Correlative microscopy: A powerful tool for exploring neurological cells and tissues

Czymmek, Kirk J.

doi:10.1016/j.micron.2011.07.001

Cited by 28 publications

(9 citation statements)

References 187 publications

(301 reference statements)

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“…Multiple, complementary imaging modalities are often used to obtain a more complete picture of the subject or sample under study. An example in bioimaging is the correlative recording of structural and functional image data, using electron and fluorescence microscopy, respectively [298] , [299] , [300] . But it does not stop there.…”

Section: Discussionmentioning

confidence: 99%

A bird’s-eye view of deep learning in bioimage analysis

Meijering

2020

Computational and Structural Biotechnology Journal

112

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Deep learning of artificial neural networks has become the de facto standard approach to solving data analysis problems in virtually all fields of science and engineering. Also in biology and medicine, deep learning technologies are fundamentally transforming how we acquire, process, analyze, and interpret data, with potentially far-reaching consequences for healthcare. In this mini-review, we take a bird’s-eye view at the past, present, and future developments of deep learning, starting from science at large, to biomedical imaging, and bioimage analysis in particular.

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Section: Discussionmentioning

confidence: 99%

A bird’s-eye view of deep learning in bioimage analysis

Meijering

2020

Computational and Structural Biotechnology Journal

112

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“…Given that fluorescence allows the imaging of specific moieties with extremely high SNRs, as well as of live cells, combining these techniques with EM to obtain ultrastructural context is appealing. Several groups have reported advances recently, primarily involving the combination of fluorescence microscopy with TEM (at either room or cryogenic temperatures) or scanning electron microscopy, and these have been reviewed elsewhere [66][67][68][69][70][71] . Combining fluorescence microscopy with use of the FIB-SEM allows the user to investigate larger volumes; however, there are several technical challenges related to sample preparation and post-processing of images.…”

Section: Correlative Imaging With the Fib-semmentioning

confidence: 99%

Focused ion beams in biology

2015

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A quiet revolution is under way in technologies used for nanoscale cellular imaging. Focused ion beams, previously restricted to the materials sciences and semiconductor fields, are rapidly becoming powerful tools for ultrastructural imaging of biological samples. Cell and tissue architecture, as preserved in plastic-embedded resin or in plunge-frozen form, can be investigated in three dimensions by scanning electron microscopy imaging of freshly created surfaces that result from the progressive removal of material using a focused ion beam. The focused ion beam can also be used as a sculpting tool to create specific specimen shapes such as lamellae or needles that can be analyzed further by transmission electron microscopy or by methods that probe chemical composition. Here we provide an in-depth primer to the application of focused ion beams in biology, including a guide to the practical aspects of using the technology, as well as selected examples of its contribution to the generation of new insights into subcellular architecture and mechanisms underlying host-pathogen interactions.

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“…However, combining fluorescence and electron microscopy remains the most common form of correlative microscopy. Different aspects of correlative fluorescence and electron microscopy have been discussed in a number of excellent reviews and articles; the reader is referred to these for a broader discussion of correlative microscopy (e.g., [5,10,24,40,42,58,67,68,70,83,84,92,109]). Herein, we will focus on correlative fluorescence and electron microscopy using the bifunctional probe, FluoroNanogold (FNG).…”

Section: Introductionmentioning

confidence: 99%

FluoroNanogold: an important probe for correlative microscopy

et al. 2015

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Correlative microscopy is a powerful imaging approach that refers to observing the same exact structures within a specimen by two or more imaging modalities. In biological samples, this typically means examining the same sub-cellular feature with different imaging methods. Correlative microscopy is not restricted to the domains of fluorescence microscopy and electron microscopy; however, currently, most correlative microscopy studies combine these two methods, and in this review, we will focus on the use of fluorescence and electron microscopy. Successful correlative fluorescence and electron microscopy requires probes, or reporter systems, from which useful information can be obtained with each of the imaging modalities employed. The bi-functional immunolabeling reagent, FluoroNanogold, is one such probe that provides robust signals in both fluorescence and electron microscopy. It consists of a gold cluster compound that is visualized by electron microscopy and a covalently attached fluorophore that is visualized by fluorescence microscopy. FluoroNanogold has been an extremely useful labeling reagent in correlative microscopy studies. In this report, we present an overview of research using this unique probe.

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Correlative microscopy: A powerful tool for exploring neurological cells and tissues

Cited by 28 publications

References 187 publications

A bird’s-eye view of deep learning in bioimage analysis

A bird’s-eye view of deep learning in bioimage analysis

Focused ion beams in biology

FluoroNanogold: an important probe for correlative microscopy

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