Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts

Abstract

Abstract. Ultraviolet (UV) irradiation induces the expression of matrix metalloproteinases (MMPs), disturbing the metabolism of extracellular matrix (ECM), and causes the characteristic changes of photoaging in skin. Inhibition of induction of MMPs is suggested to alleviate photoaging induced by UV irradiation. Zeatin, purified from Zea mays, is a member of the cytokinin group of plant growth factors, the activity of which is attributed to its more stable trans form. In this study, we investigated the effect of trans-Zeatin on UVBinduced matrix metalloproteinase-1 (MMP-1) expression in cultured human skin fibroblasts (HSFs) and studied the mechanisms of its actions. We found that pretreatment with trans-Zeatin significantly inhibits UVB-induced MMP-1 expression and c-Jun activation in a dose-dependent manner. We also observed that trans-Zeatin inhibits UVB-induced phosphorylation of ERK, JNK and p38 MAP kinases (MAPKs) dose-dependently. As expected, PD98059, an ERK inhibitor, SP600125, a JNK inhibitor and SB203580, a p38 MAPK inhibitor effectively inhibit UVB-induced phosphorylation of ERK, JNK and p38 MAPKs, respectively. Moreover, the inhibitory mechanism of trans-Zeatin was further demonstrated in MMP-1 secretion using MAPK-specific inhibitors. PD98059, SP600125 and SB203580 suppressed UVB-induced MMP-1 secretion, which is consistent with the above results. Collectively, our results suggest that trans-Zeatin inhibits UVB-induced MMP-1 expression, which may be mediated by inhibition of ERK, JNK and p38 MAPKs signaling pathways in HSFs. Trans-Zeatin is a potential agent for the management of skin photoaging.

Skin-Derived Precursors against UVB-Induced Apoptosis via Bcl-2 and Nrf2 Upregulation

“…The dose of UVB was based on previous dermal FBs data [24] and the condition was optimized. Briefly, SKPs were cultured in a proliferation medium at 37°C and 5% CO 2 .…”

Molecular mechanism of the anti-inflammatory and skin protective effects of Syzygium formosum in human skin keratinocytes

“…In photoaging skin, changes to the composition of the ECM may occur due to high expression of MMPs (Anggakusuma and Hwang, 2010 ). Signaling molecules such as ERK, JNK and p38 MAPK play an essential role in transmitting extracellular signals into the nucleus to regulate gene expression (Yang et al, 2009 ). The family of MMPs, secreted by fibroblasts and keratinocytes, includes MMP-1, MMP-3 and MMP-9, and they play a critical role in the process of skin photoaging.…”

Shikimic acid protects skin cells from UV-induced senescence through activation of the NAD+-dependent deacetylase SIRT1

“…Protein levels were analyzed as previously described [ 81 ]. Cells were directly lysed in 1x Laemmli Buffer (Bio-Rad, USA), incubated at 95° C and centrifuged.…”

“…Cells were directly lysed in 1x Laemmli Buffer (Bio-Rad, USA), incubated at 95° C and centrifuged. Supernatant was subjected to SDS-polyacrylamide gel electrophoresis, and successive steps were performed as described [ 81 ]. The list of antibodies used is included in Supplementary Table 2 .…”