Simultaneous determination of 29 pharmaceuticals in fish muscle and plasma by ultrasonic extraction followed by SPE–UHPLC–MS/MS

Abstract

A confirmatory method for the simultaneous detection of 29 pharmaceuticals in fish muscle and plasma was developed by using solid-phase extraction combined with ultra-high performance liquid chromatography and tandem mass spectrometry. Fish samples were extracted with methanol and enriched using Oasis HLB solid-phase extraction columns in one step. Twenty-nine target pharmaceuticals were quantified by the internal standard method and the calibration curves showed good linearity in a wide range with determination coefficients of greater than 0.913. The detection limits of the pharmaceuticals ranged from 0.01 to 2.00 μg/kg (μg/L). The applicability of the method was checked by precision and recovery experiments. The average recoveries of the 29 pharmaceuticals were between 61 and 111%, and all the relative standard deviations were below 25%. Our reported method has been demonstrated to be sensitive, convenient, rapid, and reliable for the simultaneous determination of 29 pharmaceuticals in fish muscle and plasma. Real sample determination showed that 25 and 9 of the 29 compounds were detected in fish muscle and plasma, respectively.

MIL-101(Fe)@TiO2 nanotube composite material is used for the solid phase extraction of non-steroidal anti-inflammatory drugs under the synergy of multiple interactions

“…This is mainly due to the large number of adsorption sites of the sorbent and its various interactions with the analyte. Although a lower LOD (0.01–2 μg L −1 ) was obtained by the SPME-HPLC-UV and SPE-UHPLC-UV methods, 32,35 they require more sorbents, expensive processing equipment and cumbersome fiber preparation steps. However, the established method also has some limitations, including a longer extraction time.…”

Analysis of pharmaceuticals in fish using ultrasound extraction and dispersive spe clean-up on que Z-Sep/C18 followed by LC-QToF-MS detection

“…The validated method is comparable to other previously reported methods in which PhACs are extracted by USE in the fish muscle ( Table 3 ). In most cases a clean-up with SPE is performed [20 , [31] , [32] , [33] , [34] . However, these methods take into account less than 30 PhACs with recoveries ranging from slightly more than 30 to 118%, and the determination of which was developed using low resolution triple quadrupole mass spectrometry (QqQ) instruments.…”

“… 0.03 – 5.5 40 - 160 Q-Exactive 54 - (−327) [35] 1.0 dw QuEChERS 19 EMR lipid removal 21 0.5– 91 DW 1.8 - 303 dw 10 - 139. Q-ToF 113 - (−89) [1] 0.2 dw vortex 10 filtration 42 0.01 to 2.00 0.1 - 40.2 29 - 188 QqQ 2191 -(−83) [36] 1 USE NR SPE 29 0.01–2.00 0.03 - 6.67 61 - 111 QqQ NR [32] 5 USE-McIlvain 15 SPE 27 N.R. 6–30 43 - 103 QqQ 5 - (−40) [33] 1 z.f.…”

Seasonal Occurrence of Ibuprofen in Sediment, Water, and Biota in River Owena and Ogbese, and its Ecological Risk Assessment

“…100 mL of the sample was transferred into the cartridges and washed with 5% v/v methanol in water. Cartridges were eluted with 3 mL x 2 methanol and subsequently evaporated using N2 and reconstituted with 0.5 mL of methanol before it was transferred to vials for HP-LC analysis using methods as described by Liu et al, (2018) and Huerta et al, (2013).…”