Focused ion beams in biology

Narayan, Kedar; Subramaniam, Sriram

doi:10.1038/nmeth.3623

Cited by 200 publications

(158 citation statements)

References 108 publications

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“…213 SEM provides lateral resolution on the order of 1.0 nm and can also provide elemental information useful in labeling experiments. Because transmission is not required as with TEM, SEM can image tissues of millimeter-scale thickness.…”

Section: Probing Cellular Interactions Of Nanoparticlesmentioning

confidence: 99%

Cellular uptake of nanoparticles: journey inside the cell

et al. 2017

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Nanoscale materials are increasingly found in consumer goods, electronics, and pharmaceuticals. While these particles interact with the body in myriad ways, their beneficial and/or deleterious effects ultimately arise from interactions at the cellular and subcellular level. Nanoparticles (NPs) can modulate cell fate, induce or prevent mutations, initiate cell-cell communication, and modulate cell structure in a manner dictated largely by phenomena at the nano-bio interface. Recent advances in chemical synthesis have yielded new nanoscale materials with precisely defined biochemical features, and emerging analytical techniques have shed light on nuanced and context-dependent nano-bio interactions within cells. In this review, we provide an objective and comprehensive account of our current understanding of the cellular uptake of NPs and the underlying parameters controlling the nano-cellular interactions, along with the available analytical techniques to follow and track these processes.

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Section: Probing Cellular Interactions Of Nanoparticlesmentioning

confidence: 99%

Cellular uptake of nanoparticles: journey inside the cell

et al. 2017

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“…FIB milling has become a highly established technique to prepare cross-sectional slice lamellas for high-resolution transmission electron microscopy (TEM) [6,7]. The popular realization of FIB tools are arranged in a dualbeam configuration, i.e.…”

Section: Focused Ion Beamsmentioning

confidence: 99%

“…Apart from making cross-sections, mechanical manipulatorequipped FIB systems are used in preparing TEM lamellas [6]. However, even recently, the slow rate of FIB slicing and alteration of samples are listed as future development needs in bio-applications of FIB [7].…”

Section: Introductionmentioning

confidence: 99%

Tipping solutions: emerging 3D nano-fabrication/ -imaging technologies

et al. 2017

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The evolution of optical microscopy from an imaging technique into a tool for materials modification and fabrication is now being repeated with other characterization techniques, including scanning electron microscopy (SEM), focused ion beam (FIB) milling/imaging, and atomic force microscopy (AFM). Fabrication and in situ imaging of materials undergoing a three-dimensional (3D) nano-structuring within a 1−100 nm resolution window is required for future manufacturing of devices. This level of precision is critically in enabling the cross-over between different device platforms (e.g. from electronics to micro-/ nano-fluidics and/or photonics) within future devices that will be interfacing with biological and molecular systems in a 3D fashion. Prospective trends in electron, ion, and nano-tip based fabrication techniques are presented.

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“…Thus, while serial sectioning involves careful non-linear section-to-section registration [35], blockface techniques deliver aligned high-resolution image stacks through large volumes. Indeed, SBEM and FIB-SEM have both already been used to image volumes of brain and other tissues [38][39][40][41].…”

Section: Electron Microscopy Of Volumesmentioning

confidence: 99%