Tracking the Impact of Excisional Cervical Treatment on the Cervix using Biospectroscopy

Abstract

Local excisional treatment for cervical intra-epithelial neoplasia (CIN) is linked to significant adverse sequelae including preterm birth, with cone depth and radicality of treatment correlating to the frequency and severity of adverse events. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy can detect underlying cervical disease more accurately than conventional cytology. The chemical profile of cells pre- and post-treatment may differ as a result of altered biochemical processes due to excision, or treatment of the disease. Since pre-treatment cervical length varies amongst women, the percentage of cervix excised may correlate more accurately to risk than absolute dimensions. We show that treatment for CIN significantly alters the biochemistry of the cervix, compared with women who have not had treatment; this is due to the removal of cervical tissue rather than the removal of the disease. However, the spectra do not seem to correlate to the cone depth or proportion of cervical length excised. Future research should aim to explore the impact of treatment in a larger cohort.

Clinical Observation of rhIL-2 Combined with Zhenqi Fuzheng and BaofuKang Suppository in the Treatment of Cervical Intraepithelial Neoplasia II with HPV Infection

“…Even when low-grade cervical intraepithelial neoplasia (CIN1) develops, the majority of these lesions regress spontaneously back to normal, particularly in young women [4]. While they have cervical CIN, in terms of CINII, CINIII, most of them will be treated with cervical conization and physical lesion, such as cold knife conization (CKC), large loop excision of the transformation zone (LLETZ), hysteroscopic conization of cervix and laser conization and so on [5]. Moreover, postoperative complications, such as wound bleeding, infection, cervical incompetence and cervical adhesions, will affect future fertility, and the cost is high.…”

An imaging dataset of cervical cells using scanning near-field optical microscopy coupled to an infrared free electron laser

“…A summary of both classification systems is presented in Supplementary Tables 1 and 2 for squamous and glandular lesions, respectively. An overview of how cervical cytology and histology are determined is provided, as are images for cervical cytology and histology for normal, low-grade dyskaryosis, high-grade dyskaryosis and squamous and glandular cervical cancers 18,19,20,21,22 .…”

“…Images for cervical cytology and histology of normal, squamous and glandular lesions are provided 18,19,20,21,22 .…”

Terminology for cone dimensions after local conservative treatment for cervical intraepithelial neoplasia and early invasive cervical cancer: 2022 consensus recommendations from ESGO, EFC, IFCPC, and ESP

“…The volumetric fluid displacement technique is likely to represent the most accurate measurement (the gold standard), whereby the difference in fluid level before and after the immersion of a cone is recorded (Archimedes' principle; appendix p 6). 15,17,[36][37][38][39][40][41][42] When this technique is not feasible, researchers have used various formulas to calculate the volume of a cone from its dimensions. 16,32,[43][44][45][46][47][48][49][50][51][52][53][54] As the shape of cones can vary, researchers have used different cone shapes to determine volume, such as a cone, a semi-ellipsoid, an ellipsoid, a parallelepiped, and a truncated cone (frustum; appendix pp 2-3, 7-8).…”