Trabecular bone structural variation in biopsy sites of the beagle ilium

Abstract

Iliac trabecular bone sites were studied in 2 male and 2 female adult beagles to determine normal structural variability among different sites, and to identify transilial biopsy sites with minimum structural variation. Only sites that provided adequate amounts of trabecular bone were studied. Trabecular bone area and perimeter were measured on microradiographs of ground sagittal sections using a Quantimet 720 image analyzer, and percent bone, perimeter to area ratio, and mean trabecular width were calculated. Over 100% variation was found in the parameters studies among sections from different sites within the same animal. However, variability was minimal among sections obtained more than 2 mm from the lateral or medial cortex within the most caudal sites evaluated. Two sites appropriate for sampling with an 8 mm trephine were identified as being centered over the following points: (a) a point 7 mm caudal to the cranial dorsal spine and 7 mm ventral to the tuber sacrale, and (b) a point 7 mm caudal to the cranial ventral spine and 7 mm dorsal to the tuber coxae. Comparison of data obtained from males and females indicated that sampling variation may also be reduced by using dogs of one sex and by preferential use of males.

Distribution of mineralization indices of modeling and remodeling over eight months in middiaphyseal cross sections of femurs from adult swine

“…Histological endpoints associated with bone modeling and remodeling 1 vary among and within bones (Amling et al, 1996;Amprino and Marotti, 1964;Harris et al, 1968;Lee et al, 1965;Marotti, 1976;Martin et al, 1980;Soni, 1968;Wronski et al, 1981). For single bones, the range of inter-and intra-individual variation is most extensively documented for specific ribs (Frost, 1969;Tommerup et al, 1991) and the ilium (de Vernejoul et al, 1981;Ellis and Peart, 1972;Hardt and Jee, 1982;Malluche et al, 1982;Melson et al, 1978;Savage et al, 1991;Whitehouse, 1977) due to past (ribs) or current (ilium) application in diagnostic clinical evaluation (Bordier and Tun Chot, 1972;Melson and Mosekilde, 1981;Recker, 1994;Santoro and Frost, 1967). Quantitative documentation of normal histological variation in long bone transverse sections is rather limited (Martin et al, 1980;Raab et al, 1991), even though long bones are used commonly in assessments of bone response to mechanical loading (Hert et al, 1972;O'Connor et al, 1982;Raab et al, 1991).…”

Porous hydroxyapatite as a bone graft substitute in diaphyseal defects: A histometric study

“…The implant specimens were composed of (mean 2 SE) 10.6% 2 1.0% soft tissue, 51.2% t 1.3% bone, and 38.2% 2 1.0% IP200. The graft specimens showed good union with little apparent ingrowth at 3 months, followed by progressive appositional closure of cancellous spaces.…”

Short‐term oral phosphate and bone remodeling in beagles

“…Pharmaceuticals, Cambridge, Ontario). A 2 cm x 0.6 cm cranial dorsal portion of the right iliac crest (Hardt and Jee, 1982) and a 2 cm midshaft segment of right seventh rib were removed from each dog using a bone cutter during the anesthesia. The specimens were fixed in 70% ethanol immediately.…”