A novel fluorescence polarization assay for determination of penicillin G in milk

Pennacchio, Anna; Varriale, Antonio; Scala, Andrea; Marzullo, Vincenzo Manuel; Staiano, Maria; D’Auria, Sabato

doi:10.1016/j.foodchem.2015.05.127

Cited by 44 publications

(16 citation statements)

References 20 publications

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“…The results obtained suggest that the method could be applied directly in milk without interference. For the PenG detection, the LoD of the method was 1.0 nmol/L, which is much less than the required maximum residual limit (MRL) in EU regulations (12.0 nmol/L) [134] while the obtained results for the ciprofloxacin reached a sensitivity of 1 ppb [135], 100 times lower than MRL of ciprofloxacin in milk, as fixed by the European Union regulation (100 ppb).…”

Section: Foodmentioning

confidence: 72%

“…A microarray of different spots was printed on a modified glass chip. Recently, it has been developed a fluorescence polarization method to detect directly in milk the presence of PenG [134] and Ciprofloxacin [135]. This methodology is based on the increase of fluorescence polarization emission of a fluorescence-labeled compound derivative upon their binding to specific antibodies.…”

Section: Foodmentioning

confidence: 99%

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Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

Camarca

Varriale

Capo

et al. 2021

Sensors

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The purpose of this work is to provide an exhaustive overview of the emerging biosensor technologies for the detection of analytes of interest for food, environment, security, and health. Over the years, biosensors have acquired increasing importance in a wide range of applications due to synergistic studies of various scientific disciplines, determining their great commercial potential and revealing how nanotechnology and biotechnology can be strictly connected. In the present scenario, biosensors have increased their detection limit and sensitivity unthinkable until a few years ago. The most widely used biosensors are optical-based devices such as surface plasmon resonance (SPR)-based biosensors and fluorescence-based biosensors. Here, we will review them by highlighting how the progress in their design and development could impact our daily life.

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Section: Foodmentioning

confidence: 72%

Section: Foodmentioning

confidence: 99%

Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

Camarca

Varriale

Capo

et al. 2021

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…FPIA, a homogeneous analysis technique, is mainly used for the rapid monitoring of small molecular compounds (Dong et al, 2019;Pennacchio et al, 2016;Wang et al, 2019), and this has been developed to detect Pb(II) and Cd(II) (Johnson, 1999;Johnson et al, 2002). In these studies, a fluorescent analogue of the metal-chelate complex (termed a tracer) was constructed to regulate the fluorescence polarization value through the transformation of the bound and unbound state to antibody molecules.…”

Section: Fluorescence Polarization Immunoassay (Fpia)mentioning

confidence: 99%

Antibody developments for metal ions and their applications

Wang

Zhang

Liu

2020

Food and Agricultural Immunology

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Metal ions are beneficial or toxic in part depending on their identity, valence state, and concentration. Antibodies against radioactive metal ions can be used in medical diagnosis and therapy, while antibodies against heavy metal ions are widely used in the trace element analysis of the immunoassay. This review focuses on the recent advances in antibody developments for metal ions and their applications. We exhaustively enumerated 18 chelate or ligand structures for metal hapten synthesis, which were proven to produce antibodies that cover almost 33 metal ions. The binding properties of these antibodies for their corresponding haptens were also discussed both in the structural foundation and molecular foundation. Furthermore, the representative applications of the antibodies used in medical diagnostics and therapy, and immunoassays were summarized. Finally, we illustrated the present challenges in high specific metal ion antibody preparation, and in immunoassay development. The future perspectives were also discussed.

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“…These methods have different restrictions that make them challenging to apply outside the laboratory. Therefore, there is a growing need for quick, easy, and not expensive methods to detect and manage ciprofloxacin residues in food matrices [15][16][17][18] .Fluorescence polarization assays represent a valid alternative to detect chemical contaminants in food and milk samples 19 . In this work, a fluorescence polarization assay for the detection of the presence of ciprofloxacin in milk was developed using ad hoc synthesized fluorescence ciprofloxacin-conjugate (GlnBP-CPFX), covalently labeled with a near-infrared (NIR) fluorescence dye, and commercial monoclonal anti-ciprofloxacin antibody.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Fluorescence polarization assay to detect the presence of traces of ciprofloxacin

Kojok

Darra

Khalil

et al. 2020

Sci Rep

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Detection of ciprofloxacin residues in milk by sensitive and rapid methods is of great interest due to its use in the treatment of dairy livestock health. Current analytical approaches to antibiotics detection, are laboratory-based methods and they are time-consuming and require trained personnel. To cope this problem, we propose an assay, based on fluorescence polarization principle, able to detect the presence of ciprofloxacin in diluted milk sample without any pre-treatment. The proposed method is based on the use of ciprofloxacin-protein conjugate labeled with near infrared fluorescence dye, which upon binding to specific antibody causes an increase of the fluorescence polarization emission signal. The developed assay allows for the detection of ciprofloxacin at a concentration of 1ppb, which represents an amount lower than the maximum residual limit (MRL) of ciprofloxacin in milk, as set by the European Union regulation (100 ppb).The antibiotics treatment plays a crucial role in dairy livestock health 1 . It is being used for therapeutic purposes as well as for improving breeding efficiency. However, this treatment can leave residues of antibiotics in foods of animal origin (milk and meat), and consequently an increase of the human exposure to antibiotics 2 . While assessing the usage of antibiotic residues, the exceptional use of such drugs was legally considered 3 . Hence, the risk of the presence of antibiotic residues in milk and dairy products could increase in case that a required elimination period of them in food is not properly defined.Antibiotic residues in milk and dairy products present detrimental consequences on the consumer health, causing disturbances in the intestinal flora as well as allergic reactions that could lead to anaphylaxis 4 . Moreover, antibiotic residues might increase microbial resistance, a major health risk 5 . Among anti-bacterial agents used for the management of infections in dairy livestock, ciprofloxacin, belonging to the fluoroquinolone class, is the common antibiotic used in case of pulmonary, urinary and digestive infections 6 . However, ciprofloxacin could cause hypersensitivity in humans 7 . Due to the importance of considering the presence of ciprofloxacin in milk, a residue surveillance of anti-microbial presence is crucial to support food-farming practices, upraising consequently the food safety level. It is to be noted that the maximum residual limit (MRL) for ciprofloxacin in milk has been fixed at 100 ppb (Council Regulation EEC/2377/90). In Lebanon, few studies have investigated the presence of antibiotic residues in milk [8][9][10] . A recent study showed that ciprofloxacin and oxytetracycline are mostly found antibiotics in milk in Lebanon (personal communication).Currently, laboratory-based methods, such as liquid chromatography-mass spectrometry (LC-MS), high performance liquid chromatography (HPLC) [11][12][13] and microbiological assays 14 are the common used approaches for ciprofloxacin detection. These methods have different restrictions that make the...

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A novel fluorescence polarization assay for determination of penicillin G in milk

Cited by 44 publications

References 20 publications

Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

Antibody developments for metal ions and their applications

Fluorescence polarization assay to detect the presence of traces of ciprofloxacin

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