Trace determination of tetracyclines in water samples by capillary zone electrophoresis combining off-line and on-line sample preconcentration

Moreno‐González, David; Lupión-Enríquez, Isabel; Garcı́a-Campaña, Ana M.

doi:10.1002/elps.201500440

Cited by 30 publications

(15 citation statements)

References 37 publications

(36 reference statements)

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“…The BGE composed of 20 mM HAc and 0.05% Tween 20 at pH 3.3 containing 2 mM acridine as a probe for indirect UV detection at 254 nm provided LODs between 3 and 5 g/L. CE-LVSS with polarity switching (to remove the sample matrix) combined with offline SPE was applied by Moreno-González et al [60] to the analysis of tetracycline antibiotics in river, spring, and ground waters. Using 75 mM sodium carbonate and 1 mM EDTA at pH 10 as the BGE and UV detection at 270 nm, sensitivity enhancement factors ranging from 303 to 428 and LODs between 67 and 167 ng/L were obtained.…”

Section: Environmental Analysesmentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

2018

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Electrophoretic sample stacking comprises a group of capillary electrophoretic techniques where trace analytes from the sample are concentrated into a short zone (stack). This paper is a continuation of our previous reviews on the topic and brings a survey of more than 120 papers published approximately since the second quarter of 2016 till the first quarter of 2018. It is organized according to the particular stacking principles and includes chapters on concentration adjustment (Kohlrausch) stacking, on stacking techniques based on pH changes, on stacking in electrokinetic chromatography and on other stacking techniques. Where available, explicit information is given about the procedure, electrolyte(s) used, detector employed and sensitivity reached. Not reviewed are papers on transient isotachophoresis which are covered by another review in this issue.

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Section: Environmental Analysesmentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

2018

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“…As a result, sample ions could be stacked and concentrated in the boundary between the sample zone and the BGE zone . The CZE‐UV method for the analysis of these five TCs was previously developed in our group . However, the separation voltage was reduced from 25 to 20 KV in order to avoid unstable electric current when applying FASI.…”

Section: Resultsmentioning

confidence: 99%

“…As stated above, a low conductivity sample solvent is necessary in order to have the proper stacking conditions in FASI. Organic solvents of low conductivity or hydro‐organic mixtures have been traditionally used as sample solvents to perform FASI . MeOH, ACN, water and their mixtures in a 50:50 proportion were checked as sample solvents.…”

Section: Resultsmentioning

confidence: 99%

“…CE has gained in popularity in the control of antibiotic residues as an alternative technique to LC due to its clear advantages, such as speed, minimal need of sample, high separation efficiency, reduced consumption of organic solvents, and relatively low running costs . In the last years CE coupled to UV, electrochemiluminescence or MS detection has been applied for the analysis of TCs in pharmaceutical products , food samples , environmental waters and human urine .…”

Section: Introductionmentioning

confidence: 99%

“…Unfortunately, the sensitivity in these methods was not so good due to the short sample plug length that could be injected into the capillary . To improve the sensitivity, large volume sample stacking (LVSS) has also been proposed for determination of TCs in water . Another alternative for preconcentration and simultaneous elimination of some interfering compounds could be the use of field‐amplified sample injection (FASI).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of tetracyclines in human urine samples by capillary electrophoresis in combination with field amplified sample injection

et al. 2017

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A sensitive method using CZE-UV detection has been developed for the determination of five tetracycline antibiotics in human urine samples. To improve the sensitivity of the method, an on-line preconcentration strategy, named field-amplified sample injection, has been developed, based on the electrokinetic injection of the sample, which requires only a 1:100 dilution with sample solvent before injection. Under optimum conditions, sensitivity enhancement factors ranged from 450 to 800 for the studied compounds. The applicability of the proposed method was demonstrated by the determination of these antibiotics in spiked urine samples. The limits of quantification were lower than 0.8 mg/L and the precision (intra- and inter-day), expressed as %RSD was below 14%. Recoveries ranged from 92.1 to 96.7%. Thus, the proposed procedure is a simple, fast and efficient strategy which could be used as therapeutic drug monitoring in human urine samples.

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Large volume sample stacking of cationic tetracycline antibiotics toward 10 ppb level analysis by capillary electrophoresis with UV detection

Huang

et al. 2016

Electrophoresis

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This paper aimed to build up a sensitive CE method for the analysis of tetracyclines (TCs) antibiotics (including tetracycline, chlorotetracycline, oxytetracycline, and doxycycline) with conventional UV detection. Here, the large volume sample stacking was applied to achieve in capillary preconcentration of the targets. To achieve large volume sample stacking, the essential step was a large volume of sample (around 83.3% of total capillary length from inlet to detection window) hydrodynamically loaded. Then, the reserved voltage was added in order to push the sample matrix out of the capillary. Due to different pH between sample solution (pH 4.6) and BGE (pH 11.0), the cationic TCs would turn into negatively charged while the sample matrix was removing from the capillary. Finally, the anionic TCs were stacked at the inlet for the subsequent separation. Although the loss of sample existed during their charge transformation, the LODs could be improved around 40 times than that obtained by normal hydrodynamic injection CE method. Here, the LODs were in the range of 8.1-14.5 μg/L, around 10 ppb that close to the level by electrochemiluminescence or laser-induced fluorescence detection of TCs by CE. The precision was characterized by RSDs of migration times and peak areas, which were in the range of 0.19-0.24% and 0.97-2.54%, respectively. The recoveries of the developed method were in the range of 95-112% by spiking TCs in the tap water. The proposed inline preconcentration CE method could be a simple, speed, and sensitive method for the quantitative analysis of TCs.

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Trace determination of tetracyclines in water samples by capillary zone electrophoresis combining off-line and on-line sample preconcentration

Cited by 30 publications

References 37 publications

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

Determination of tetracyclines in human urine samples by capillary electrophoresis in combination with field amplified sample injection

Large volume sample stacking of cationic tetracycline antibiotics toward 10 ppb level analysis by capillary electrophoresis with UV detection

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