Transaminases in cattle and buffalo (Bubalus bubalis) semen in relation to fertility and seminal characteristics during moderate and colder seasons

Khokhar, Baldev Singh; Singh, Manorama; Chaudhary, K.

doi:10.1016/0378-4320(87)90106-0

Cited by 7 publications

(6 citation statements)

References 8 publications

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“…However, the influence of bulls was significant (PCO.01) for ejaculate volume in both the species (Table 1). These observations compared well with the reports of Saxena and Tripathi (1979), Dabas et al (1982) and Khokhar et al (1987) for the semen of the two species. The ejaculate volume had significant positive correlations (p<0.05) only with total sperm count per ejaculate and AKF'activity of seminal plasma among Friesians and negative correlations (P<O.01) with density, sperni count per ml and GOT-GPT activities in Murrahs.…”

Section: Resultssupporting

confidence: 92%

“…Dabas et al (1982), however, did not find significant differences between Murrahs and Jerseymd Dane bulls for their seminal transaminases. Whereas, Khokhar et al (1987) observed significantly greater activities of both GOT and GPT enzymes in exotic than in the Murrah bull semen. Dabas et al (1982), Dube et al (1982) and Dhami et a2 (1990) also recorded significantly higher LDH activity in cattle than in the buffalo semen.…”

mentioning

confidence: 75%

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Comparative Appraisal of Physicomorphological and Enzymatic Attributes of Semen and Their Interrelationships in Ox and Buffalo Bulls

Dhami

Sahni

1994

Journal of Applied Animal Research

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Dhami, A. J. and Sahni, K. L. 1994. Comparative appraisal of physicomorphological and enzymatic attributes of semen and their interrelationships in ox and buffalo bulls. J. Appl. h i m. Res., 5 : 13-20. The seminal attributes, enzymatic profiles and their interrelationships werestudied in 36ejaculates each from 3 HFand 3 Murrah buffalo bulls during winter season. Ejaculate volume, abnormal sperm percent and G. 0. T. values were higher in Friesian bulk semen, whereas, sperm concentration, live sperm percent, AKP, ACP and LDH activities were higher in Murrah bull semen. Sperm density, pH, mass activity, initial motility, GPT activity, freezability and fertility rates were of the same order in the two species. Most of the seminal attributes except ejaculate volume were interrelated in both the species. The semen enzymes were generally not correlated with seminal attributes in exotic bull semen, whereas,

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Section: Resultssupporting

confidence: 92%

mentioning

confidence: 75%

Comparative Appraisal of Physicomorphological and Enzymatic Attributes of Semen and Their Interrelationships in Ox and Buffalo Bulls

Dhami

Sahni

1994

Journal of Applied Animal Research

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“…Most mammals show a seasonal pattern in reproductive activity that is shaped by seasonal changes in their habitats. Biochemical estimates of blood serum are used for semen evaluation since using semen characteristics alone are not completely satisfactory for semen apprasial in the current practice of commercial artificial insemination [3,4,10,11,14,19]. However, the biochemical evaluation of ram blood and its relationship with physical characteristics are still completely unknown.…”

Section: Introductionmentioning

confidence: 99%

“…The concentration of transaminase enzymes (AST and ALT) in semen is a good indicator of semen quality because it measures sperm membrane stability [5]. Furthermore, many studies have correlated the AST level in semen with sperm concentration [10,20]. The present study is concerned with investigating some major biochemical constituents of blood serum and their relationship with hormonal levels and some reproductive parameters in rams during breeding season under Afyon province conditions.…”

Section: Introductionmentioning

confidence: 99%

Relationship Between Some Reproductive Parameters and Biochemical Properties of Blood Serum in Rams

Gündoğan

Yeni

Uçar

et al. 2004

Archives of Andrology

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The objective of the present study was to describe the relationship between some reproductive parameters, hormonal levels, and some biochemical properties of blood serum in rams under the Afyon province conditions. The total protein and globulin were positively (P < 0.01) correlated with sperm motility and sperm concentration in all of rams. Total lipid was negatively (P < 0.05) correlated with sperm motility and concentration in Daglic, and it was positively (P < 0.01) correlated with percentage of abnormal spermatozoa in Chios rams. Total lipid and cholesterol was positively (P < 0.01) correlated with triiodothyronine in all of rams and was negatively (P < 0.05) correlated with testosterone in Daglic rams. Percentage of abnormal spermatozoa were negatively (P < 0.01) correlated with alanine amino transaminase (ALT) level, but were positively (P < 0.01) associated with aspartate amino transaminase (AST) level and AST/ALT ratio in all of rams.

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“…ALT, an enzyme of transaminase group has significant correlation with percentage of motile spermatozoa, sperm concentration and fertility (Khokhar et al, 1987). Significant effect of dilutors on ALT level was reported by Pandey & Singh (2001).…”

Section: Alanine Aminotransferase (Alt)mentioning

confidence: 91%

Effect of dilutors, breed and preservation time on extracellular enzymatic activity of preserved boar semen

Kanshi¹,

Kumar²

2016

JEBAS

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Semen samples were collected from breeds of boars comprising Large White Yorkshire, Tamworth and Cross of Tamworth and Local breed (T & D) breeds. Experimentation was conducted by collecting 216 semen samples from nine boars. After evaluation of neat semen, samples were diluted with Kiev, Modena and Lactose Egg Yolk dilutors (LEY) subsequently preserved at 15ºC in BOD incubator for up to 96 hours. Diluted semen samples were evaluated at different hours of interval starting at 24 hours interval up to 96 hours for extra-cellular enzyme activity of Aspartate Amino Transferase (AST) and Alanine Amino Transferase (ALT). In neat semen significant (P<0.01) effect of breed on extracellular activity of AST and ALT was recorded and it was lower in T&D boar. Mean extracellular level of AST and ALT did not vary significantly in any of these dilutors and breeds during observed hours of preservation. Irrespective of breeds, extracellular levels of AST and ALT were significantly lower in LEY followed by Kiev and Modena dilutors at 24, 48, 72 and 96 hours of preservation. These dilutors varied significantly at 72 and 96 hours duration of preservation among themselves. Whereas at 24 and 48 hours of preservation, they did not vary significantly between Kiev and LEY dilutors. It was found that irrespective of dilutors, AST and ALT level differed significantly between breeds only at 0 hours of preservation. At other hours of preservation difference in levels of AST and ALT was not found significant between breeds.

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Transaminases in cattle and buffalo (Bubalus bubalis) semen in relation to fertility and seminal characteristics during moderate and colder seasons

Cited by 7 publications

References 8 publications

Comparative Appraisal of Physicomorphological and Enzymatic Attributes of Semen and Their Interrelationships in Ox and Buffalo Bulls

Comparative Appraisal of Physicomorphological and Enzymatic Attributes of Semen and Their Interrelationships in Ox and Buffalo Bulls

Relationship Between Some Reproductive Parameters and Biochemical Properties of Blood Serum in Rams

Effect of dilutors, breed and preservation time on extracellular enzymatic activity of preserved boar semen

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