Trace determination of antibacterial pharmaceuticals in fishes by microwave-assisted extraction and solid-phase purification combined with dispersive liquid–liquid microextraction followed by ultra-high performance liquid chromatography-tandem mass spectrometry

Huang, Peiting; Zhao, Peng; Dai, Xinpeng; Hou, Xiaohong; Zhao, Longshan; Liang, Ning

doi:10.1016/j.jchromb.2015.12.059

Cited by 35 publications

(15 citation statements)

References 32 publications

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“…In addition, the mobile phase is also an important factor influencing the ionization efficiency in the ion source and eventually affecting the resolution and sensitivity of the target compounds. The separation effect of acetonitrile and methanol used as the organic phase was compared. The baseline was uneven when acetonitrile was used as the organic phase; therefore, methanol was selected as the organic phase.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of 29 pharmaceuticals in fish muscle and plasma by ultrasonic extraction followed by SPE–UHPLC–MS/MS

Liu

Bao

et al. 2018

J of Separation Science

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A confirmatory method for the simultaneous detection of 29 pharmaceuticals in fish muscle and plasma was developed by using solid-phase extraction combined with ultra-high performance liquid chromatography and tandem mass spectrometry. Fish samples were extracted with methanol and enriched using Oasis HLB solid-phase extraction columns in one step. Twenty-nine target pharmaceuticals were quantified by the internal standard method and the calibration curves showed good linearity in a wide range with determination coefficients of greater than 0.913. The detection limits of the pharmaceuticals ranged from 0.01 to 2.00 μg/kg (μg/L). The applicability of the method was checked by precision and recovery experiments. The average recoveries of the 29 pharmaceuticals were between 61 and 111%, and all the relative standard deviations were below 25%. Our reported method has been demonstrated to be sensitive, convenient, rapid, and reliable for the simultaneous determination of 29 pharmaceuticals in fish muscle and plasma. Real sample determination showed that 25 and 9 of the 29 compounds were detected in fish muscle and plasma, respectively.

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Section: Resultsmentioning

confidence: 99%

Simultaneous determination of 29 pharmaceuticals in fish muscle and plasma by ultrasonic extraction followed by SPE–UHPLC–MS/MS

Liu

Bao

et al. 2018

J of Separation Science

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“…As a demonstration, Nile Blue at 10 −8 m was extracted and concentrated from water to octanol during film evaporation. [42][43][44][45] For microwave-assisted extraction and solid-phase purification combined with DLLME, 200 µL is required. The fluorescence intensity profiles of octanol nanodroplets versus initial volume of Nile Blue solution are shown in Figure 4B.…”

Section: Minimal Volume Of Analyte Solution For the Spinning Ouzo Dromentioning

confidence: 99%

One‐Step Nanoextraction and Ultrafast Microanalysis Based on Nanodroplet Formation in an Evaporating Ternary Liquid Microfilm

Qian

Yamada

Wei

et al. 2019

Adv Materials Technologies

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in chemical analysis workflow is sample pretreatment to extract the analytes before detection. The recent development of dispersive liquid-liquid microextraction (DLLME) has stimulated progress for extracting and preconcentrating trace hydrophobic analytes from aqueous solutions both rapidly and efficiently. [4][5][6] DLLME is based on spontaneous emulsification upon mixing of a water-insoluble extractant, a dispersive solvent, and an analyte aqueous solution. [7,8] The hydrophobic analyte could be rapidly extracted into extractant nanodroplets because of the higher solubility of the analyte in the extractant than in water. The large surface area of nanodroplets enhances the transfer of the analyte from the surrounding solution to the droplets. Relying on the combination of DLLME and various detection techniques, such as inductively coupled plasma optical emission spectrometry or fluorescence spectrometry, trace chemical analysis has been achieved for diverse applications. [9][10][11] However, these existing approaches require significant improvement due to their time-consuming nature, their requirement for specialized equipment in droplet separation and for large volumes of the analyte solution. [12,13] Many studies have focused on concentrating compounds from a single sessile drop evaporation. [14][15][16][17] A common issue comes from the coffee stain effect that leads to uncontrolled deposition of the analyte on the substrate rather than into a focused spot. Although an evaporating drop can shrink isotropically on superhydrophobic surfaces, micro/nanoscale structures on these surfaces can influence solute deposition, in particular at the final stage of evaporation when the drop size becomes comparable with the structures. [18] Supersensitive detection can be achieved for the materials deposited on highly ordered nanostructures on the substrate by using the plasmonic effect. [19] However, these nanostructures are difficult to fabricate for fast and high throughput analysis. Recent work on drop evaporation of ternary liquid mixtures shows advantageous features that may lead to unpinned drop for solute concentrating. [20,21] Selective evaporation of the volatile co-solvent (ethanol) in the mixture leads to oversaturation of oil and consequently spontaneously formation of tiny droplets. This phenomenon of Preconcentration is key for detection from an extremely low concentration solution, but requires separation steps from a large volume of samples using extracting solvents. Here, a simple approach is presented for ultrafast and sensitive microanalysis from a tiny volume of aqueous solutions. In this approach, liquid-liquid nanoextraction in an evaporating thin liquid film on a spinning substrate is coupled with quantitative analysis in one step. The approach is exemplified using a liquid mixture comprising a target compound to be analyzed in water, mixed with extractant oil and co-solvent ethanol. With rapid evaporation of ethanol, nanodroplets of oil form spontaneously in the film. The compounds are highly conce...

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“…When analytes are captured in or adsorbed onto for example soil, manure, food, plant cells or biological tissues, the analytes first need to be extracted from the solid material. Therefore, ultrasound or microwave-assisted extraction can be used prior to an additional enrichment step such as LLE, SPE or SPME [80,[126][127][128]. Another option is to use Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction salts.…”

Section: (D) Sample Preparationmentioning

confidence: 99%

Quantitative mass spectrometry methods for pharmaceutical analysis

Loos¹,

Schepdael²,

Cabooter³

2016

Phil. Trans. R. Soc. A.

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Quantitative pharmaceutical analysis is nowadays frequently executed using mass spectrometry. Electrospray ionization coupled to a (hybrid) triple quadrupole mass spectrometer is generally used in combination with solid-phase extraction and liquid chromatography. Furthermore, isotopically labelled standards are often used to correct for ion suppression. The challenges in producing sensitive but reliable quantitative data depend on the instrumentation, sample preparation and hyphenated techniques. In this contribution, different approaches to enhance the ionization efficiencies using modified source geometries and improved ion guidance are provided. Furthermore, possibilities to minimize, assess and correct for matrix interferences caused by co-eluting substances are described. With the focus on pharmaceuticals in the environment and bioanalysis, different separation techniques, trends in liquid chromatography and sample preparation methods to minimize matrix effects and increase sensitivity are discussed. Although highly sensitive methods are generally aimed for to provide automated multi-residue analysis, (less sensitive) miniaturized set-ups have a great potential due to their ability for in-field usage. This article is part of the themed issue ‘Quantitative mass spectrometry’.

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Trace determination of antibacterial pharmaceuticals in fishes by microwave-assisted extraction and solid-phase purification combined with dispersive liquid–liquid microextraction followed by ultra-high performance liquid chromatography-tandem mass spectrometry

Cited by 35 publications

References 32 publications

Simultaneous determination of 29 pharmaceuticals in fish muscle and plasma by ultrasonic extraction followed by SPE–UHPLC–MS/MS

Simultaneous determination of 29 pharmaceuticals in fish muscle and plasma by ultrasonic extraction followed by SPE–UHPLC–MS/MS

One‐Step Nanoextraction and Ultrafast Microanalysis Based on Nanodroplet Formation in an Evaporating Ternary Liquid Microfilm

Quantitative mass spectrometry methods for pharmaceutical analysis

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