Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression

Boruc, Joanna; Weimer, Annika K.; Stoppin‐Mellet, Virginie; Mylle, Evelien; Kosetsu, Ken; Cedeño, Cesyen; Jaquinod, Michel; Njo, Maria; Milde, Liesbeth De; Tompa, Péter; González, Nathalie; Inzé, Dirk; Beeckman, Tom; Vantard, Marylin; Damme, Daniël Van

doi:10.1104/pp.16.01602

Cited by 46 publications

(52 citation statements)

References 96 publications

(127 reference statements)

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“…Expression of interactors was confirmed by immuno-blotting (1-589) in wild type localizes to the phragmoplast midzone (Fig 4). Interestingly, GFP-POK2 (2,083-2,771) only colocalizes with MAP65-1-RFP along microtubules, but it is not recruited to the cytosol by MAP65-1(9D) mutant that fails to bind microtubules in tobacco (Fig EV3D and E) [21], suggesting that interaction of POK2 and MAP65 requires the presence of microtubules. Arrow indicates a characteristic gap in the incomplete cell plate.…”

Section: Pok2 Interacts With Map65-3/ple Via Two Distinct Binding Sitesmentioning

confidence: 99%

“…GFP-POK2 (1-2,771): pENTR:POK2 (1-2,771) was recombined with pFK241 pGreenIIS in a Gateway LR reaction. MAP65-X-RFP: pENTR:MAP65-3/PLE, pDONR207: MAP65-1 [16], pDONR207: MAP65-1-(9D) [21] and pDONR207: MAP65-5 [16] were used in a Gateway LR reaction (Invitrogen, 11791-020) with pB7RWG2 to create the C-terminal RFP-fusion proteins [59].…”

Section: Generating Entry Clonesmentioning

confidence: 99%

“…Several other members of the MAP65 family act redundantly with MAP65-3/PLE and localize at the entire phragmoplast or its midzone [15][16][17][18]. Aurora kinase and mitogen-activated protein kinases (MAPK) deactivate MAP65 and release the anti-parallel microtubule overlap [18,19,21]. Aurora kinase and mitogen-activated protein kinases (MAPK) deactivate MAP65 and release the anti-parallel microtubule overlap [18,19,21].…”

Section: Introductionmentioning

confidence: 99%

“…Spatial confinement to the phragmoplast midzone and MAP65 activity during cytokinesis is regulated by phosphorylation [19,20]. Aurora kinase and mitogen-activated protein kinases (MAPK) deactivate MAP65 and release the anti-parallel microtubule overlap [18,19,21].…”

Section: Introductionmentioning

confidence: 99%

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Dual localized kinesin‐12 POK 2 plays multiple roles during cell division and interacts with MAP 65‐3

et al. 2018

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Kinesins are versatile nano-machines that utilize variable non-motor domains to tune specific motor microtubule encounters. During plant cytokinesis, the kinesin-12 orthologs, PHRAGMOPLAST ORIENTING KINESIN (POK)1 and POK2, are essential for rapid centrifugal expansion of the cytokinetic apparatus, the phragmoplast, toward a pre-selected cell plate fusion site at the cell cortex. Here, we report on the spatio-temporal localization pattern of POK2, mediated by distinct protein domains. Functional dissection of POK2 domains revealed the association of POK2 with the site of the future cell division plane and with the phragmoplast during cytokinesis. Accumulation of POK2 at the phragmoplast midzone depends on its functional POK2 motor domain and is fine-tuned by its carboxy-terminal region that also directs POK2 to the division site. Furthermore, POK2 likely stabilizes the phragmoplast midzone via interaction with the conserved microtubule-associated protein MAP65-3/PLEIADE, a well-established microtubule cross-linker. Collectively, our results suggest that dual localized POK2 plays multiple roles during plant cell division.

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Section: Pok2 Interacts With Map65-3/ple Via Two Distinct Binding Sitesmentioning

confidence: 99%

Section: Generating Entry Clonesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dual localized kinesin‐12 POK 2 plays multiple roles during cell division and interacts with MAP 65‐3

et al. 2018

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“…An aur1/aur2 double mutant shows defects in division plane orientation mainly during formative cell division in embryogenesis and early stages of lateral root development, suggesting α Aurora's critical function in establishing cellular asymmetry . Aurora kinases have also been implicated in mitotic and meiotic chromosome segregation in plants (Demidov et al, 2014;Kurihara et al, 2006;Niu et al, 2015) and in securing efficient cell cycle progression through phosphorylation of the MT-bundling protein MAP65-1 (Boruc et al, 2017).…”

mentioning

confidence: 99%

The TPX-Like protein TPXL3, but not TPX2, is the primary activator of α Aurora kinases and is essential for embryogenesis in Arabidopsis

Boruc

Deng

Mylle

et al. 2018

Preprint

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Aurora kinases are key regulators of mitosis. Multicellular eukaryotes generally possess two functionally diverged types. In plants like Arabidopsis, these are termed α versus β Auroras. As the functional specification of Aurora kinases is determined by their specific interaction partners, we initiated interactomics analyses using both α Aurora kinases (AUR1 and AUR2). Proteomics results revealed the TPX2-Like proteins 2 and 3 (TPXL2/3) prominently associating with α Auroras, as did the conserved TPX2 to a lower degree. Like TPX2, TPXL2 and TPXL3 strongly activated AUR1 kinase but exhibited cell cycle-dependent localization differences on microtubule arrays. The separate functions of TPX2 and TPXL2/3 were also suggested by their different influences on AUR1 localization upon ectopic expressions. Furthermore, genetic analyses disclosed that TPXL3, but not TPX2 and TPXL2, acts non-redundantly to secure proper embryo development. In contrast to vertebrates, plants expanded the TPX2 family for both redundant and unique functions among its members.

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Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy

Hoermayer

Friml

Glanc

2021

Methods in Molecular Biology

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Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression

Cited by 46 publications

References 96 publications

Dual localized kinesin‐12 POK 2 plays multiple roles during cell division and interacts with MAP 65‐3

Dual localized kinesin‐12 POK 2 plays multiple roles during cell division and interacts with MAP 65‐3

The TPX-Like protein TPXL3, but not TPX2, is the primary activator of α Aurora kinases and is essential for embryogenesis in Arabidopsis

Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy

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