An imaging dataset of cervical cells using scanning near-field optical microscopy coupled to an infrared free electron laser

Halliwell, Diane E.; Morais, Camilo L. M.; Lima, Kássio M. G.; Trevisan, Júlio; Siggel-King, M. R. F.; Craig, Timothy; Ingham, James; Martin, David S.; Heys, Kelly; Kyrgiou, Maria; Mitra, Anita; Paraskevaidis, Evangelos; Theophilou, Georgios; Martin‐Hirsch, Pierre L.; Cricenti, A.; Luce, M.; Weightman, P.; Martin, Francis

doi:10.1038/sdata.2017.84

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…ATR-FtIR and Raman spectroscopy were used to categorise disease and identify cancer or intra-epithelial neoplasia in a number of excised tissues, such as prostate [97-101], gastrointestinal tract [102][103][104][105][106], brain [95, 107,108], breast [109][110][111][112][113][114][115][116], lung [117,118] and skin [119][120][121]. Gynaecological applications include studies of cervical cytology and histopathology [122][123][124][125][126][127], ovarian cancer [128,129] and vulvar disease [130].…”

Section: Biospectroscopy For Endometrial Tissue Interrogationmentioning

confidence: 99%

New Insights into Endometrial Cancer 2022

2023

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Commons Attribution (CC BY) license, which allows users to download, copy and build upon published articles, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. Preface to "New Insights into Endometrial Cancer 2022"Endometrial cancer is a hormone-dependent cancer with an increasing incidence that is estimated to continue to grow over the next several years. It is typically treated with surgery and/or chemo-/radiation therapy. The clinical benefit of hormone therapies for advanced and recurrent endometrial cancers underlines the need to examine their characteristics (particularly, steroid hormone receptor expression and functions) to assess how they can be used better. Furthermore, a critical phase that can drive clinicians' therapeutic choices is histopathological and molecular classification. Thus, a current challenge is to integrate IHC markers with molecular tests to identify prognostic groups. Moreover, the observations of the immunosuppressive nature of the endometrial cancer environment are leading to studies that assess therapies with the aim to boost the immune response, which might represent significant potential in the treatment of the disease. For this reason, current and ongoing studies are trying to improve clinical responses through immunotherapy strategies, either alone or combined with classic treatments.

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Section: Biospectroscopy For Endometrial Tissue Interrogationmentioning

confidence: 99%

New Insights into Endometrial Cancer 2022

2023

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“…ATR-FtIR and Raman spectroscopy were used to categorise disease and identify cancer or intra-epithelial neoplasia in a number of excised tissues, such as prostate [97][98][99][100][101], gastrointestinal tract [102][103][104][105][106], brain [95,107,108], breast [109][110][111][112][113][114][115][116], lung [117,118] and skin [119][120][121]. Gynaecological applications include studies of cervical cytology and histopathology [122][123][124][125][126][127], ovarian cancer [128,129] and vulvar disease [130].…”

Section: Biospectroscopy For Endometrial Tissue Interrogationmentioning

confidence: 99%

Vibrational Biospectroscopy: An Alternative Approach to Endometrial Cancer Diagnosis and Screening

Schiemer

Furniss

Phang

et al. 2022

IJMS

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Endometrial cancer (EC) is the sixth most common cancer and the fourth leading cause of death among women worldwide. Early detection and treatment are associated with a favourable prognosis and reduction in mortality. Unlike other common cancers, however, screening strategies lack the required sensitivity, specificity and accuracy to be successfully implemented in clinical practice and current diagnostic approaches are invasive, costly and time consuming. Such limitations highlight the unmet need to develop diagnostic and screening alternatives for EC, which should be accurate, rapid, minimally invasive and cost-effective. Vibrational spectroscopic techniques, Mid-Infrared Absorption Spectroscopy and Raman, exploit the atomic vibrational absorption induced by interaction of light and a biological sample, to generate a unique spectral response: a “biochemical fingerprint”. These are non-destructive techniques and, combined with multivariate statistical analysis, have been shown over the last decade to provide discrimination between cancerous and healthy samples, demonstrating a promising role in both cancer screening and diagnosis. The aim of this review is to collate available evidence, in order to provide insight into the present status of the application of vibrational biospectroscopy in endometrial cancer diagnosis and screening, and to assess future prospects.

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“…Presently, IR-FELs are open at many synchrotron-radiation facilities all over the world since the optical emission was first observed at a laboratory level more than 50 years ago [ 31 ]. Various original experimental studies using IR-FELs are reported as follows: ablation of biological tissues [ 32 , 33 , 34 ], alteration of biological functions [ 35 , 36 ], degradation of protein–metal complexes [ 37 , 38 ], optical and spectroscopic imaging [ 39 , 40 , 41 ], structural dynamics of biomolecules [ 42 , 43 ], chemical reactions in the gas phase [ 44 , 45 , 46 ], decomposition of biopolymers [ 47 , 48 , 49 ], nonlinear-optical physics [ 50 ], and observation of terahertz radiation [ 51 ].…”

Section: Introductionmentioning

confidence: 99%

Disassembly of Amyloid Fibril with Infrared Free Electron Laser

Kawasaki

Tsukiyama

Nguyen

2023

IJMS

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Amyloid fibril causes serious amyloidosis such as neurodegenerative diseases. The structure is composed of rigid β-sheet stacking conformation which makes it hard to disassemble the fibril state without denaturants. Infrared free electron laser (IR-FEL) is an intense picosecond pulsed laser that is oscillated through a linear accelerator, and the oscillation wavelengths are tunable from 3 μm to 100 μm. Many biological and organic compounds can be structurally altered by the mode-selective vibrational excitations due to the wavelength variability and the high-power oscillation energy (10–50 mJ/cm2). We have found that several different kinds of amyloid fibrils in amino acid sequences were commonly disassembled by the irradiation tuned to amide I (6.1–6.2 μm) where the abundance of β-sheet decreased while that of α-helix increased by the vibrational excitation of amide bonds. In this review, we would like to introduce the IR-FEL oscillation system briefly and describe combination studies of experiments and molecular dynamics simulations on disassembling amyloid fibrils of a short peptide (GNNQQNY) from yeast prion and 11-residue peptide (NFLNCYVSGFH) from β2-microglobulin as representative models. Finally, possible applications of IR-FEL for amyloid research can be proposed as a future outlook.

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An imaging dataset of cervical cells using scanning near-field optical microscopy coupled to an infrared free electron laser

Cited by 4 publications

References 26 publications

New Insights into Endometrial Cancer 2022

New Insights into Endometrial Cancer 2022

Vibrational Biospectroscopy: An Alternative Approach to Endometrial Cancer Diagnosis and Screening

Disassembly of Amyloid Fibril with Infrared Free Electron Laser

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