Tracheal expression of Osiris gene family in Drosophila

Scholl, Aaron; Yang, Yuyang; McBride, Patrick E.; Irwin, Kelly E.; Jiang, Lan

doi:10.1016/j.gep.2018.03.001

Cited by 11 publications

(18 citation statements)

References 34 publications

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“…To obtain information about the function of Osiris genes, we investigated the mRNA expression of 24 Osiris genes in Drosophila embryos by in situ hybridization. We found that all of the Osi genes were expressed at variable levels and patterns in late embryonic stages when the cuticle is secreted, in various tissues, including the epidermis, mouth parts, foregut, hindgut, trachea, and spiracles (Figures 4A and S4; Table S1; see also [26,27] for a subset of Osi gene expression in trachea). We classified the Osiris expression patterns into 8 types, which collectively covered essentially all of the cuticle-producing organs of first-instar larvae (Figure S4).…”

Section: Resultsmentioning

confidence: 97%

Nanopore Formation in the Cuticle of an Insect Olfactory Sensillum

et al. 2019

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Section: Resultsmentioning

confidence: 97%

Nanopore Formation in the Cuticle of an Insect Olfactory Sensillum

et al. 2019

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“…We also detected GFP within the lumen near the base of the sensillum shaft (Figure 3I), which may correspond to the occasional protrusions of the tormogen cell and/or extracellular vacuoles that likely derive from this cell type (Nava Gonzales et al, 2021). Confirmation of this localization pattern will require development of specific Osi8 antibodies to detect endogenous protein, but the distribution of GFP:Osi8 in various membranous organelle-like structures is similar to that of other Osi proteins (Ando et al, 2019; Lee et al, 2013; Scholl et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Comparative transcriptomics of the Drosophila olfactory subsystems identifies a support cell-expressed Osiris protein required for pheromone sensing

Scalzotto

Cruchet

et al. 2022

Preprint

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The nose of most animals comprises multiple sensory subsystems, which are defined by the expression of different olfactory receptor families. Drosophila melanogaster antennae comprise two morphologically and functionally distinct subsystems that express Odorant receptors (Ors) or Ionotropic receptors (Irs). Although these receptors have been thoroughly characterized in this species, the subsystem-specific expression and roles of other genes are much less well-understood. Here we generate subsystem-specific transcriptomic datasets to identify hundreds of genes, encoding diverse protein classes, that are selectively enriched in either Or or Ir subsystems. Using single-cell antennal transcriptomic data and RNA in situ hybridization, we find most neuronal genes - other than sensory receptor genes - are broadly expressed within the subsystems. By contrast, we identify many non-neuronal genes that exhibit highly selective cell-type expression, revealing substantial molecular heterogeneity in the non-neuronal cellular components of these olfactory subsystems. We characterize one Or subsystem-specific non-neuronal molecule, Osiris 8 (Osi8), a conserved member of a large family of insect transmembrane proteins. Osi8 is expressed in tormogen support cells that are associated with pheromone sensing neurons. Loss of Osi8 abolishes high sensitivity neuronal responses to pheromone ligands. Together this work identifies a new protein required for insect pheromone detection, emphasizes the importance of support cells in sensory responses, and provides a resource for future characterization of other olfactory subsystem-specific genes.

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“…4A, Table S1, Fig. S4, supplementary text, see also [25,26] for a subset of Osi gene expression in trachea). We classified the Osiris expression patterns into 8 types, which collectively covered essentially all of the cuticle-producing organs of first-instar larvae ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Nanopore formation in the cuticle of an insect olfactory sensillum

Ando

Sekine

Inagaki

et al. 2018

Preprint

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Nanometer-level patterned surface structures form the basis of biological functions including superhydrophobicity, structural coloration, and light absorption [1][2][3]. In insects, the cuticle overlying the olfactory sensilla has multiple small (50-200-nm diameter) pores [4][5][6][7][8], which are 25 supposed to function as a filter that admits odorant molecules, while preventing the entry of larger airborne particles and limiting water loss. However, the cellular processes underlying the patterning of extracellular matrices into functional nano-structures remain unknown. Here we show that cuticular nanopores in Drosophila olfactory sensilla originate from a curved ultrathin film that is formed in the outermost envelope layer of the cuticle, and secreted from specialized 30 protrusions in the plasma membrane of the hair forming (trichogen) cell. The envelope curvature coincides with plasma membrane undulations associated with endocytic structures. The goretex/Osiris23 gene encodes an endosomal protein that is essential for envelope curvature, nanopore formation, and odor receptivity, and is expressed specifically in developing olfactory trichogen cells. The 24-member Osiris gene family is expressed in cuticle-secreting cells, and is 35 found only in insect genomes. These results reveal an essential requirement for nanopores for Ando et al., February 12, 2019 2 odor reception and identify Osiris genes as a platform for investigating the evolution of surface nano-fabrication in insects. Results and DiscussionThe great success of insects in spreading throughout nearly the entire terrestrial ecosystem has 5been largely due to their exoskeleton, which enabled both their adaptation to harsh environments and protection from predators. The adaptation of olfaction to airborne odorants was also key in helping insects search for food, mates, and other environmental cues, and to establish social communication [9]. Insect sensilla consist of one to several neurons and usually three supporting or auxiliary cells. The neuron(s) innervate a specialized cuticular apparatus, which often has the 10 shape of a peg or hair and is secreted by the auxiliary cells. Later in development, these cells take over other functions, e.g. secretion of the sensillum lymph that surrounds the dendrites of the neurons. In olfactory sensilla the wall of the cuticular hair shows numerous pores which are absent from sensilla serving other modalities. For volatile odorants to reach the dendrites, numerous pores ranging in diameter from 50 to 200 nm are formed in regular arrays on the 15 cuticles of the olfactory sensory organs [4][5][6][7][8]. Beneath the pores, filaments called pore tubules extend to the interior lymph and dendritic nerve endings [7,8]. These nano-scale structures are thought to allow the diffusion of small odorant molecules (0.5 to 5 nm in diameter) to reach the olfactory neurons, while preventing the entry of larger (100 to 1000 nm) airborne particles and minimizing the loss of inner lymph liquid. This selective filter system pro...

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Tracheal expression of Osiris gene family in Drosophila

Cited by 11 publications

References 34 publications

Nanopore Formation in the Cuticle of an Insect Olfactory Sensillum

Nanopore Formation in the Cuticle of an Insect Olfactory Sensillum

Comparative transcriptomics of the Drosophila olfactory subsystems identifies a support cell-expressed Osiris protein required for pheromone sensing

Nanopore formation in the cuticle of an insect olfactory sensillum

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