Trace determination of 10 β-lactam antibiotics in environmental and food samples by capillary liquid chromatography

Bailón-Pérez, M. Isabel; Garcı́a-Campaña, Ana M.; Olmo‐Iruela, Monsalud del; Gámiz‐Gracia, Laura; Cruces‐Blanco, Carmen

doi:10.1016/j.chroma.2009.09.042

Cited by 55 publications

(25 citation statements)

References 29 publications

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“…Traditionally, capillary HPLC uses fused silica capillaries prepared with a variety of different stationary phases; these phases seem to be very promising in separating a wide variety of analytes in different application fields, including pharmaceutical, clinical, environmental, agrochemical and biological samples [4][5][6][7][8][9]. However, the successful development of these techniques is closely related to the technical challenges associated with the column manufacturing.…”

Section: Introductionmentioning

confidence: 99%

“…Methacrylates are now one of the most widely popular monoliths used as chromatographic separation media [21,22]. There are several advantages associated with using methacrylate-based polymers as monolithic stationary phases, including high stability in a wide range of mobile phase pH (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12), fast and simple preparation and easy functionalization. The methacrylate monolithic columns have also various selectivities towards monomers with wide ranging polarities [23,24].…”

Section: Introductionmentioning

confidence: 99%

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Preparation and Evaluation of Long Chain Alkyl Methacrylate Monoliths for Capillary Chromatography

et al. 2011

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This work describes the fabrication of long chain alkyl methacrylate monolithic materials for use as stationary phases in capillary liquid chromatography. After capillary inner wall surface activation with 3-(trimethoxysilyl)propyl methacrylate, monoliths were formed by copolymerization of either lauryl or stearyl methacrylate (LMA or SMA) with ethylene dimethacrylate (EDMA) as crosslinker, in the presence of azobisisobutyronitrile (AIBN) as initiator and a mixture of porogenic solvents including water, 1-propanol and 1,4-butanediol. The composition of the polymerization mixture was changed in terms of monomer, crosslinker and porogen ratio composition, in order to compare the influence of these parameters. The monoliths were prepared in 320 lm i.d. and 200 mm length capillaries. The column morphology was characterized by optical microscopy and scanning electron microscopy (SEM). Total porosity and permeability of each column were calculated using uracil as unretained material by measuring the pressure drop across the columns as a function of linear velocity. The microglobule average size for each column was also determined using Hagen-Poiseuille equation and compared with the SEM images. As expected, a decrease of the porogen to monomer ratio corresponded to smaller microglobules and a lower total porosity. The columns were then chromatographically evaluated; good results were obtained when these capillaries were used to separate mixtures of phenols, aromatics and drug compounds.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Long Chain Alkyl Methacrylate Monoliths for Capillary Chromatography

et al. 2011

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“…In milk, the most commonly used methodologies are liquid chromatography (LC) (Bailon-Perez, Garcia-Campana, del Olmo, & Iruela Cruces-Blanco, 2009) gas chromatography (GC) (Galbusera & Chen, 2003;Le, Wan, & Lam, 2002), high performance liquid chromatography (HPLC) (Dasenbrock & La Course, 1998) and mass spectrometry (MS) (Xu et al, 2010). Unfortunately, these methods present some limitations: they are time-consuming, require complex sample preparation and the use of expensive equipment.…”

Section: Introductionmentioning

confidence: 99%

A novel fluorescence polarization assay for determination of penicillin G in milk

et al. 2016

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“…Nevertheless, CE is frequently used in the quantitative and qualitative determinations of different β-lactam antibiotics being considered today a useful alternative and also a complementary technique to the more frequently used HPLC methods (Garcia-Ruiz, Marina, 2006;Baillon-Perez et al, 2009;Garcia-Campana et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of amoxicillin and clavulanic acid in pharmaceutical preparations by capillary zone electrophoresis

Hancu

Neacşu

Papp

et al. 2016

Braz. J. Pharm. Sci.

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Clavulanic acid enhances the antibacterial spectrum of amoxicillin by rendering most β-lactamase producing isolates susceptible to the drug. A fast, simple and efficient capillary electrophoresis method was developed for the simultaneous determination of amoxicillin and clavulanic acid from complex mixtures. Using a 25 mM sodium tetraborate as background electrolyte at a pH of 9.30, + 25 kV applied voltage, 25 °C system temperature, UV determination at 230 nm; we succeeded in simultaneous separation of amoxicillin and clavulanic acid in approximately 2 minutes. The analytical performance of the method was evaluated in terms of reproducibility, precision, accuracy, and linearity. The optimized analytical method was applied for the determination of the two analytes from combined commercial pharmaceutical preparations. This CE method is fast, inexpensive, efficient, and environmentally friendly when compared with the more frequently used high performance liquid chromatography methods described in the literature. O ácido clavulânico acentua o espectro antibacteriano de amoxicilina, tornando a maioria dos isolados produtores de β-lactamase sensíveis ao fármaco. Desenvolveu-se um método rápido, simples e eficiente de electroforese capilar (EC) para a determinação simultânea de amoxicilina e de ácido clavulânico a partir de misturas complexas. Usando tetraborato de sódio 25 mM como electrólito em pH de 9,30, voltagem aplicada de + 25 kV, em sistema a 25 ° C e determinação por UV a 230 nm, a foi bem-sucedida a separação simultânea de amoxicilina e ácido clavulânico em, aproximadamente, 2 minutos. O desempenho analítico do método foi avaliado em termos de reprodutibilidade, precisão, exatidão e linearidade. O método analítico otimizado foi aplicado para a determinação dos dois analitos em associação, a partir de preparações farmacêuticas comerciais. Este método de EC é rápido, barato, eficiente e ecologicamente correto, quando comparado aos métodos de cromatografia líquida de alta eficiência mais frequentemente descritos na literatura.Unitermos: Amoxicilina/determinação. Acido clavulânico/determinação. Eletroforese capilar/análise quantitativa. Antibacterianos/análise quantitativa.

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Trace determination of 10 β-lactam antibiotics in environmental and food samples by capillary liquid chromatography

Cited by 55 publications

References 29 publications

Preparation and Evaluation of Long Chain Alkyl Methacrylate Monoliths for Capillary Chromatography

Preparation and Evaluation of Long Chain Alkyl Methacrylate Monoliths for Capillary Chromatography

A novel fluorescence polarization assay for determination of penicillin G in milk

Simultaneous determination of amoxicillin and clavulanic acid in pharmaceutical preparations by capillary zone electrophoresis

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