Tracheal responsiveness to both isoprenaline and beta<sub>2</sub>‐adrenoreceptor blockade by propranolol in cigarette smoke exposed and sensitized guinea pigs

Boskabady, Mohammad Hossein; Kiani, Sahar; Aslani, Mohammad Reza

doi:10.1111/j.1440-1843.2005.00893.x

Cited by 26 publications

(29 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tracheal chains of Dunkin-Hartley guinea pigs were prepared as previously described [23] and suspended in a 10 ml organ bath (organ bath 61300, Bio Science Palmer-Washington, Sheerness, Kent, UK) containing Krebs-Henseleit solution with known composition [23] which was maintained at 37° and gassed with 95% O 2 and 5% CO 2 .…”

Section: Animals and Tissue Preparationsmentioning

confidence: 99%

Portulaca olerace Affects Muscarinic Receptors of Guinea Pig Tracheal Smooth Muscle

Hashemzehi¹,

Khazdair²

2016

pharmaceutical-sciences

View full text Add to dashboard Cite

Hashemzehi, et al.: Effect of Portulaca oleracea on Musacrinic ReceptorsThe relaxant effect of Portulaca oleracea L. on tracheal smooth muscles has been shown. The effect of the plant on muscarinic receptors of tracheal smooth muscles as one possible mechanism responsible for its relaxant effect was examined. Concentration response curves to methacholine were performed in the presence of three concentrations of aqueous-ethanol extract from aerial part of the plant (0.25, 0.50 and 1.00 mg/ml), 10 nM atropine, and saline. The values of EC 50 (effective concentration causing 50% of maximum response) and CR-1 ((EC 50 of test solutions/EC 50 of saline)-1) were measured. The study was done using three designs as non-incubated (group 1), incubated tissues with propranolol and chlorpheniramine (group 2) and incubated tissues with propranolol (group 3). Concentration-response curves to methacholine were shifted to the right and EC50 methacholine were significantly higher in the presence of atropine, medium and highest extract concentrations in all groups and its low concentration in group 3 compared to saline. In addition, EC 50 methacholine in the presence of high extract concentration (27.00±2.12) in group 2 was greater than its low (10.33±1.54) and medium (16.83±2.52) concentrations (p<0.05). The values of the CR-1 obtained in the presence of all extract concentrations in all groups were lower than that of atropine (P<0.05 to P<0.001). An inhibitory effect for the extract of P. oleracea on muscarinic receptors of tracheal smooth muscles was shown and a histamine (H 1 ) receptor blockade was also suggested.Key words: Portulaca oleracea, muscarinic receptor, tracheal smooth muscle, inhibitory effect, aqueousethanol extract Portulaca oleracea L. is an annual succulent which may reach 15.75 inches in height with alternate leaves clustered at stem joints; the yellow flower plant grows in different areas of the world including north and northwest of Iran [1] . Main active constituents of P. oleracea are omega-3 fatty acid, α-linolenic acid and eicosa-pentaenoic acid. This plant also contains vitamins (mainly vitamins A, C and some vitamin B) [2,3] . The plant also contains organic acids such as citric, maleic, cyanuric, caffeic acid as well as flavonoids, coumarins, and glycosides [4] .P. oleracea has several therapeutic effects including diuretic, antiascorbic, antipyretic, antiinflammatory [5][6][7] . The plant is also used for treatment of type-2 diabetes mellitus patients [8] . Different pharmacologic effects of this plant are antiinflammatory, analgesic [6] , antioxidant [9][10][11] , potassium channel opener and blood pressure modifier [12] , as well as neuropharmacological [13,14] , wound healing [15] , antibacterial [16] and antipyretic effects [17] . The relaxant effect of this plant was studied on skeletal muscles [18] and smooth muscle of small intestine [19] . Our previous studies also showed relaxant effect of the plant on tracheal smooth muscle [20] and the possible mechanism of this effect for this plant [21]...

show abstract

Section: Animals and Tissue Preparationsmentioning

confidence: 99%

Portulaca olerace Affects Muscarinic Receptors of Guinea Pig Tracheal Smooth Muscle

Hashemzehi¹,

Khazdair²

2016

pharmaceutical-sciences

View full text Add to dashboard Cite

show abstract

“…It gets accumulated in embryonic organs such as kidney (2). Cigarette smoke increased upper airway tracts sensitivity to isoprenaline (3). Furthermore, nicotine exposure in the embryonic period resulted in decreased fibronectin expression in pulmonary parenchyma (4).…”

Section: Introductionmentioning

confidence: 99%

Fibronectin Regulation by Vitamin C Treatment in Kidneys of Nicotinic Mice Offspring

Pahang¹,

Nikravesh²,

Jalali³

et al. 2014

Iran Red Crescent Med J

View full text Add to dashboard Cite

Background:Maternal cigarette smoking causes health risks and developmental defects in the offspring. So far, many studies have been conducted to suppress the effects of nicotine. However, the effects of coadministration of vitamin C and nicotine on extracellular matrix have not gained enough attention.Objectives:This study decided to investigate the effects of vitamin C on fibronectin expression in kidneys of mice offspring, treated with nicotine.Materials and Methods:Eighteen female pregnant BALB/c mice were selected; six mice in the experimental group 1 (exp 1) received nicotine (3 mg/kg/day), six mice in the experimental group 2 (exp 2) received 3 mg/kg/day nicotine and 9 mg/kg/day vitamin C simultaneously, and six were used as the control group and received 3 mL/kg/day normal saline via intraperitoneal (IP) injection parallel to other groups, since the 6th day of gestation to the end of prenatal period. In the first days of delivery, fibronectin content of neonatal kidneys was studied by immunohistochemistry (IHC) assay and gene expression was studied by the real-time PCR.Results:IHC results showed that fibronectin reaction significantly increased in proximal convoluted tubules of exp 1 compared with the control offspring; on the other hand, fibronectin reaction decreased in the mice offspring of exp 2. Gene expression results showed that fibronectin expression in the exp 1 offspring significantly increased compared with the control ones and fibronectin expression decreased in the mice offspring of exp 2.Conclusions:This study revealed that vitamin C could reduce the fibronectin accumulation effects of nicotine on kidney.

show abstract

“…[12] This hypothesis was then tested with another β-agonist (salbutamol) in asthmatic patients and also in smokers. [13,14] This phenomenon was also demonstrated in tracheal chains of sensitized and COPD animals [30,31] as well as epithelial denuded trachea. [32] In most of the above-mentioned studies a close correlation was found between airway responsiveness to methacholine and β-agonist.…”

Section: Discussionmentioning

confidence: 94%

Pharmacologic bronchodilation response to salbutamol in copd patients

Boskabady

Mansouri

et al. 2010

Indian J Med Sci

View full text Add to dashboard Cite

show abstract

Tracheal responsiveness to both isoprenaline and beta₂‐adrenoreceptor blockade by propranolol in cigarette smoke exposed and sensitized guinea pigs

Cited by 26 publications

References 42 publications

Portulaca olerace Affects Muscarinic Receptors of Guinea Pig Tracheal Smooth Muscle

Portulaca olerace Affects Muscarinic Receptors of Guinea Pig Tracheal Smooth Muscle

Fibronectin Regulation by Vitamin C Treatment in Kidneys of Nicotinic Mice Offspring

Pharmacologic bronchodilation response to salbutamol in copd patients

Contact Info

Product

Resources

About

Tracheal responsiveness to both isoprenaline and beta2‐adrenoreceptor blockade by propranolol in cigarette smoke exposed and sensitized guinea pigs

Cited by 26 publications

References 42 publications

Portulaca olerace Affects Muscarinic Receptors of Guinea Pig Tracheal Smooth Muscle

Portulaca olerace Affects Muscarinic Receptors of Guinea Pig Tracheal Smooth Muscle

Fibronectin Regulation by Vitamin C Treatment in Kidneys of Nicotinic Mice Offspring

Pharmacologic bronchodilation response to salbutamol in copd patients

Contact Info

Product

Resources

About

Tracheal responsiveness to both isoprenaline and beta₂‐adrenoreceptor blockade by propranolol in cigarette smoke exposed and sensitized guinea pigs