Transcript expression bias of phosphatidylethanolamine binding protein gene in bumblebee, Bombus lantschouensis (Hymenoptera: Apidae)

Dong, Jie; Han, Lei; Wang, Ye; Huang, Jiaxing; Wu, Jie

doi:10.1016/j.gene.2017.06.041

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…Queen bee acid, 10-hydroxydecenoic acid (10-HDA, 1 ), comprises a trans-double bond and a terminal hydroxyl group on a ten-carbon chain and is typically found in Royal Jelly (RJ) with the closely related 10-hydroxydecanoic acid (10-HDAA, 2 ) and sebacic acid ( 3 ), Fig. 4 (Dixon & Shuel, 1978 ; Spannhoff et al, 2011 ; Terada et al, 2011 ). Most studies to date have focused on 1 and 2 , with a considerable body of evidence showing that both are consistently present in RJ wherever sampled (Ferioli et al, 2014 ; Howe et al, 1985 ; Isidorov et al, 2012 ; Lercker et al, 1981 ; Lercker et al, 1982 ; Wang et al, 2016 ; Wytrychowski et al, 2013 ; Xu & Gao, 2013 ; Zheng et al, 2011 ).…”

Section: Sterolsmentioning

confidence: 99%

“…The presence of so much of 1 during the early development of bees has raised questions about the metabolic or developmental function of this compound. There is evidence for activity as a histidine deacetylase inhibitor and TRPA1 activator (Spannhoff et al, 2011 ; Terada et al, 2011 ), and a general role in development (Dixon & Shuel, 1978 ; Kinoshita & Shuel, 1975 ; Šedivá et al, 2018 ). However, the range of effects that 1 and 2 produce or contribute to is unclear, as is how they are controlled.…”

Section: Sterolsmentioning

confidence: 99%

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Sterol and lipid metabolism in bees

Furse,

Koch,

Wright

et al. 2023

Metabolomics

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Background Bees provide essential pollination services for many food crops and are critical in supporting wild plant diversity. However, the dietary landscape of pollen food sources for social and solitary bees has changed because of agricultural intensification and habitat loss. For this reason, understanding the basic nutrient metabolism and meeting the nutritional needs of bees is becoming an urgent requirement for agriculture and conservation. We know that pollen is the principal source of dietary fat and sterols for pollinators, but a precise understanding of what the essential nutrients are and how much is needed is not yet clear. Sterols are key for producing the hormones that control development and may be present in cell membranes, where fatty-acid-containing species are important structural and signalling molecules (phospholipids) or to supply, store and distribute energy (glycerides). Aim of the review In this critical review, we examine the current general understanding of sterol and lipid metabolism of social and solitary bees from a variety of literature sources and discuss implications for bee health. Key scientific concepts of review We found that while eusocial bees are resilient to some dietary variation in sterol supply the scope for this is limited. The evidence of both de novo lipogenesis and a dietary need for particular fatty acids (FAs) shows that FA metabolism in insects is analogous to mammals but with distinct features. Bees rely on their dietary intake for essential sterols and lipids in a way that is dependent upon pollen availability.

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Section: Sterolsmentioning

confidence: 99%

Section: Sterolsmentioning

confidence: 99%

Sterol and lipid metabolism in bees

Furse,

Koch,

Wright

et al. 2023

Metabolomics

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“…With the development of nextgeneration sequencing, the genomes of two bumblebees, B. terrestris and Bombus impatiens , have been completed (Sadd et al 2015), which have greatly promoted the exploration of gene function (Pertea et al 2016;Romero et al 2012). Gene expression characteristics as components of gene function are frequently examined at different developmental stages (Dong et al 2017;Han et al 2018;Li et al 2010). Metamorphosis stages are key points at which master gene regulation mechanisms act during heterogeneous changes (Guan et al 2019;Li et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification of reference genes for gene expression analysis at different developmental stages of the bumblebee Bombus terrestris (Hymenoptera: Apidae)

et al. 2021

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Bumblebees are important pollinators for many wild plants and crops. To explore gene expression characteristics in depth, stable reference genes will be essential across different developmental stages. In this context, we evaluated the stability of eight common reference genes at ten developmental stages in the bumblebee Bombus terrestris (B. terrestris ) with four algorithms and two rank approaches. The results showed that all the primers for the reference genes exhibited high specificity, linear correlation (R 2 -value 0.9917~0.9978) and a good PCR amplification efficiency (E -value 95.4~108.6%). The combined use of RPL13 and RP49 was suggested to be the most suitable reference genes. Our candidate reference genes will help to improve the precision of the detection of gene expression at different developmental stages of bumblebee species. bumblebee / Bombus terrestris / development stage / reference gene / qRT-PCR

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“…In only a few bee species, including A. mellifera and a stingless bee Melipona scutellaris , are keys and images available for pupal stages (Cardoso-Júnior et al, 2017; Daly, 1966; Elias-Neto, Soares & Bitondi, 2009). Thus far, attempts to separate bumble bee pupa by morphology has involved either using a modification of the same six stages for honey bees or a more simplified model (Dong et al, 2017; Li et al, 2010; Mänd et al, 2005). Morphological keys from other species like honey bees are not enough to represent fine increments of pupal development in bumble bees needed for developmental genetic work.…”

Section: Introductionmentioning

confidence: 99%

Morphological characterization and staging of bumble bee pupae

Tian

Hines

2018

PeerJ

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Bumble bees (Hymenoptera: Apidae, Bombus) are important pollinators and models for studying mechanisms underlying developmental plasticity, such as factors influencing size, immunity, and social behaviors. Research on such processes, as well as expanding use of gene-manipulation and gene expression technologies, requires a detailed understanding of how these bees develop. Developmental research often uses time-staging of pupae, however dramatic size differences in these bees can generate variation in developmental timing. To study developmental mechanisms in bumble bees, appropriate staging of developing bees using morphology is necessary. In this study, we describe morphological changes across development in several bumble bee species and use this to establish morphology-based staging criteria, establishing 20 distinct illustrated stages. These criteria, defined largely by eye and cuticle pigmentation patterns, are generalizable across members of the subgenus Pyrobombus, and can be used as a framework for study of other bumble bee subgenera. We examine the effects of temperature, caste, size, and species on pupal development, revealing that pupal duration shifts with each of these factors, confirming the importance of staging pupae based on morphology rather than age and the need for standardizing sampling.

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Transcript expression bias of phosphatidylethanolamine binding protein gene in bumblebee, Bombus lantschouensis (Hymenoptera: Apidae)

Cited by 5 publications

References 38 publications

Sterol and lipid metabolism in bees

Sterol and lipid metabolism in bees

Identification of reference genes for gene expression analysis at different developmental stages of the bumblebee Bombus terrestris (Hymenoptera: Apidae)

Morphological characterization and staging of bumble bee pupae

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