Trace Element Determination and Cardioprotection of Terminalia pallida Fruit Ethanolic Extract in Isoproterenol Induced Myocardial Infarcted Rats by ICP-MS

Abstract

The trace elements and minerals in Terminalia pallida fruit ethanolic extract (TpFE) were determined by the instrument inductively coupled plasma-mass spectrometry (ICP-MS), and the cardioprotection of TpFE against isoproterenol (ISO)-administered rats was studied. Rats were pretreated with TpFE (100, 300, and 500 mg/kg bw) for 30 days, with concurrent administration of ISO (85 mg/kg bw) for two consecutive days. The levels of trace elements and minerals in TpFE were below the permitted limits of World Health Organization standards. ISO administration significantly increased the heart weight and cardiac marker enzymes in serum, xanthine oxidase, sodium, and calcium in the heart, whereas significantly decreased body weight, reduced glutathione, glutathione-S-transferase, superoxide dismutase, and potassium in the heart. Oral pretreatment of TpFE significantly prevented the ISO-induced alterations. This is the first report that revealed the determination of trace elements and mineral nutrients of TpFE by ICP-MS which plays a principal role in the herbal drug discovery for the treatment of cardiovascular diseases.

Protective effects of syringic acid, resveratrol and their combination against isoprenaline administered cardiotoxicity in wistar rats

“…Pre-treatment with COMB depressed Na + and Ca 2+ ion levels and elevated K + ion amounts in all ISO challenged rats, demonstrating the antioxidant assets of COMB in opposition to oxidation of SH groups on ATPase enzymes and preventive action of COMB against lipid peroxidation, which imply the membrane stabilization character of COMB. Our results are linear with the previous report (Althaf Hussain et al, 2018).…”

Ameliorative Effects of Ponicidin Against the Isoproterenol-induced Acute Myocardial Infarction in Rats

“…54 Here, our outcomes revealed that the MI rats demonstrated the depletion of K + ions and augmented the Na + and Ca +2 ion concentrations in the cardiac tissues. However, the treatment with the ponicidin remarkably decreased these ion contents and improved the K + ions in the cardiac tissues (Figure 6), which is supported by Hussain et al 55…”

“…54 Here, our outcomes revealed that the MI rats demonstrated the depletion of K + ions and augmented the Na + and Ca +2 ion concentrations in the cardiac tissues. However, the treatment with the ponicidin remarkably decreased these ion contents and improved the K + ions in the cardiac tissues (Figure 6), which is supported by Hussain et al 55 In normal cellular physiological conditions, Na + and K + ATPases maintain the Na + and K + ions transport through the membrane, upon its damage may result in ionic dissimilarity. Consequently, the measurement of the activities of membrane-bound enzymes will denote the changes in membrane physiology under pathological circumstances.…”

The Genus Terminalia (Combretaceae): An Ethnopharmacological, Phytochemical and Pharmacological Review

“…The supplementation of TpFE dose-dependently exerts notable protection on myocardium by virtue of its strong antioxidant activity. It could be used as a medicinal food for the treatment of cardiovascular ailments [163].…”