura based CMS lines. The finding provide important reference ameliorate understanding of mechanism of cytonuclear interactions in floral organ development in Brassicas. The study will help the breeders in selecting CMS lines without any floral abnormalities in B. oleracea.

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Transcript levels of orf288 are associated with the hau cytoplasmic male sterility system and altered nuclear gene expression in Brassica juncea

Abstract: High transcript levels of the CMS-associated gene orf288 in the hau CMS line of Brassica juncea induce defects in mitochondrial ultrastructure during the development of archesporial cells and cause cytoplasmic male sterility.

Cited by 36 publications

References 61 publications

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

Male Sterility and Somatic Hybridization in Plant Breeding

Elucidating mitochondrial DNA-markers of Ogura-based CMS linesin Indian cauliflowers (Brassica oleracea var. botrytis L.) and their floral abnormalities due to diversity in cyto-nuclear interaction

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