Tracking of Cellular Uptake of Hydrophilic CdSe/ZnS Quantum Dots/Hydroxyapatite Composites Nanoparticles in MC3T3-E1 Osteoblast Cells

Hsieh, Ming‐Fa; Li, Jimmy Kuan-Jung; Lin, Cheng-An J.; Huang, Shin-Huei; Sperling, Ralph A.; Parak, Wolfgang J.; Chang, Walter

doi:10.1166/jnn.2009.463

Cited by 23 publications

(13 citation statements)

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“…cell dead or decreased cellular activity) effect (Huerta-García et al 2015). A variety of NPs have thus been demonstrated to be internalized by osteoblasts, such as calcium phosphate nano shells (Schmidt et al 2008), implants´wear debris (Lohmann et al 2000), quantum dot/hydroxyapatite composites (Hsieh et al 2009), polymeric particles (Tautzenberger et al 2011). Gold NPs have been shown as an effective material with no osteogenesis potential, nor apoptosis to osteoblasts (the MG63 cell line) (Tsai et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

et al. 2017

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Covalent conjugation of (bis)phosphonate group-containing molecules, sodium Alendronate (Aln) and 3-AminoropylPhosphoric Acid (ApA), to Cellulose nanocrystals (CNCs) was performed via oxidation/Shiff-base reaction. Further fluorescent labelling with Rhodamine B Iso ThioCyanate (RBITC) was performed to follow CNCs interaction and potential internalization with/in human osteoblasts by confocal microscopy. Complementary analyses were applied to identify the conjugation (Atenuated Total Reflectance-Fourier Transform Infrared and UV-VIS spectroscopies), physico-chemical (Dynamic Light Scattering and Nanoparticle Tracking Analysis) and morphological (Transmission Electron Microscopy) features of native and ApA/Aln-modified CNCs in physiologically relevant environments (Phosphate Buffer Saline, Advanced Dulbecco's Modified Eagle Medium). While conjugation did not affect the CNCs' size, the RBITC-labelling promotes their aggregation. Faster (1 h vs. 2 h) uptake by osteoblasts of RBITCCNCoxAln, compared to RBITC-CNCoxApA, and no-internalization (in 24 h) of native RBITTC-CNC, indicate a higher affinity of Aln-modified CNCs to the cells, while all CNCs (in 0.25-0.06 wt%) promote the cell growth. Aln/Apa-modified CNCs shows high potential in drug-delivery for bone therapies, and theranostics.

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Section: Introductionmentioning

confidence: 99%

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

et al. 2017

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“…Interestingly, the fluorescence was only displayed in the cytoplasm of MC3T3-E1 osteoblast cells. 129 Gold NPs have recently been investigated as contrast agents for micro-CT applications 130,131 and advanced X-ray imaging technologies. 132 Since Au has a higher absorption coefficient than Iodine and experiences less interference with bones and tissues, Au NPs improved contrast at lower doses while prolonging the imaging time.…”

Section: Diagnosismentioning

confidence: 99%

Nanomedicine applications in orthopedic medicine: state of the art

Mazaheri¹,

Eslahi²,

Ordikhani³

et al. 2015

IJN

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The technological and clinical need for orthopedic replacement materials has led to significant advances in the field of nanomedicine, which embraces the breadth of nanotechnology from pharmacological agents and surface modification through to regulation and toxicology. A variety of nanostructures with unique chemical, physical, and biological properties have been engineered to improve the functionality and reliability of implantable medical devices. However, mimicking living bone tissue is still a challenge. The scope of this review is to highlight the most recent accomplishments and trends in designing nanomaterials and their applications in orthopedics with an outline on future directions and challenges.

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“…Similar to MSCs, osteoblasts were demonstrated to internalize a variety of nanoparticles, such as wear debris, 109 quantum dot/hydroxyapatite composites, 110 calcium phosphate nanoshells, 111 and polymeric particles. 112 Again, various factors influenced nanoparticle uptake and potential cellular effects.…”

Section: Osteoblastsmentioning

confidence: 99%

Nanoparticles and their potential for application in bone

Tautzenberger¹,

Kovtun²,

Ignatius³

2012

IJN

157

137

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Abstract:Biomaterials are commonly applied in regenerative therapy and tissue engineering in bone, and have been substantially refined in recent years. Thereby, research approaches focus more and more on nanoparticles, which have great potential for a variety of applications. Generally, nanoparticles interact distinctively with bone cells and tissue, depending on their composition, size, and shape. Therefore, detailed analyses of nanoparticle effects on cellular functions have been performed to select the most suitable candidates for supporting bone regeneration. This review will highlight potential nanoparticle applications in bone, focusing on cell labeling as well as drug and gene delivery. Labeling, eg, of mesenchymal stem cells, which display exceptional regenerative potential, makes monitoring and evaluation of cell therapy approaches possible. By including bioactive molecules in nanoparticles, locally and temporally controlled support of tissue regeneration is feasible, eg, to directly influence osteoblast differentiation or excessive osteoclast behavior. In addition, the delivery of genetic material with nanoparticulate carriers offers the possibility of overcoming certain disadvantages of standard protein delivery approaches, such as aggregation in the bloodstream during systemic therapy. Moreover, nanoparticles are already clinically applied in cancer treatment. Thus, corresponding efforts could lead to new therapeutic strategies to improve bone regeneration or to treat bone disorders.

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Tracking of Cellular Uptake of Hydrophilic CdSe/ZnS Quantum Dots/Hydroxyapatite Composites Nanoparticles in MC3T3-E1 Osteoblast Cells

Cited by 23 publications

References 0 publications

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

Nanomedicine applications in orthopedic medicine: state of the art

Nanoparticles and their potential for application in bone

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