Tracer diffusion in crowded solutions of sticky polymers

Macromolecular cryoprotectants for the preservation of mammalian cell culture: lessons from crowding, overview and perspectives

“…156 Moreover, techniques like FCS and FRAP spectroscopy will allow researchers to study macromolecular diffusion in crowded freezing media. 157 Interestingly, spatiotemporal analysis of intracellular macromolecular crowding is possible due to techniques like fluorescence anisotropy imaging microscopy (FAIM) and fluorescence lifetime imaging microscopy (FLIM) as well as by a method involving the use of genetically encoded protein sensor to quantify crowding by fluorescence resonance energy transfer (FRET). 158,159 These methods are anticipated to assess the extent of intracellular crowding by osmotic dehydration due to macromolecular CPAs in cells.…”

In Silico Studies of Active Probe Dynamics in Crowded Media

“…In cellular processes, the combined efforts of multiple factors maintain the smooth functioning of the cell and its biological functions. Thus, in real-life situations, there exist many factors that significantly affect the dynamics or the transportation of probe particles, such as activity, size, and shape of the probe particles, and its interactions with the local environment. ,, Among these processes, the macromolecular transport through the cellular channels in biological systems is of interest to researchers, where, along with crowding, confinement plays a crucial role. Cellular channels, like the nuclear pore complex (NPC), allow a bidirectional transport of ions and proteins across the nuclear envelope by passive diffusion through the channels.…”

“…The dynamics of active Brownian particles become the central focus of the physical and biophysical research communities. In the last few decades, several studies are reported, which try to reveal the physics behind the novel features exhibited by the active Brownian particles in complex media. ,− However, the real-life situations are quite different, and hence there is a growing interest in understanding the dynamics of these active Brownian particles in complex environments such as porous soils, sediments, and living tissues. There are multiple factors like crowding and local heterogeneity that either enhance or limit the diffusion of active agents moving through these complex media for performing their assigned tasks.…”

Hard core-soft shell particles near repulsive interfaces: interplay between adsorption, aggregation and diffusion

“…When confined or near surfaces, this anomalous behavior is affected by the surface. [66][67][68][69] To see how the colloids diffuses at the interface or in the bulk we have evaluated the lateral mean square displacement (LMSD) for distinct layer. With this, we obtain the lateral diffusion constant D l for each layer, with l ranging from 1, the contact layer, to 15, the layer exactly at the simulation box center where the density was fixed in ρ 0 .…”