Tradescantia micronucleus bioassay

Ma, Te­-Hsiu; Cabrera, Guillermo; Chen, R.; Gill, B. S.; Sandhu, Shahbeg S.; Vandenberg, A.L.; Salamone, Michael F.

doi:10.1016/0027-5107(94)90115-5

Cited by 164 publications

(99 citation statements)

References 24 publications

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“…The Tradescantia/micronucleus test (TRAD/MCN test) was performed using Tradescantia clone #4430, a hybrid between T. hirsutiflora and T. subacaulis [Ma et al, 1994]. Young Tradescantia inflorescences were immersed directly in the water in the four basins for 6 hr.…”

Section: Tradescantia/micronucleus (Trad/mcn) Testmentioning

confidence: 99%

Genotoxicity of surface water treated with different disinfectants using in situ plant tests

Monarca

Rizzoni

Gustavino

et al. 2003

Environ and Mol Mutagen

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Disinfection of surface drinking water, in particular water chlorination, results in many by-products with potential genotoxic and/or carcinogenic activity. In the present study, we evaluated the genotoxicity of surface water after treatment with different disinfectants by means of in situ plant genotoxicity assays (micronucleus and chromosomal aberration tests) which can detect both clastogenic and aneugenic effects. The study was carried out at a pilot plant using lake water after sedimentation and filtration. This water supplied four stainless steel basins: three basins were disinfected with sodium hypochlorite, chlorine dioxide, and peracetic acid and the fourth basin containing untreated lake water was used as a control. Plants were exposed in situ in the basins. The study was carried out using water collected in different seasons over a period of about 1 year in order to assess the treatments in different physical and chemical lake water conditions. The micronucleus test in root cells of Vicia faba (Vicia faba/MCN test) revealed genotoxicity in many samples of disinfected water. The micronucleus test in Tradescantia pollen cells and the chromosome aberration test in root cells of Allium cepa showed genotoxic effects only in some disinfected samples, but also revealed genotoxicity in raw water. The results of the study indicated that the Vicia faba/ MCN test was the most sensitive plant assay for disinfected water and that peracetic acid disinfection produced similar or lower genotoxicity than sodium hypochlorite or chlorine dioxide treatment. INTRODUCTIONDrinking water disinfection may produce toxic compounds, particularly if the water is obtained from surface sources. Water chlorination results in mutagenic/carcinogenic by-products derived from the reaction of chlorine with organic compounds (humic and fulvic acids) naturally present in water [Rook, 1974; WHO, 1996]. For this reason it is very important to test alternative disinfectants to chlorine in order to reduce these potential health risks. Among the new disinfectants, peracetic acid (CH 3 -CO-OOH, PAA) deserves to be studied for its application in drinking water disinfection, since it is a potent antimicrobial agent and has many applications in hospitals, laboratories, and factories [Baldry et al., 1991[Baldry et al., , 1995Lefevre et al., 1992]. More recently, PAA has been found to be an effective biocidal compound for wastewater disinfection, and previous research showed that disinfection of lake and river drinking water with PAA gave rise to a very low level of genotoxicity and produced only carboxylic acids [Monarca et al., 2002a], which are not recognized as mutagenic.Drinking water has been intensively examined for gene mutations, primarily by assaying extracts of water in bacterial tests, and several studies on different disinfectants have been carried out [Koivusalo et al., 1994;Loper et al., 1980;Monarca 1985Monarca , 1998Monarca , 2002aRomero et al., 1992;Tuomisto et al., 1990;Wilcox and Williamson, 1986;Wilcox et al., 1988]. Plant genot...

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Section: Tradescantia/micronucleus (Trad/mcn) Testmentioning

confidence: 99%

Genotoxicity of surface water treated with different disinfectants using in situ plant tests

Monarca

Rizzoni

Gustavino

et al. 2003

Environ and Mol Mutagen

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“…Before the first collection, the plants underwent a one-month adaptation period at the two test sites. For each test site 30 young inflorescences were collected monthly and fixed in 45% acetic acid solution and 70% ethyl alcohol (1:3 v/v) for 48 h, and then preserved in 100% ethyl alcohol, according to an adaptation of the protocol established by Ma et al (1994). The inflorescence samplings were done at each site from August 2008 to September 2009.…”

Section: Tradescantia Micronucleus Test (Trad-mcn)mentioning

confidence: 99%

Cashew nut roasting: Chemical characterization of particulate matter and genotocixity analysis

Galvão

Cabral

André

et al. 2014

Environmental Research

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“…Frequencies obtained for negative controls remained below the background rate of up to 2.0 MCN/100 tetrads considered by Pereira et al (2013) as the result of spontaneous mutations that can occur naturally in the species, even when plants are grown in environments free from interference of any type of pollutant. In order to minimize any environmental interference before exposure, it is important that plants be allowed to adapt in controlled conditions (Ma et al, 1994;Klumpp et al, 2004). This procedure was employed in this study, following Cassanego et al (2014).…”

Section: Discussionmentioning

confidence: 99%

Biomonitoring the genotoxic potential of the air on Tradescantia pallida var. purpurea under climatic conditions in the Sinos River basin, Rio Grande do Sul, Brazil

et al. 2015

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The present study evaluated the genotoxic effects of the atmospheric air on Tradescantia pallida var. purpurea in urban areas with different intensities of vehicular traffic and in riparian forest fragments in the Sinos River Basin (Rio Grande do Sul, Brazil), considering the influence of climatic conditions prevailing in these environments. Bimonthly, from May 2012 to March 2013, cuttings with flower buds were exposed for 8 h in urban and riparian forest environments in the municipalities of Caraá, Taquara and Campo Bom in the upper, middle and lower sections, respectively, of the Sinos River Basin. Simultaneously, negative controls were made and climatic data were recorded. Micronuclei (MCN) frequencies were determined in young tetrads of pollen mother cells and expressed as MCN/100 tetrads. Significantly higher MCN frequencies were observed in buds exposed in urban and riparian forest environments in Taquara (up to 7.23 and 4.80, respectively) and Campo Bom (up to 4.90 and 4.23, respectively) than in buds exposed in Caraá (up to 2.90 and 2.50, respectively), in the majority of samplings, and in relation to the negative control (up to 1.93) in all months. Over the course of the period monitored, there were significant variations in MCN frequencies at all sampling points, with the exception of the urban environment in Caraá. For the urban environments, relation between the MCN frequency, vehicular traffic and mean temperature was observed. For the riparian forest fragments, there was no association between MCN frequency and climatic factors. Tradescantia pallida var. purpurea can be considered a useful tool to point out areas with increased atmospheric pollution, since the exposure of plants under severe climatic conditions is avoided to minimize their negative influence on the formation of micronuclei.Keywords: abiotic factors, vehicular emissions, bioindicator, environmental quality, micronucleus. Biomonitoramento do potencial genotóxico do ar em

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Tradescantia micronucleus bioassay

Cited by 164 publications

References 24 publications

Genotoxicity of surface water treated with different disinfectants using in situ plant tests

Genotoxicity of surface water treated with different disinfectants using in situ plant tests

Cashew nut roasting: Chemical characterization of particulate matter and genotocixity analysis

Biomonitoring the genotoxic potential of the air on Tradescantia pallida var. purpurea under climatic conditions in the Sinos River basin, Rio Grande do Sul, Brazil

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