Identification of reference genes for gene expression analysis at different developmental stages of the bumblebee Bombus terrestris (Hymenoptera: Apidae)

Qin, Haoran; Dong, Kun; Huang, Jiaxing; He, Shuhua; Wu, Jie

doi:10.1007/s13592-021-00850-0

Cited by 3 publications

(2 citation statements)

References 50 publications

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“…A preliminary biosafety assessment of the 1895 + 2271 + 2315 combination was performed on three beneficial insects. Bombus terrestris is one of the important pollinating insects in modern agriculture, 40 N. vitripennis is one of the most widely researched model parasitoids, 41 and H. axyridis is used as a natural predator in biological control for crop protection in IPM systems 42 . The biosafety of pesticides for beneficial insects is crucial to the success of an IPM program.…”

Section: Discussionmentioning

confidence: 99%

Insecticidal efficacy and risk assessment of different neuropeptide analog combinations against the peach‐potato aphid following topical exposure

Shi

Nachman

et al. 2022

Pest Management Science

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Background: Insect neuropeptides control essential physiological metabolic activities. In our previous studies, Capability/ CAP2b (PK/CAPA) analog 1895 applied alone or as a combination of CAPA analogs (1895 + 2315) was reported to decrease aphid fitness. While this was obtained with the combination of two peptide analogs of the same neuropeptide class, the effect of combining peptide analogs of different neuropeptide classes has not been explored so far.Results: In this study, we assessed the effect of combinations of the PK/CAPA analog 1895 with neuropeptide analogs of four different classes [adipokinetic hormone (AKH) analog: 2271; myosuppressin analog: 2434; kinin analog: 2460; tachykininrelated peptide analog: 2463] on the fitness of aphids. We found that the combination of 1895 and AKH analog 2271 was the most effective one to control Myzus persicae. The triple combination 1895 + 2271 + 2315 provided a synergistic effect by further increasing aphid mortality and reducing reproduction relative to 1895 + 2315. Additionally, a biosafety assessment of the combination 1895 + 2271 + 2315 showed no significant lethal nor sub-lethal effects on survival rates and food intake for the pollinator (Bombus terrestris) and the two representative natural enemies (Harmonia axyridis and Nasonia vitripennis).Conclusion: These results could facilitate establishment of the triple combination 1895 + 2271 + 2315, and/or inclusion of second generation analogs, as alternatives to broad spectrum and less friendly insecticides.

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Section: Discussionmentioning

confidence: 99%

Insecticidal efficacy and risk assessment of different neuropeptide analog combinations against the peach‐potato aphid following topical exposure

Shi

Nachman

et al. 2022

Pest Management Science

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“…May some insects related to the same family were not varied in the point mutation detected in the gene encoding the enzyme AChE, As results found by Raboudi, et al [49] in the potato aphid, Macrosiphum euphorbiae, and Aphis gossypii resistant to a carbamate found one point mutation produced in the amino acid sequence of Ace1 gene. Generally many searches cited that resistance to OP and py detected in insects showed AChE modification as single or multiple amino acid substitutions as drosophila and others [50].…”

Section: Genotyping Analysis Of Pgossypiella Samplesmentioning

confidence: 99%

Diagnostic expression of the cotton pink bollworm Pectinophora gossypiella (Saunders) Ace1 exposed to some insecticide by the real-time PCR

Diab

2023

irjis

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The Pink Bollworm Pectinophora gossypiella (Saunders) is a devastating pest in Egyptian cotton field. Insecticide control failure may be happen due to its biological features of hide inside bolls and rarely exposed to insecticide application. Thus, a bulk of bolls infested with larvae collected from fields of Sharkia and Benysuef governorate. Moreover, exposed to py, Op sub lethal concentrations in laboratory bioassay. Toxicity features as LC50, LC90 and the slope, and RR was completed. Whereas the common target site of OP and pyrethroid is the acetylcholinesterase enzyme. Thus, the gene Ace1 encoding AChE relative expression was investigated using molecular marker of real-time PCR procedures via the steps of generating data was RNA isolation and characterization, cDNA synthesis, then generating normalization factors, and Ct data attained and analyzed using 2-ΔΔCT method to quantify the gene expression quantitatively of insecticide treated larvae tissue extracted. Data showed the most expressed ratio of samples was cypermethrin followed by deltamethrin compared with the control and the least was malathion and chlorpyrifos, thus, the most effective insecticide was malathion and the least were cypermethrin according to ct values. Hierarchical clustering combined with Heat map and principle component analysis based on various clustering systems and algorithm as distance measures were completed. Validation of reference gene in addition to gene expression stability analyzed by the three optimal gene finders and showed that deltamethrin and cypermethrin treated genes having lower mean weights and considered transcriptionally stable and ideal reference genes.

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Transcriptome‐wide analysis uncovers regulatory elements of the antennal transcriptome repertoire of bumblebee at different life stages

Dikmen,

Dabak,

Özgişi

et al. 2024

Insect Molecular Biology

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Bumblebees are crucial pollinators, providing essential ecosystem services and global food production. The success of pollination services relies on the interaction between sensory organs and the environment. The antenna functions as a versatile multi‐sensory organ, pivotal in mediating chemosensory/olfactory information, and governs adaptive responses to environmental changes. Despite an increasing number of RNA‐sequencing studies on insect antenna, comprehensive antennal transcriptome studies at the different life stages were not elucidated systematically. Here, we quantified the expression profile and dynamics of coding/microRNA genes of larval head and antennal tissues from early‐ and late‐stage pupa to the adult of Bombus terrestris as suitable model organism among pollinators. We further performed Pearson correlation analyses on the gene expression profiles of the antennal transcriptome from larval head tissue to adult stages, exploring both positive and negative expression trends. The positively correlated coding genes were primarily enriched in sensory perception of chemical stimuli, ion transport, transmembrane transport processes and olfactory receptor activity. Negatively correlated genes were mainly enriched in organic substance biosynthesis and regulatory mechanisms underlying larval body patterning and the formation of juvenile antennal structures. As post‐transcriptional regulators, miR‐1000‐5p, miR‐13b‐3p, miR‐263‐5p and miR‐252‐5p showed positive correlations, whereas miR‐315‐5p, miR‐92b‐3p, miR‐137‐3p, miR‐11‐3p and miR‐10‐3p exhibited negative correlations in antennal tissue. Notably, based on the inverse expression relationship, positively and negatively correlated microRNA (miRNA)–mRNA target pairs revealed that differentially expressed miRNAs predictively targeted genes involved in antennal development, shaping antennal structures and regulating antenna‐specific functions. Our data serve as a foundation for understanding stage‐specific antennal transcriptomes and large‐scale comparative analysis of transcriptomes in different insects.

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Identification of reference genes for gene expression analysis at different developmental stages of the bumblebee Bombus terrestris (Hymenoptera: Apidae)

Cited by 3 publications

References 50 publications

Insecticidal efficacy and risk assessment of different neuropeptide analog combinations against the peach‐potato aphid following topical exposure

Insecticidal efficacy and risk assessment of different neuropeptide analog combinations against the peach‐potato aphid following topical exposure

Diagnostic expression of the cotton pink bollworm Pectinophora gossypiella (Saunders) Ace1 exposed to some insecticide by the real-time PCR

Transcriptome‐wide analysis uncovers regulatory elements of the antennal transcriptome repertoire of bumblebee at different life stages

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