Transcervical embryo recovery in Saanen goats

Abstract

In order to study the embryo recovery rate using the transcervical technique in Saanen goats raised in the tropics, 20 donors were submitted to an oestrus synchronisation treatment using intravaginal progestagen sponges for 11 days. On the ninth day of treatment, goats received intramuscular injections of 50 µg cloprostenol and six injections of porcine follicle stimulating hormone (pFSH) at 12-hour intervals. Does in oestrus were mated by Saanen bucks. Donors received an intramuscular injection of 50 µg cloprostenol 24 hours before embryo collection. At this time, the ovaries were observed by laparoscopy to verify the ovulation rate. The embryo recovery was performed six to seven days after oestrus onset by transcervical technique. Goats in which the cervix was not penetrable were submitted to embryo recovery by laparotomy. The recovered embryos were classified according to the International Embryo Transfer Society (IETS) guidelines. Oestrus was observed in 85% of goats. A response to superovulatory treatment was observed in 65% of goats and a mean ovulation rate of 11.5 ± 6.6 was recorded. The cervix was penetrable in 61.5% of goats. In the remaining goats the embryo recovery was performed by laparotomy. The recovery rate was 53.2% and 36.9% using transcervical and surgical methods, respectively. Quanti-qualitative embryo production was similar in both techniques. Embryo recovery by the transcervical technique was shown to be an alternative for collection of embryos in Saanen goats. However, further studies should be done in order to improve both the cervix penetrability and embryo recovery rate.

Pronuclear Embryo Yield in Canindé and Saanen Goats for DNA Microinjection

“…Regarding to the interval between the end of hormonal treatment and the time of oestrous onset, Saanen goats showed early estrus, when compared with Canindé breed. However, in Saanen breed, the time of oestrous onset was similar to others studies using the same hormonal treatment (Baril and Vallet 1990; Lima‐Verde et al. 2003).…”

“…The response relative to the number of goats in estrus is in accordance with the majority of the studies that employed similar treatments for the two breeds (Lima‐Verde et al. 2003; Souza et al.…”

“…A high percentage of superovulating females was observed for both breeds studied; although it was previously established (Lima‐Verde et al. 2003) that a female needed to have a minimum of five ovulations to be considered responsive to the superovulatory treatment.…”

Successful Use of Endoscopy for Transcervical Cannulation Procedures in the Goat

“…; Suyadi and Holz ; Lima‐Verde et al. ) but less information is available on other transcervical procedures in this species. In this study, traversing the cervix was necessary to non‐surgically place an experimental compound into the uterine body of mature female goats as part of a contraception study.…”

Role of Male effect on Reproductive Efficiency of Nulliparous Santa Inês and Morada Nova ewes raised in Different Regions

“…Por isso é importante racionalizar a eficiência reprodutiva dos rebanhos através do desenvolvimento de estraté-gias que sejam de baixo custo e fácil execução [4]. Nesse contexto, o controle dos eventos reprodutivos contribui para maximizar o manejo reprodutivo, a correta indução e sincronização do estro, seja através de fármacos [11,26] ou por métodos naturais, como o efeito macho [1][2][3]5], que são ferramentas para acelerar o melhoramento genético tendo em vista que permitem concentrar as coberturas em curto período de tempo, os partos ocorrem em épocas favoráveis, além de contribuírem para implementação de programas de reprodução assistida, como a inseminação artificial [23,25] e a transferência de embriões [17].…”