Tradescantia as a biomonitor for pesticide genotoxicity evaluation of iprodione, carbaryl, dimethoate and 4,4′-DDE

Fadic, Ximena; Placencia, Fabián; Domínguez, Ana María Peralta; Cereceda-Balic, Francisco

doi:10.1016/j.scitotenv.2016.09.198

Cited by 20 publications

(6 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Carbaryl (CBR), 1-naphthyl methylcarbamate, is a broadly used insecticide in agricultural fields to control a wide spectrum of insects and pests and acts as a growth regulator in modern agriculture. The United States Environmental Protection Agency (EPA) accordingly reported the maximum acceptable concentration (MAC) of CBR in drinking water and its produce as 0.09 μg/mL. − Correspondingly, Cu 2+ is an essential metal ion for human life as a catalytic and/or cofactor for enzymes, cellular metabolism, oxygen transport, redox reactions, and other processes. Abnormally high Cu 2+ concentrations have been related to several diseases such as neurodegenerative disorders and Alzheimer’s, Parkinson’s, and prion diseases .…”

mentioning

confidence: 99%

A Simple Counting-Based Measurement for Paper Analytical Devices and Their Application

et al. 2022

View full text Add to dashboard Cite

This work introduces the concept of a counting-based measurement on paper analytical devices (cPADs) to improve the utilization of numerous reactions. The design of cPADs consists of two layers of paper substrates; the first layer contains a central sample zone combined with a radial surrounded by 12 detection zones that are predeposited with the various reagents, and the second layer acts as a connection channel between the sample zone and each detection zone. The solution can vertically flow from the first to the second layer and then move through the area to each subsequent detection zone. The analyte level can be evaluated by counting the number of detection zones that change color from a blank signal. Furthermore, our cPADs exhibit a capability of implementation for a broad series of reactions. Compared to the dPAD technique, some reactions that are possibly difficult to apply in such devices can be wholly enabled in our devices. The final color reaction on cPADs can apparently occur due to its identity. We applied this technique to the monitoring of carbaryl (CBR) and copper ions (Cu2+) using different reactions, including azo-coupling and complexation, respectively. Accordingly, this indicates an excellent result validated using the more traditional methods. Our cPADs can be applied for rapid screening of both CBR and Cu2+ in water samples with outstanding accuracy and precision using a naked-eye measurement by a relatively unskilled person. We offer a simple platform on PADs for rapid screening, combining high cost-effectiveness within a miniaturized platform designed for use with onsite applications, which is thus suitable for several different reactions.

show abstract

mentioning

confidence: 99%

A Simple Counting-Based Measurement for Paper Analytical Devices and Their Application

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Some studies carried out using Crepis capillaries show that the IP induces lagging chromosomes and this effect is compatible with the abnormal formation of mitotic spindles . By contrast, the MNi test in Tradescantia pollen tetrads showed negative results . The literature about IP genotoxicity in human cells is limited to the study of the biomarkers induced by occupational or environmental exposure to pesticide mixtures which include the IP. − …”

Section: Discussionmentioning

confidence: 98%

“…43 By contrast, the MNi test in Tradescantia pollen tetrads showed negative results. 44 The literature about IP genotoxicity in human cells is limited to the study of the biomarkers induced by occupational or environmental exposure to pesticide mixtures which include the IP. 45−48 Among the study models conducted to know about the different toxic effects of xenobiotics, the guidelines of the Organization for Economic Coordination and Development, OECD 473, 49 recommend the human cell lines for the chromosome aberrations' analysis as a resource that allows one to study such responses at a cellular level and identify mechanistic aspects related to the molecular interaction.…”

Section: ■ Discussionmentioning

confidence: 99%

Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione

Chaufan

Galvano

Nieves

et al. 2019

Chem. Res. Toxicol.

View full text Add to dashboard Cite

The fungicide agents are a key component in the fruits and vegetables production. The Iprodione residues are one of the pesticide more frequently found in food products. The available data about the cytotoxicity of iprodione and its metabolites are scarce and do not allow characterization of its genotoxic potential and define the risk assessment.The human larynx epidermoid carcinoma cell line (HEp-2) has been shown to be sensitive to the toxic effects of xenobiotics of different origin and have been often used in citotoxicity and genotoxicity studies. The purpose of this paper is to evaluate the induction of genotoxicity and the role of oxidative stress in HEp-2cell line by exposure to the IP. The MTT test for viability resulted in CL50 85.86 (77.05–95.68) μg/mL of Iprodione. On the basis of this result, we proceeded to expose the cells to the sublethal concentrations (below the CL50) during 24 h to analyze the mitotic index and nuclear division index in order to determine the subcytotoxic concentrations of IP which the genotoxicity was evaluated. The subcytotoxic concentrations of 7, 17, and 25 μg/mL IP induced aneugenic effects as micronuclei centromere positive whereas 17 μg/mL was a threshold for centromere negative micronuclei induction in HEp-2 cells. The abnormal mitosis was induced for exposition of Hep-2 cells to the three concentrations. According to the result obtained, citotoxicity and genotoxicity oxidative stress studies were performed in 1.5, 7.0, and 25 μg/mL of IP. The results showed that the GSH intracellular content, the SOD activity and the levels of oxidative damage of the proteins were affected lead to redox imbalance. The decreased in the SOD activity and protein oxidation were in according to the result obtained to genotoxicity, suggesting that different biological targets could be affected.

show abstract

“…1D). Some studies with Tradescantia have shown that the MN test is significantly more sensitive than PC (Kong, Ma, 1999;Fadic et al, 2017).…”

Section: Genotoxicity Effects Of Water/dmso Soil Extracts In Tradescamentioning

confidence: 99%

Soil Genotoxicity Biomonitoring in Recultivated Factory Area Using the Cytogenetic and Molecular Assays in Two Plant Test-Systems

Stapulionytė

Bondzinskaitė

Stravinskaitė

et al. 2018

Proccedings of International Scientific Conference "RURAL DEVELOPMENT 2017"

View full text Add to dashboard Cite

Soil pollution with industrial leftovers is of real danger to living organisms since harmful effects can arise after exposure to the contaminants in the soil. In our study, we applied a plant bioassay battery to monitor soil genotoxicity after short-term exposure to the soil. The soil was collected in 3 rounds: at the central part of the brownfield before (S-I) and after (S-III) topsoil removal, and at the brownfield periphery (S-II). The permissible value of the total contamination index is &amp;lt;16 and the corresponding values were 780 in S-I, 69 in S-II and 133 in S-III soil showing that whole brownfield territory is extremely polluted with heavy metals. Cytogenetic markers were recorded in Allium and Tradescantia test-systems and two types of molecular markers, RAPD and ISSR, were analysed in Allium. Our results revealed that the most polluted soil sample has induced an alarming increase of apoptotic cells in onion roots. Chromosome aberration and micronuclei frequency in Allium decreased inconsistently along with the pollution reduction in the soil. Increased frequencies of all cytogenetic markers were revealed in Tradescantia cuttings after exposure to the S-I soil extracts. Cluster analysis of Allium RAPD and ISSR markers showed that the most polluted soil samples induced genetic changes in onions different from those induced by the least polluted soil. Both plant test-systems in this study confirm that soil from the brownfield is harmful to plants and is potentially hazardous to humans.

show abstract

Tradescantia as a biomonitor for pesticide genotoxicity evaluation of iprodione, carbaryl, dimethoate and 4,4′-DDE

Cited by 20 publications

References 43 publications

A Simple Counting-Based Measurement for Paper Analytical Devices and Their Application

A Simple Counting-Based Measurement for Paper Analytical Devices and Their Application

Oxidative Response and Micronucleus Centromere Assay in HEp-2 Cells Exposed to Fungicide Iprodione

Soil Genotoxicity Biomonitoring in Recultivated Factory Area Using the Cytogenetic and Molecular Assays in Two Plant Test-Systems

Contact Info

Product

Resources

About