The
fungicide agents are a key component in the fruits and vegetables
production. The Iprodione residues are one of the pesticide more frequently
found in food products. The available data about the cytotoxicity
of iprodione and its metabolites are scarce and do not allow characterization
of its genotoxic potential and define the risk assessment.The human
larynx epidermoid carcinoma cell line (HEp-2) has been shown to be
sensitive to the toxic effects of xenobiotics of different origin
and have been often used in citotoxicity and genotoxicity studies.
The purpose of this paper is to evaluate the induction of genotoxicity
and the role of oxidative stress in HEp-2cell line by exposure to
the IP. The MTT test for viability resulted in CL50 85.86
(77.05–95.68) μg/mL of Iprodione. On the basis of this
result, we proceeded to expose the cells to the sublethal concentrations
(below the CL50) during 24 h to analyze the mitotic index
and nuclear division index in order to determine the subcytotoxic
concentrations of IP which the genotoxicity was evaluated. The subcytotoxic
concentrations of 7, 17, and 25 μg/mL IP induced aneugenic effects
as micronuclei centromere positive whereas 17 μg/mL was a threshold
for centromere negative micronuclei induction in HEp-2 cells. The
abnormal mitosis was induced for exposition of Hep-2 cells to the
three concentrations. According to the result obtained, citotoxicity
and genotoxicity oxidative stress studies were performed in 1.5, 7.0,
and 25 μg/mL of IP. The results showed that the GSH intracellular
content, the SOD activity and the levels of oxidative damage of the
proteins were affected lead to redox imbalance. The decreased in the
SOD activity and protein oxidation were in according to the result
obtained to genotoxicity, suggesting that different biological targets
could be affected.
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