Effect of dilutors, breed and preservation time on extracellular enzymatic activity of preserved boar semen

Abstract

Semen samples were collected from breeds of boars comprising Large White Yorkshire, Tamworth and Cross of Tamworth and Local breed (T & D) breeds. Experimentation was conducted by collecting 216 semen samples from nine boars. After evaluation of neat semen, samples were diluted with Kiev, Modena and Lactose Egg Yolk dilutors (LEY) subsequently preserved at 15ºC in BOD incubator for up to 96 hours. Diluted semen samples were evaluated at different hours of interval starting at 24 hours interval up to 96 hours for extra-cellular enzyme activity of Aspartate Amino Transferase (AST) and Alanine Amino Transferase (ALT). In neat semen significant (P<0.01) effect of breed on extracellular activity of AST and ALT was recorded and it was lower in T&D boar. Mean extracellular level of AST and ALT did not vary significantly in any of these dilutors and breeds during observed hours of preservation. Irrespective of breeds, extracellular levels of AST and ALT were significantly lower in LEY followed by Kiev and Modena dilutors at 24, 48, 72 and 96 hours of preservation. These dilutors varied significantly at 72 and 96 hours duration of preservation among themselves. Whereas at 24 and 48 hours of preservation, they did not vary significantly between Kiev and LEY dilutors. It was found that irrespective of dilutors, AST and ALT level differed significantly between breeds only at 0 hours of preservation. At other hours of preservation difference in levels of AST and ALT was not found significant between breeds.