Tracking adoptive natural killer cells via ultrasound imaging assisted with nanobubbles

Jiang, Yizhou; Hou, Xuandi; Zhao, Xin; Jing, Jianing; Sun, Lei

doi:10.1016/j.actbio.2023.07.058

Cited by 5 publications

(2 citation statements)

References 50 publications

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“…A scheme like ours may be broadly applied to identify cells that show increased mechanosensitivity than surrounding tissue, such as in aging brains or the progression of cancers 55 , 56 . The ease of the GVs’ surface modification could also enable cellular targeting through attached ligands, or other targeting molecules 57 , 58 . Thus, the ability of PGVs-mediated ultrasound stimulation to act upon mechanosensitive ion channels could be applied in fields beyond neurostimulation.…”

Section: Discussionmentioning

confidence: 99%

Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice

Hou,

Jing,

Jiang

et al. 2024

Nat Commun

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Ultrasound is an acoustic wave which can noninvasively penetrate the skull to deep brain regions, enabling neuromodulation. However, conventional ultrasound’s spatial resolution is diffraction-limited and low-precision. Here, we report acoustic nanobubble-mediated ultrasound stimulation capable of localizing ultrasound’s effects to only the desired brain region in male mice. By varying the delivery site of nanobubbles, ultrasound could activate specific regions of the mouse motor cortex, evoking EMG signaling and limb movement, and could also, separately, activate one of two nearby deep brain regions to elicit distinct behaviors (freezing or rotation). Sonicated neurons displayed reversible, low-latency calcium responses and increased c-Fos expression in the sub-millimeter-scale region with nanobubbles present. Ultrasound stimulation of the relevant region also modified depression-like behavior in a mouse model. We also provide evidence of a role for mechanosensitive ion channels. Altogether, our treatment scheme allows spatially-targetable, repeatable and temporally-precise activation of deep brain circuits for neuromodulation without needing genetic modification.

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Section: Discussionmentioning

confidence: 99%

Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice

Hou,

Jing,

Jiang

et al. 2024

Nat Commun

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“…By activating NKCs to change their morphology, they can be more effectively utilized for immune responses against cancer cells [ 5 , 6 ]. Therefore, quantitative analysis of NKCs’ immunological data to understand their group dynamics is necessary, yet this research is challenging, and related work remains limited [ 7 , 8 , 9 , 10 ]. Accurate detection, classification, and stable tracking of NKCs’ coordinates are imperative for such analysis.…”

Section: Introductionmentioning

confidence: 99%

An Improved Two-Shot Tracking Algorithm for Dynamics Analysis of Natural Killer Cells in Tumor Contexts

Zhou,

Tang,

2024

Bioengineering

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Natural killer cells (NKCs) are non-specific immune lymphocytes with diverse morphologies. Their broad killing effect on cancer cells has led to increased attention towards activating NKCs for anticancer immunotherapy. Consequently, understanding the motion characteristics of NKCs under different morphologies and modeling their collective dynamics under cancer cells has become crucial. However, tracking small NKCs in complex backgrounds poses significant challenges, and conventional industrial tracking algorithms often perform poorly on NKC tracking datasets. There remains a scarcity of research on NKC dynamics. In this paper, we utilize deep learning techniques to analyze the morphology of NKCs and their key points. After analyzing the shortcomings of common industrial multi-object tracking algorithms like DeepSORT in tracking natural killer cells, we propose Distance Cascade Matching and the Re-Search method to improve upon existing algorithms, yielding promising results. Through processing and tracking over 5000 frames of images, encompassing approximately 300,000 cells, we preliminarily explore the impact of NKCs’ cell morphology, temperature, and cancer cell environment on NKCs’ motion, along with conducting basic modeling. The main conclusions of this study are as follows: polarized cells are more likely to move along their polarization direction and exhibit stronger activity, and the maintenance of polarization makes them more likely to approach cancer cells; under equilibrium, NK cells display a Boltzmann distribution on the cancer cell surface.

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Precise modulation of cell activity using sono-responsive nano-transducers

Hou,

Liu,

Sun

2024

Biomaterials

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Tracking adoptive natural killer cells via ultrasound imaging assisted with nanobubbles

Cited by 5 publications

References 50 publications

Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice

Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice

An Improved Two-Shot Tracking Algorithm for Dynamics Analysis of Natural Killer Cells in Tumor Contexts

Precise modulation of cell activity using sono-responsive nano-transducers

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