Trace the profile and function of circular RNAs in Sertoli cell only syndrome

Zhu, Fang; Luo, Yang; Bo, Hao; Gong, Guanghui; Tang, Ruiling; Fan, Jingyu; Zhang, Huan; Liu, Gang; Zhu, Wenbing; Tan, Yue‐Qiu; He, Zuping; Fan, Liqing

doi:10.1016/j.ygeno.2021.04.022

Cited by 12 publications

(8 citation statements)

References 44 publications

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“… 35 In a recent study, Zhu et al . 36 explored the circRNA expression pattern in the testicular tissues of patients with SCOS and found that host genes of the differentially expressed circRNAs (DEcircRNAs) were enriched in biological processes related to the cell cycle and intercellular communication. In the present study, we found a unique differential circRNA expression profile in the testes of patients with NOA compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of circular RNAs in the testicular tissue of patients with non-obstructive azoospermia

Liang¹,

Jiang²,

Zhang³

et al. 2022

Asian Journal of Andrology

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Circular RNAs (circRNAs) are highly conserved and ubiquitously expressed noncoding RNAs that participate in multiple reproduction-related diseases. However, the expression pattern and potential functions of circRNAs in the testes of patients with non-obstructive azoospermia (NOA) remain elusive. In this study, according to a circRNA array, a total of 37 881 circRNAs were identified that were differentially expressed in the testes of NOA patients compared with normal controls, including 19 874 upregulated circRNAs and 18 007 downregulated circRNAs. Using quantitative real-time polymerase chain reaction (qRT-PCR) analysis, we confirmed that the change tendency of some specific circRNAs, including hsa_circ_0137890, hsa_circ_0136298, and hsa_circ_0007273, was consistent with the microarray data in another larger sample. The structures and characteristics of these circRNAs were confirmed by Sanger sequencing, and fluorescence in situ hybridization revealed that these circRNAs were primarily expressed in the cytoplasm. Bioinformatics analysis was used to construct the competing endogenous RNA (ceRNA) network, and numerous miRNAs that could be paired with circRNAs validated in this study were reported to be vital for spermatogenesis regulation. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses indicated that genes involved in axoneme assembly, microtubule-based processes, and cell proliferation were significantly enriched. Our data suggest that there are aberrantly expressed circRNA profiles in patients with NOA and that these circRNAs may help identify key diagnostic and therapeutic molecular biomarkers for NOA patients.

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Section: Discussionmentioning

confidence: 99%

Identification and characterization of circular RNAs in the testicular tissue of patients with non-obstructive azoospermia

Liang¹,

Jiang²,

Zhang³

et al. 2022

Asian Journal of Andrology

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“…More and more studies have shown that circRNA is related to NOA, mainly through competitive endogenous RNA network (ceRNA) to participate in the occurrence of NOA. One study identified 399 circRNA up-regulated and 1195 down-regulated by High throughput circRNA microarray analysis, constructed an up-regulated ceRNA of hsa-circRNA-101373 and identified multiple miRNA targets, which are involved in the processes of apoptosis, cell cycle arrest and spermatogenesis ( 81 ). Similarly, a study identified 19874 up-regulated circRNA and 18007 down-regulated circRNAs in the testis of patients with NOA by circRNA array, constructed a ceRNA network, and found that miRNAs paired with circRNAs is essential for the regulation of spermatogenesis ( 82 ).…”

Section: Circrnamentioning

confidence: 99%

The gene regulatory role of non-coding RNAs in non-obstructive azoospermia

Zhou

Zhang

et al. 2022

Front. Endocrinol.

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Non-coding RNAs are classified as small non-coding RNAs, long non-coding RNAs and circular RNAs, which are involved in a variety of biological processes, including cell differentiation, proliferation, apoptosis and pathological conditions of various diseases. Many studies have shown that non-coding RNAs are related to spermatogenesis, maturation, apoptosis, function, etc. In addition, the expression of non-coding RNAs in testicular tissue and semen of patients with non-obstructive azoospermia was different. However, the role of non-coding RNAs in the pathogenesis of non-obstructive azoospermia has not been fully elucidated, and the role of non-coding RNAs in non-obstructive azoospermia is rarely reviewed. Here we summarize the research progress of non-coding RNAs in the pathogenesis of non-obstructive azoospermia.

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“…10,11 For example, circRNAs involved in the development of many diseases, such as embryonic heart, 12 neurological disorders 13,14 and male infertility. 15,16 However, the roles of circRNAs in cleft palates by TCDD induce were still unknown. Therefore, identification of circRNAs can help us to explore the molecular mechanisms of cleft palate by TCDD induced.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of differentially expressed circRNA in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cleft palate

et al. 2023

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Various circular RNAs (circRNAs) are novel class of non-coding RNAs, which are pervasively transcribed in the genome. CircRNAs play important roles in human, animals and plants. Up to now, there was no report regarding circRNAs of cleft palate by 2,3,7,8-tetrachlorodibenzo-pdioxin (TCDD) induce. The present study screened identification and characterization of differential expressed-circRNAs in TCDD-induced cleft palate. 6903 circRNAs candidates came from cleft palates. Among them, 3525 circRNAs are up-regulation, and 3378 circRNAs are down-regulation by TCDD induce. The cluster and GO analysis found that circRNAs involved in biological process, cellular component, and molecular function. Through the analysis of KEGG Pathway, circRNAs made functions via classical signaling pathway in cleft palate, such as TGF-beta signaling pathway, BMP signal pathway, MAPK signaling pathway. In addition, we found down-regulated circRNA224, circRNA3302 and up-regulated circRNA5021 targeted tgfbr3, but up-regulated circRNA4451 targeted tgfbr2. circRNA4451 may make functions through TGF-beta signaling pathway. These results suggested that many different circRNAs may make important role in TCDD-induced cleft palate, which provided a theoretical basis for further research.

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Trace the profile and function of circular RNAs in Sertoli cell only syndrome

Cited by 12 publications

References 44 publications

Identification and characterization of circular RNAs in the testicular tissue of patients with non-obstructive azoospermia

Identification and characterization of circular RNAs in the testicular tissue of patients with non-obstructive azoospermia

The gene regulatory role of non-coding RNAs in non-obstructive azoospermia

Identification and characterization of differentially expressed circRNA in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cleft palate

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