TRAIL encapsulated to polypeptide-crosslinked nanogel exhibits increased anti-inflammatory activities in Klebsiella pneumoniae-induced sepsis treatment

Chen, Yu Fon; Chen, Guan Yu; Chang, Chien Hsiang; Su, Yu; Chen, Yi Cheng; Yi, Jing; Jan, Jeng‐Shiung

doi:10.1016/j.msec.2019.04.023

Cited by 28 publications

(17 citation statements)

References 37 publications

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“…Due to the ability to mimic animal tissues, their applications in biomedical fields have been rapidly increasing for several decades [ 4 , 5 , 6 , 7 ]. This has led to a risen request for well-defined hydrogelators with adaptable properties for biomedical applications such as advanced wound healing, drug carriers, tissue engineering scaffolds, and investigating biomechanical functions [ 8 , 9 , 10 , 11 ]. Moreover, hydrogels have exhibited numerous characteristics such as adjustability, pellucidity, and porosity/permeability.…”

Section: Introductionmentioning

confidence: 99%

“…The influence of the balance between hydrophilic and hydrophobic interactions on polypeptide self-assembled morphology has been actively studied. The effect of specific interactions and secondary conformations on block copolypeptides can also be a factor governing polypeptide self-assembly [ 11 , 12 , 16 , 19 , 26 , 27 , 28 ]. Herein, we report the synthesis and hydrogelation of star-shaped diblock copolypeptides based on hydrophilic, coil poly( L -lysine) ( s -PLL) blocked with a hydrophobic, sheet-like polypeptide segment, anticipating the hydrogelation.…”

Section: Introductionmentioning

confidence: 99%

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Polypeptide Composition and Topology Affect Hydrogelation of Star-Shaped Poly(L-lysine)-Based Amphiphilic Copolypeptides

Phan

Huang

Tsai

et al. 2021

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In this research, we studied the effect of polypeptide composition and topology on the hydrogelation of star-shaped block copolypeptides based on hydrophilic, coil poly(L-lysine)20 (s-PLL20) tethered with a hydrophobic, sheet-like polypeptide segment, which is poly(L-phenylalanine) (PPhe), poly(L-leucine) (PLeu), poly(L-valine) (PVal) or poly(L-alanine) (PAla) with a degree of polymerization (DP) about 5. We found that the PPhe, PLeu, and PVal segments are good hydrogelators to promote hydrogelation. The hydrogelation and hydrogel mechanical properties depend on the arm number and hydrophobic polypeptide segment, which are dictated by the amphiphilic balance between polypeptide blocks and the hydrophobic interactions/hydrogen bonding exerted by the hydrophobic polypeptide segment. The star-shaped topology could facilitate their hydrogelation due to the branching chains serving as multiple interacting depots between hydrophobic polypeptide segments. The 6-armed diblock copolypeptides have better hydrogelation ability than 3-armed ones and s-PLL-b-PPhe exhibits better hydrogelation ability than s-PLL-b-PVal and s-PLL-b-PLeu due to the additional cation–π and π–π interactions. This study highlights that polypeptide composition and topology could be additional parameters to manipulate polypeptide hydrogelation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polypeptide Composition and Topology Affect Hydrogelation of Star-Shaped Poly(L-lysine)-Based Amphiphilic Copolypeptides

Phan

Huang

Tsai

et al. 2021

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“…Thus, inflammatory response may be strongly activated in LPS-stimulated vascular tissues, leading to the release of several inflammatory factors that contribute to a cytokine storm. 15 The most common feature of cardiovascular dysfunction in patients with sepsis is arterial hypotension, mainly contributed.…”

Section: Discussionmentioning

confidence: 99%

Identification of circRNA and mRNA expression profiles and functional networks of vascular tissue in lipopolysaccharide‐induced sepsis

Nie

Han

Shen

et al. 2020

J Cellular Molecular Medi

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Sepsis is the most common cause of death in intensive care units. This study investigated the circular RNA (circRNA) and mRNA expression profiles and functional networks of the aortic tissue in sepsis. We established a lipopolysaccharide (LPS)‐induced rat sepsis model. High‐throughput sequencing was performed on the aorta tissue to identify differentially expressed (DE) circRNAs and mRNAs, which were validated by real‐time quantitative polymerase chain reaction (RT‐qPCR). Bioinformatic analysis was carried out and coding and non‐coding co‐expression (CNC) and competing endogenous RNA (ceRNA) regulatory networks were constructed to investigate the mechanisms. In total, 373 up‐regulated and 428 down‐regulated circRNAs and 2063 up‐regulated and 2903 down‐regulated mRNAs were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of mRNAs showed that the down‐regulated genes were mainly enriched in the process of energy generation. CNC and ceRNA regulatory networks were constructed with seven DE circRNAs. The results of functional enrichment analysis of CNC target genes revealed the important role of circRNAs in inflammatory response. The ceRNA network also highlighted the significant enrichment in calcium signalling pathway. Significant alterations in circRNAs and mRNAs were observed in the aortic tissue of septic rats. In addition, CNC and ceRNA networks were established.

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“…To reduce the applied dosage of biocidal agents and enhance the bioavailability at the same time, the conventional biocidal agents can be conjugated or encapsulated into nanogels and enriched in the infected zone, and released to kill bacteria. The widely used biocidal agents in literature include but are not limited to antibiotics [ [161] , [162] , [163] , [164] , [165] , [166] , [167] ], bioactive antimicrobial agents [ [168] , [169] , [170] , [171] , [172] ], silver and gold nanoparticles [ [173] , [174] , [175] , [176] , [177] , [178] , [179] , [180] , [181] , [182] ], and antimicrobial peptides [ [183] , [184] , [185] , [186] , [187] ].…”

Section: Nanogel-based Antimicrobial Systemsmentioning

confidence: 99%

“…However, some undesirable characteristics, such as high toxicity, easy degradation, and low bioavailability by binding to serum proteins, mucins, and other anionic components, have impaired the clinical translation of AMPs [ 240 , 241 ]. Therefore, recently increased attention has been placed on nanogels as carriers for the delivery of AMPs to improve the antimicrobial performance [ [243] , [244] , [245] , [246] , [247] , [248] , [249] , [250] ]. The Malmsten group investigated cationic AMPs incorporated into anionic poly (ethyl acrylate- co -methacrylic acid) (MAA) nanogels in different ways that influenced the encapsulation efficiency and release of AMPs, and the membrane interactions and antimicrobial effects of those nanogels.…”

Section: Nanogel-based Antimicrobial Systemsmentioning

confidence: 99%

Nanogels: A novel approach in antimicrobial delivery systems and antimicrobial coatings

Keskin

Forson

et al. 2021

Bioactive Materials

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The implementation of nanotechnology to develop efficient antimicrobial systems has a significant impact on the prospects of the biomedical field. Nanogels are soft polymeric particles with an internally cross-linked structure, which behave as hydrogels and can be reversibly hydrated/dehydrated (swollen/shrunken) by the dispersing solvent and external stimuli. Their excellent properties, such as biocompatibility, colloidal stability, high water content, desirable mechanical properties, tunable chemical functionalities, and interior gel-like network for the incorporation of biomolecules, make them fascinating in the field of biological/biomedical applications. In this review, various approaches will be discussed and compared to the newly developed nanogel technology in terms of efficiency and applicability for determining their potential role in combating infections in the biomedical area including implant-associated infections.

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TRAIL encapsulated to polypeptide-crosslinked nanogel exhibits increased anti-inflammatory activities in Klebsiella pneumoniae-induced sepsis treatment

Cited by 28 publications

References 37 publications

Polypeptide Composition and Topology Affect Hydrogelation of Star-Shaped Poly(L-lysine)-Based Amphiphilic Copolypeptides

Polypeptide Composition and Topology Affect Hydrogelation of Star-Shaped Poly(L-lysine)-Based Amphiphilic Copolypeptides

Identification of circRNA and mRNA expression profiles and functional networks of vascular tissue in lipopolysaccharide‐induced sepsis

Nanogels: A novel approach in antimicrobial delivery systems and antimicrobial coatings

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