Nanopore Formation in the Cuticle of an Insect Olfactory Sensillum

Ando, Tsuneya; Sekine, Sayaka; Inagaki, Sachi; Misaki, Kazuyo; Badel, Laurent; Moriya, Hajime; Sami, Mustafa M.; Itakura, Yuki; Chihara, Takahiro; Kazama, Hokto; Yonemura, Shigenobu; Hayashi, Shigeo

doi:10.1016/j.cub.2019.03.043

Cited by 33 publications

(51 citation statements)

References 47 publications

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“…Different sizes and spatial patterns of olfactory pores in a given morphological type of olfactory sensilla have not been reported in other insects. In fruit flies, the formation of olfactory pores is controlled by a specific gene for cuticle secretion in single-walled olfactory sensilla 39 . Thus, olfactory pores of the cockroach might also be formed by specific gene control.…”

Section: Discussionmentioning

confidence: 99%

Sensory neurons that respond to sex and aggregation pheromones in the nymphal cockroach

Tateishi

Nishimura

Sakuma

et al. 2020

Sci Rep

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in the common pest cockroach, Periplaneta americana, behavioural responses to the sex and aggregation pheromones change in an age-dependent manner. nymphs are attracted by the aggregation pheromone periplanolide-e (pLD-e) but not by the sex pheromone periplanone-B (pB) in faeces. Adults display prominent behaviours to PB but not to PLD-E. Despite the significant behavioural differences depending on postembryonic developmental stages, peripheral codings of the sex and aggregation pheromones have not been studied in the nymph of any insects as far as we know. in this study, we morphologically and electrophysiologically identified antennal sensilla that respond to PB and pLD-e in nymphal cockroaches. Although nymphs lacked the sex pheromone-responsive singlewalled B (sw-B) sensilla identified in adult males, we found PB-responsive sensory neurons (PB-SNs) within newly identified sw-A2 sensilla, which exhibit different shapes but have the same olfactory pores as sw-B sensilla. Interestingly, PLD-E-responsive sensory neurons (PLD-E-SNs) were also identified in the same sensillar type, but PB and PLD-E were independently detected by different SNs. Both PB-SNs and pLD-e-Sns showed high sensitivity to their respective pheromones. the hemimetabolous insect nymph has an ability to detect these pheromones, suggesting that behaviours elicited by pheromones might be established in brain centres depending on postembryonic development.

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Section: Discussionmentioning

confidence: 99%

Sensory neurons that respond to sex and aggregation pheromones in the nymphal cockroach

Tateishi

Nishimura

Sakuma

et al. 2020

Sci Rep

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“…Here, we show that the Orco is specifically enriched in the part of the branched OS that is housed inside the porous cuticle shaft. Nano-pores of the cuticle shaft, which houses the ciliary OS containing the branched extensions [ 20 , 25 ], is essential for odour sensing [ 41 ]. The branching also increases the surface area of the cilia and accommodates a larger number of the receptors.…”

Section: Discussionmentioning

confidence: 99%

Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages

et al. 2021

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The cilium, the sensing centre for the cell, displays an extensive repertoire of receptors for various cell signalling processes. The dynamic nature of ciliary signalling indicates that the ciliary entry of receptors and associated proteins must be regulated and conditional. To understand this process, we studied the ciliary localisation of the odour-receptor coreceptor (Orco), a seven-pass transmembrane protein essential for insect olfaction. Little is known about when and how Orco gets into the cilia. Here, using Drosophila melanogaster, we show that the bulk of Orco selectively enters the cilia on adult olfactory sensory neurons in two discrete, one-hour intervals after eclosion. A conditional loss of heterotrimeric kinesin-2 during this period reduces the electrophysiological response to odours and affects olfactory behaviour. We further show that Orco binds to the C-terminal tail fragments of the heterotrimeric kinesin-2 motor, which is required to transfer Orco from the ciliary base to the outer segment and maintain within an approximately four-micron stretch at the distal portion of the ciliary outer-segment. The Orco transport was not affected by the loss of critical intraflagellar transport components, IFT172/Oseg2 and IFT88/NompB, respectively, during the adult stage. These results highlight a novel developmental regulation of seven-pass transmembrane receptor transport into the cilia and indicate that ciliary signalling is both developmentally and temporally regulated.

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“…Interestingly, other members of the Osi family are expressed in cuticle-secreting cells elsewhere in the fly (e.g. those lining the tracheae), hinting at a common role for this insect-specific protein family in shaping cuticular structures [28].…”

Section: The Morphology and Cell Biology Of Olfactory Sensillamentioning

confidence: 99%

Molecular mechanisms of olfactory detection in insects: beyond receptors

Schmidt

Benton

2020

Open Biol.

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Insects thrive in diverse ecological niches in large part because of their highly sophisticated olfactory systems. Over the last two decades, a major focus in the study of insect olfaction has been on the role of olfactory receptors in mediating neuronal responses to environmental chemicals. In vivo , these receptors operate in specialized structures, called sensilla, which comprise neurons and non-neuronal support cells, extracellular lymph fluid and a precisely shaped cuticle. While sensilla are inherent to odour sensing in insects, we are only just beginning to understand their construction and function. Here, we review recent work that illuminates how odour-evoked neuronal activity is impacted by sensillar morphology, lymph fluid biochemistry, accessory signalling molecules in neurons and the physiological crosstalk between sensillar cells. These advances reveal multi-layered molecular and cellular mechanisms that determine the selectivity, sensitivity and dynamic modulation of odour-evoked responses in insects.

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Nanopore Formation in the Cuticle of an Insect Olfactory Sensillum

Cited by 33 publications

References 47 publications

Sensory neurons that respond to sex and aggregation pheromones in the nymphal cockroach

Sensory neurons that respond to sex and aggregation pheromones in the nymphal cockroach

Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages

Molecular mechanisms of olfactory detection in insects: beyond receptors

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