Anatomy skills are considered essential in all aspects of medical practice, and more so now than before with increasing use of diagnostic imaging techniques and advanced surgery. In conjunction with the revision of the medical study curriculum at the University of Oslo in 2014, we investigated Norwegian physicians’ assessment of their own anatomy knowledge and the educational provision of anatomical skills during and after specialization. A total of 902 Norwegian physicians divided into the specialties of general medicine, internal medicine, surgery, neurology and radiology responded to an anonymous survey. As many as 73% of the physicians had at some time point experienced insufficient anatomy knowledge in their own practice, most commonly among surgeons and neurologists. The respondents expressed a need for supplementary educational provision in anatomy during and after specialization, and 36% were unfamiliar with such offers. Notably, dissection courses seemed unavailable during specialization. Our findings demonstrate a requirement for an improved supply of anatomy education during and after specialization. Anatomy courses that combine dissection, radiological images and clinical application would seem desirable.