TPX2 Protein of Arabidopsis Activates Aurora Kinase 1, But Not Aurora Kinase 3 In Vitro

Tomaštíková, Eva Dvořák; Demidov, Dmitri; Jeřábková, Hana; Binarová, Pavla; Houben, Andreas; Doležel, Jaroslav; Petrovská, Beáta

doi:10.1007/s11105-015-0890-x

Cited by 18 publications

(27 citation statements)

References 29 publications

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“…However, to date, only histone H3 (Hsu et al, 2000) and TPX2 have been reported as alpha Aurora substrates in plants (Demidov et al, 2005;Tomaštíková et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Namely, the double alpha Aurora knockdown mutant aur1/aur2 exhibits defects in orienting formative cell divisions (Van Damme et al, 2011), while RNAi silencing displays impaired cytokinesis with defective cell plate formation (Petrovská et al, 2012). The preferential phosphorylation of the microtubule-associated protein TPX2 by Arabidopsis Aurora 1 (Tomaštíková et al, 2015) and the colocalization of TPX2 and Aurora 1 on MTs (Petrovská et al, 2012) hint toward a role of alpha Aurora kinases in the regulation of cytoskeletal dynamics.…”

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confidence: 99%

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Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression

et al. 2016

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“…However, to date, only histone H3 (Hsu et al, 2000) and TPX2 have been reported as alpha Aurora substrates in plants (Demidov et al, 2005;Tomaštíková et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression

et al. 2016

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“…Binding of TPX2 to human Aurora A activates the phosphorylation activity of the kinase and protects it from dephosphorylation by protein phosphatase 2A [6]. Similarly, Aurora kinase binding domain of canonical Arabidopsis TPX2 can activate Aurora1 in vitro [15]. Recently we showed that Aurora kinase binding domains of TPXL2 and TPLX3 also can activate Aurora1 in vitro [18].…”

Section: Functional Prediction Of Arabidopsis Aurora Binding Domainmentioning

confidence: 97%

“…Further, an additional microtubule-binding motif is present at the N-terminal part of plant TPX2 protein [14]. Arabidopsis TPX2 was described as an activator of Aurora1 in vitro [15]. In planta, Aurora1 colocalizes with TPX2 at spindle microtubules throughout mitosis and co-precipitates with TPX2 on microtubules in a cell cycle-specific manner [16].…”

Section: Introductionmentioning

confidence: 99%

Functional Divergence of Microtubule-Associated TPX2 Family Members in Arabidopsis thaliana

Tomaštíková

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Dvořák

et al. 2020

IJMS

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TPX2 (Targeting Protein for Xklp2) is an evolutionary conserved microtubule-associated protein important for microtubule nucleation and mitotic spindle assembly. The protein was described as an activator of the mitotic kinase Aurora A in humans and the Arabidopsis AURORA1 (AUR1) kinase. In contrast to animal genomes that encode only one TPX2 gene, higher plant genomes encode a family with several TPX2-LIKE gene members (TPXL). TPXL genes of Arabidopsis can be divided into two groups. Group A proteins (TPXL2, 3, 4, and 8) contain Aurora binding and TPX2_importin domains, while group B proteins (TPXL1, 5, 6, and 7) harbor an Xklp2 domain. Canonical TPX2 contains all the above-mentioned domains. We confirmed using in vitro kinase assays that the group A proteins contain a functional Aurora kinase binding domain. Transient expression of Arabidopsis TPX2-like proteins in Nicotiana benthamiana revealed preferential localization to microtubules and nuclei. Co-expression of AUR1 together with TPX2-like proteins changed the localization of AUR1, indicating that these proteins serve as targeting factors for Aurora kinases. Taken together, we visualize the various localizations of the TPX2-LIKE family in Arabidopsis as a proxy to their functional divergence and provide evidence of their role in the targeted regulation of AUR1 kinase activity.

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“…1b). Peaks denoted 8C and 16C are the result of endoreduplication that could be due to rapid cell cycling (Pathirana and Eason 2006) or a defect in the expression of Aurora kinases (Petrovska et al 2012;Tomastikova et al 2016). Twelve hours after release from the block, two peaks of G1 (2C) and G2 (4C) were visible with more 4C and 8C nuclei than 2C (Fig.…”

Section: Cell Cycle Synchronisation and The Timing Of Cell Death Indumentioning

confidence: 99%

Cell death patterns in Arabidopsis cells subjected to four physiological stressors indicate multiple signalling pathways and cell cycle phase specificity

et al. 2016

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Corpse morphology, nuclear DNA fragmentation, expression of senescence-associated genes (SAG) and cysteine protease profiles were investigated to understand cell death patterns in a cell cycle-synchronised Arabidopsis thaliana cell suspension culture treated with four physiological stressors in the late G2 phase. Within 4 h of treatment, polyethylene glycol (PEG, 20 %), mannose (100 mM) and hydrogen peroxide (2 mM) caused DNA fragmentation coinciding with cell permeability to Evans Blue (EB) and produced corpse morphology corresponding to apoptosis-like programmed cell death (AL-PCD) with cytoplasmic retraction from the cell wall. Ethylene (8 mL per 250-mL flask) caused permeability of cells to EB without concomitant nuclear DNA fragmentation and cytoplasmic retraction, suggesting necrotic cell death. Mannose inducing glycolysis block and PEG causing dehydration resulted in relatively similar patterns of upregulation of SAG suggesting similar cell death signalling pathways for these two stress factors, whereas hydrogen peroxide caused unique patterns indicating an alternate pathway for cell death induced by oxidative stress. Ethylene did not cause appreciable changes in SAG expression, confirming necrotic cell death. Expression of AtDAD, BoMT1 and AtSAG2 genes, previously shown to be associated with plant senescence, also changed rapidly during AL-PCD in cultured cells. The profiles of nine distinct cysteine protease-active bands ranging in size from ca. 21.5 to 38.5 kDa found in the control cultures were also altered after treatment with the four stressors, with mannose and PEG again producing similar patterns. Results also suggest that cysteine proteases may have a role in necrotic cell death.

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TPX2 Protein of Arabidopsis Activates Aurora Kinase 1, But Not Aurora Kinase 3 In Vitro

Cited by 18 publications

References 29 publications

Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression

Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression

Functional Divergence of Microtubule-Associated TPX2 Family Members in Arabidopsis thaliana

Cell death patterns in Arabidopsis cells subjected to four physiological stressors indicate multiple signalling pathways and cell cycle phase specificity

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