Additionally, they were discovered on the slopes of the Ajlun and Tafela Mountains, as well as in the Irano-Turanian region, which encompasses the Saharo-Arabian and Sudanian regions. 4 Euphorbia plants are generally characterized by their poisonous white, milky latex. 5 Previous reports have shown that latex-bearing plants have been used to treat a wide range of ailments, including diabetes, asthma, dysentery, diarrhea, malaria, and skin conditions. The leaves of these plants have also been eaten to treat constipation and applied externally to treat skin abnormalities like warts. 6,7 It was recognized that a potential reason for the high cancer rates in northern Iran was attributed to grain harvests containing Euphorbia seeds. 8 For hundreds of years, a number of Euphorbia species were utilized in traditional medicine on the premise that they were excellent anticancer medicines. Because of their cytotoxicity, antiviral, antibacterial, and antifungal properties, they were also used to treat warts, tumors, migraines, skin conditions, gonorrhea, and intestinal parasites. 9 Species belonging to this genus have been the subject of various phytochemical investigations that have identified a number of groups of secondary metabolites, including phosphatidyl choline, phosphatidyl triterpene, flavonoids, astriacylglycerols, and diterpenoids. [10][11][12][13][14][15][16][17][18][19] The current study was designed to reveal the main groups of secondary metabolites, in addition to evaluating the antioxidant activity, and cytotoxic potentials of four Euphorbia species from the Jordanian flora including Euphorbia petiolata Banks & Sol., Euphorbia hierosolymitana Boiss. Ex Boiss.var. hierosolymitana,