Fibronectin Regulation by Vitamin C Treatment in Kidneys of Nicotinic Mice Offspring

Abstract

Background:Maternal cigarette smoking causes health risks and developmental defects in the offspring. So far, many studies have been conducted to suppress the effects of nicotine. However, the effects of coadministration of vitamin C and nicotine on extracellular matrix have not gained enough attention.Objectives:This study decided to investigate the effects of vitamin C on fibronectin expression in kidneys of mice offspring, treated with nicotine.Materials and Methods:Eighteen female pregnant BALB/c mice were selected; six mice in the experimental group 1 (exp 1) received nicotine (3 mg/kg/day), six mice in the experimental group 2 (exp 2) received 3 mg/kg/day nicotine and 9 mg/kg/day vitamin C simultaneously, and six were used as the control group and received 3 mL/kg/day normal saline via intraperitoneal (IP) injection parallel to other groups, since the 6th day of gestation to the end of prenatal period. In the first days of delivery, fibronectin content of neonatal kidneys was studied by immunohistochemistry (IHC) assay and gene expression was studied by the real-time PCR.Results:IHC results showed that fibronectin reaction significantly increased in proximal convoluted tubules of exp 1 compared with the control offspring; on the other hand, fibronectin reaction decreased in the mice offspring of exp 2. Gene expression results showed that fibronectin expression in the exp 1 offspring significantly increased compared with the control ones and fibronectin expression decreased in the mice offspring of exp 2.Conclusions:This study revealed that vitamin C could reduce the fibronectin accumulation effects of nicotine on kidney.

Protective Effects of Vitamin C against Nicotine-Induced Oxidative Damage of Rat Liver and Kidney

“…Previous researchers found that abnormal nuclear shape is associated with premature and normal aging, as well as cancer [41] [42] [43]. The changes observed in the kidney are greatly improved by the use of vitamin C. Consistent with our results; one study revealed that vitamin C antagonized nicotine-induced kidney damage through reduction of fibronectin accumulation in proximal tubules of the kidney [44].…”

Effect of chronic sodium nitrite administration on the expression of fibronectin in interstitial tissue of mice testis: An immunohistochemical study

“…Total RNA Extraction: The testicular tissue that had been stored in RNA-later solution at -70°C, was thawed at room temp and underwent the following steps according to the RNA extraction kit protocol [12].…”

“…cDNA Synthesis: The cDNA was synthesized as per the instructions and protocol provided by the cDNA Synthesis Easy Kit manufacturer [12]. In the first stage, 5 µL of total RNA, 2 µL of Oligo-dt and 1 µL of Random hexamer were mixed in a microtube.…”

“…The whole RT-PCR procedure was performed according to the protocol advised by the manufacturer. According to the protocol, a suitable amount of DW was added to each tube containing the forward and reverse primers to obtain a solution with a concentration of 100 from each to prepare a primer of 10 Pmol dilution [12].…”

“…High concentrations of nitrite and nitrate affect the fertility of men by changing the parameters of sperm. Half of the infertility problems are related to men [12]. To our knowledge, no study has been conducted on the possible effects of water contaminants on the expression of fibronectin in testicular interstitial tissue.…”