Traceability of mussel (Mytilus chilensis) in southern Chile using microsatellite molecular markers and assignment algorithms. Exploratory survey

Larraín, María Angélica; Díaz, Nelson F.; Lamas, Carmen; Uribe, Carla; Araneda, Cristián

doi:10.1016/j.foodres.2014.02.016

Cited by 37 publications

(46 citation statements)

References 76 publications

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“…A number of methods and analytical procedures have been applied to individual tracing and identification of populations . Likelihood and exclusion approaches are commonly used to track individuals .…”

Section: Introductionmentioning

confidence: 99%

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Tracing individuals and populations of the tambaqui, Colossoma macropomum (Cuvier, 1818), from Brazilian hatcheries using microsatellite markers

et al. 2019

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BACKGROUND In recent years, tracing of alimentary produce of animal origin has become increasingly important, for economic, food safety and ecological reasons. The tambaqui, Colossoma macropomum, is the native fish most farmed in Brazil. The reliable identification of the origin of tambaquis (wild or farmed) offered for sale to the general public has become necessary to satisfy regulatory norms and uphold consumer confidence. Molecular methods based on the analysis of DNA sequences have often been used to evaluate the potential for tracing farmed fish, given their reliability and precision. RESULTS Full likelihood and Bayesian approaches proved to be the most efficient for the identification, respectively, of individuals and populations for most of the fish sampled from seven hatcheries and one wild stock. The exclusion method and genetic distances were the least effective approaches for the identification of individuals and populations. The Bayesian method identified correctly more than 99% of the fry from most stocks, except those of the Santarém hatchery and River Amazon wild stock, which presented the best results for individual identification. CONCLUSIONS The identification of populations was effective for most hatcheries, although the identification of individuals from most stocks was hampered by the reduced genetic variability. © 2018 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

“…For likelihood analyses, the Cervus v3.0 and Colony v2.0.6.4 programs are typically used, while exclusion analyses are run in Vitassign v8.5 . The identification of populations is based on genetic distances, allelic frequencies and Bayesian analyses, run in programs such as Geneclass v2.0 …”

Section: Introductionmentioning

confidence: 99%

Tracing individuals and populations of the tambaqui, Colossoma macropomum (Cuvier, 1818), from Brazilian hatcheries using microsatellite markers

et al. 2019

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“…Most studies have relied on molecular techniques, including PCR1112, FINS1314 and DNA barcoding1516, for species identification. Molecular tools, particularly microsatellites1718, as well as biochemical methods, such as fatty acids192021 and stable isotopes212223, have also been used to assess geographical origin of bivalves. Trace element fingerprinting (TEF) of bivalve mineral structures may also be useful to distinguish populations or stocks242526.…”

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confidence: 99%

Trace element fingerprinting of cockle (Cerastoderma edule) shells can reveal harvesting location in adjacent areas

Ricardo

Génio

Leal

et al. 2015

Sci Rep

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Determining seafood geographic origin is critical for controlling its quality and safeguarding the interest of consumers. Here, we use trace element fingerprinting (TEF) of bivalve shells to discriminate the geographic origin of specimens. Barium (Ba), manganese (Mn), magnesium (Mg), strontium (Sr) and lead (Pb) were quantified in cockle shells (Cerastoderma edule) captured with two fishing methods (by hand and by hand-raking) and from five adjacent fishing locations within an estuarine system (Ria de Aveiro, Portugal). Results suggest no differences in TEF of cockle shells captured by hand or by hand-raking, thus confirming that metal rakes do not act as a potential source of metal contamination that could somehow bias TEF results. In contrast, significant differences were recorded among locations for all trace elements analysed. A Canonical Analysis of Principal Coordinates (CAP) revealed that 92% of the samples could be successfully classified according to their fishing location using TEF. We show that TEF can be an accurate, fast and reliable method to determine the geographic origin of bivalves, even among locations separated less than 1 km apart within the same estuarine system. Nonetheless, follow up studies are needed to determine if TEF can reliably discriminate between bivalves originating from different ecosystems.

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“…The high levels of polymorphism observed at many STR markers (microsatellites, or SSRs), alongside their establishment in human DNA forensics, has led to their use in determining geographic origin in a range of wildlife species including African ivory [26,27], bobcats in the USA [28], Sardinian mouflon [29], tortoise [30] and bears [31], as well as fish [32,33], molluscs [34] and plants [35]. In well differentiated populations (F ST > 0.1), a relatively low number of microsatellite markers (n = 10) would likely provide sufficient assignment power for identification of population origin, however in more weakly structured populations, the number of markers required to assign unknown samples with confidence rises rapidly.…”

Section: Microsatellitesmentioning

confidence: 99%

Wildlife forensic science: A review of genetic geographic origin assignment

Ogden

Linacre

2015

Forensic Science International: Genetics

122

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Traceability of mussel (Mytilus chilensis) in southern Chile using microsatellite molecular markers and assignment algorithms. Exploratory survey

Cited by 37 publications

References 76 publications

Tracing individuals and populations of the tambaqui, Colossoma macropomum (Cuvier, 1818), from Brazilian hatcheries using microsatellite markers

Tracing individuals and populations of the tambaqui, Colossoma macropomum (Cuvier, 1818), from Brazilian hatcheries using microsatellite markers

Trace element fingerprinting of cockle (Cerastoderma edule) shells can reveal harvesting location in adjacent areas

Wildlife forensic science: A review of genetic geographic origin assignment

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