Tract-tracing in the nervous system of vertebrates using horseradish peroxidase and its conjugates: tracers, chromogens and stabilization for light and electron microscopy

Want, J.J.L. van der; Klooster, Jan; Cardozo, Bob Nunes; Weerd, Henk de; Liem, Rsb

doi:10.1016/s1385-299x(96)00042-6

Cited by 43 publications

(31 citation statements)

References 35 publications

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Section: A Fibers Were Associated With the Mean Size Of Neurons Relatmentioning

confidence: 99%

“…HRP can be taken up by axons via endocytosis, which can be enhanced by cholera toxin B subunit (CB) and wheat germ agglutinin (WGA) (Kuan et al 2007;Van der Want et al 1997). CB-HRP can be axonally transported both in anterograde and in retrograde directions (Van der Want et al 1997). Low doses of CB-HRP can reveal a CNS neuron's soma, dendrites and axons clearly, and the imaging can be maintained long time (Kuan et al 2007;Ranson et al 1998).…”

Section: A Fibers Were Associated With the Mean Size Of Neurons Relatmentioning

confidence: 99%

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A afferent fibers are involved in the pathology of central changes in the spinal dorsal horn associated with myofascial trigger spots in rats

Meng

Wang

et al. 2015

Exp Brain Res

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A afferent fibers have been reported to participate in the development of the central sensitization induced by inflammation and injuries. Current evidence suggests that myofascial trigger points (MTrPs) induce central sensitization in the related spinal dorsal horn, and clinical studies indicate that A fibers are associated with pain behavior. Because most of these clinical studies applied behavioral indexes, objective evidence is needed. Additionally, MTrP-related neurons in dorsal root ganglia and the spinal ventral horn have been reported to be smaller than normal, and these neurons were considered to be related to A fibers. To confirm the role of A fibers in MTrP-related central changes in the spinal dorsal horn, we studied central sensitization as well as the size of neurons associated with myofascial trigger spots (MTrSs, equivalent to MTrPs in humans) in the biceps femoris muscle of rats and provided some objective morphological evidence. Cholera toxin B subunit-conjugated horseradish peroxidase was applied to label the MTrS-related neurons, and tetrodotoxin was used to block A fibers specifically. The results showed that in the spinal dorsal horn associated with MTrS, the expression of glutamate receptor (mGluR1α/mGluR5/NMDAR1) increased, while the mean size of MTrS-related neurons was smaller than normal. After blocking A fibers, these changes reversed to some extent. Therefore, we concluded that A fibers participated in the development and maintenance of the central sensitization induced by MTrPs and were related to the mean size of neurons associated with MTrPs in the spinal dorsal horn.

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Section: A Fibers Were Associated With the Mean Size Of Neurons Relatmentioning

confidence: 99%

Section: A Fibers Were Associated With the Mean Size Of Neurons Relatmentioning

confidence: 99%

A afferent fibers are involved in the pathology of central changes in the spinal dorsal horn associated with myofascial trigger spots in rats

Meng

Wang

et al. 2015

Exp Brain Res

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“…Both conjugated and unconjugated HRP can be reacted by incubating tissue sections in a chromogen and a substrate such as H 2 O 2 . Various chromogens are available to visualize HRP (see Carson and Mesulam, 1982;Van der Want et al, 1997), and different chromogens can be combined for multi-labeling experiments. For an extensive review of HRP as a neural tracer, refer to Mesulam (1982).…”

Section: Histochemical Detection Of Neural Tracersmentioning

confidence: 99%

Correlative microscopy: A powerful tool for exploring neurological cells and tissues

Czymmek

2011

Micron

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“…All POD molecules can diffuse randomly throughout the cell wall (Carpin et al, 1999). HRP has been widely used as a tracer for neural pathways because it can be absorbed by nerve cell of animal (van der Want et al, 1997;Waar et al, 1981).…”

Section: Figmentioning

confidence: 99%

Modulation of endogenous peroxidase by exogenous peroxidase in chinese red radish seedling

Wang

et al. 2011

Hortic. Environ. Biotechnol.

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To investigate the peroxidase function and its signal pathway, activity and isozyme pattern of peroxidase and transcription level of the peroxidase gene rsprx1 and rsprx2 in Chinese red radish seedling (Raphanus sativus L.) were investigated after administration of the exogenous peroxidase (extract of radish). The activity and isozyme pattern of peroxidases were increased, among cotyledon, hypocotyl and root, the most significant increase of peroxidases activity was observed in hypocotyls, the activity of phenylalanine ammonialyase was also increased, but the pelargonidin content was decreased. Although the exogenous application of horseradish peroxidase and the extract of radish could both affect the activity of endogenous enzyme and metabolites of Chinese red radish seedlings, the latter was more effective than the former. Nifedipine, as L-calcium channel blocker, affected the activity of peroxidase and pelargonidin content in Chinese red radish seedling. Exogenously added nifedipine with the extract caused reversal of the effects. It is concluded that calcium was involved in the signal pathway of the extract of radish in Chinese red radish seedling.Additional key words: horseradish peroxidase, nifedipine, pelargonidin, Raphanus sativus L.

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Tract-tracing in the nervous system of vertebrates using horseradish peroxidase and its conjugates: tracers, chromogens and stabilization for light and electron microscopy

Cited by 43 publications

References 35 publications

A afferent fibers are involved in the pathology of central changes in the spinal dorsal horn associated with myofascial trigger spots in rats

A afferent fibers are involved in the pathology of central changes in the spinal dorsal horn associated with myofascial trigger spots in rats

Correlative microscopy: A powerful tool for exploring neurological cells and tissues

Modulation of endogenous peroxidase by exogenous peroxidase in chinese red radish seedling

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