The gene regulatory role of non-coding RNAs in non-obstructive azoospermia

Zhou, Guanqing; Zhang, Mimi; Zhang, Jingzhi; Feng, Yaofeng; Xie, Zhishen; Liu, Siyi; Zhu, Detu; Luo, Yuan

doi:10.3389/fendo.2022.959487

Cited by 5 publications

(3 citation statements)

References 98 publications

(96 reference statements)

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“…The common causes of NOA include congenital chromosomal abnormalities, gene mutations, and varicocele, but the specific pathogenesis of NOA remains unknown ( Peña et al, 2020 ). A previous study found that lncRNA participates in the progression of NOA ( Zhou et al, 2022 ). LINC00467 upregulated the expression levels of genes related to spermatogenesis ( TDRD6 and LRGUK ) to promote the progression of NOA ( Bo et al, 2020 ).…”

Section: Reproductive Function Diseases Potentially Related To Lncrna...mentioning

confidence: 99%

Mechanism of LncRNA Gm2044 in germ cell development

Zhu,

Sun,

et al. 2024

Front. Cell Dev. Biol.

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Germ cell development in mammals is a complex physiological process that involves the proliferation of primordial germ cells, meiosis, and the formation of male and female gametes. Long non-coding RNA (lncRNA) is a type of RNA with more than 200 nucleotides that does not code for proteins. A small number of lncRNAs have been shown to participate in spermatogenesis in the testes and in follicular development in the ovaries, but the role of the vast majority of lncRNAs and their molecular mechanisms still need further study. LncRNA Gm2044 was identified as a differentially expressed lncRNA in mouse spermatogenesis by microarray technology. In mouse testis, lncRNA Gm2044 can act as competing endogenous RNA to regulate SYCP1 expression in GC-2 cells derived from mouse spermatocyte cells, and it can also act as a host gene for miR-202 to regulate RBFOX2 protein expression. In female mouse ovaries, lncRNA Gm2044 regulates 17β-estradiol synthesis through the miRNA-138-5p-Nr5a1 pathway or by interacting with EEF2. In addition, studies suggest that lncRNA Gm2044 is also involved in the progression of reproductive system diseases such as male nonobstructive azoospermia. Here, we summarize the roles and molecular mechanisms of lncRNA Gm2044 in male and female gametogenesis and its potential role in some infertility disorders.

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Section: Reproductive Function Diseases Potentially Related To Lncrna...mentioning

confidence: 99%

Mechanism of LncRNA Gm2044 in germ cell development

Zhu,

Sun,

et al. 2024

Front. Cell Dev. Biol.

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“…The speci c mechanisms by which lncRNAs regulate miRNAs include: acting as potential pri-miRNAs to generate mature miRNAs, indirectly regulating target gene expression [44]; indirectly inhibiting miRNA-mediated negative regulation of target genes by competitively binding to the 3'-UTR of miRNA target mRNAs [45]; and serving as a "miRNA sponge" to inhibit miRNA expression [46]. On one hand, miRNAs can bind to the 3'-UTR region of lncRNAs, thereby reducing the stability and expression of lncRNAs [47]. On the other hand, miRNAs can return to the cell nucleus and regulate the transcription of lncRNAs, enhancing lncRNA expression.…”

Section: Disscussionmentioning

confidence: 99%

LincRNA-MSTRG.673.2 Promotes Chicken Intramuscular Adipocyte Differentiation by Sponging miR- 128-3p

Zhu#,

Zhang,

et al. 2024

Preprint

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Background Intramuscular fat content is positively correlated with meat flavor and juiciness. Increasing the intramuscular fat (IMF) content of chickens while increasing their growth rate has become a hot topic in molecular breeding.The group's previous studies showed that miR-128-3p inhibited chicken intramuscular adipocyte differentiation and lipogenesis. However, the regulatory mechanism of miR-128-3p in intramuscular preadipocytes is currently unknown. In this study, we investigated the mechanism of miR-128-3p regulation of chicken intramuscular adipocyte differentiation and deposition. Methods RNA-seq was performed to screen for long non-coding RNAs (lncRNAs) that bind to miR-128-3p. Dual luciferase reporter system was used to verify the targeting relationship between miR-128-3p and LincRNA-MSTRG.673.2; nucleoplasmic localization analysis and fluorescence in situ hybridization were used to investigate the localization of LincRNA-MSTRG.673.2 in the cells; a series of experiments such as Q-PCR, Oil O Red staining and triglyceride assay were used to explore the effect of interference with LincRNA-MSTRG.673.2 on the differentiation of intramuscular preadipocytes; co-transfection experiments were used to validate the regulatory patterns of miR-128-3p and LincRNA-MSTRG.673.2 in intramuscular adipocytes. Results Transcriptome data analysis of differential LincRNAs indicated that, compared to the NC group, the mimics-treated group had 17 significantly differentially expressed LincRNAs (P < 0.05), including 6 upregulated and 11 downregulated ones; the inhibitor-treated group had 17 differentially expressed LincRNAs (P < 0.05), including 8 upregulated and 9 downregulated ones; and 24 differentially expressed LincRNAs (P < 0.05) were observed when comparing the mimics-treated group to the inhibitor-treated group, with 14 upregulated and 10 downregulated ones. Functional enrichment analysis revealed that DELincRNAs from the overexpression group (M group) and interference group (SI group) were involved in negative regulation of metabolic processes, response to steroid hormones, regulation of actin cytoskeleton. Furthermore, target gene prediction analysis showed that miR-128-3p can target many of the DELincRNAs, such as LincRNA-MSTRG.673.2, LincRNA-MSTRG.39.2, LincRNA-MSTRG.39.3, and LincRNA-MSTRG.14270.2. LincRNA-MSTRG.673.2 was predominantly expressed in cytoplasm of intramuscular adipocytes. Dual luciferase reporter identified the targeting relationship between miR-128-3p and LincRNA-MSTRG.673.2. The results of subsequent functional assays demonstrated that Interfering with MSTRG.673.2 has been shown to inhibit lipid deposition in intramuscular preadipocytes. Transfection experiments have shown that LincR-MSTRG.673.2 can affect the expression of miR-128-3p. Conclusion This study found that LincRNA-MSTRG.673.2 promoted chicken intramuscular adipocytes differentiation by down regulating miR-128-3p. The results are noteworthy for improving chicken meat quality, molecular breeding, and lipid metabolism research.

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“…MiRNAs as a class of small ncRNAs play a crucial role in downregulating gene expression by competitively interacting with the mRNA 3′UTR region and mediating RNA degradation through the RISC complex ( Zhou et al, 2022 ). MiRNAs have two main applications.…”

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confidence: 99%