Toxoplasma gondii genotyping from free-range chickens (Gallus gallus domesticus) in a rural area of Rio Grande do Sul, Brazil

Camillo, Giovana; Machado, M. E. A.; Cadore, Gustavo Cauduro; Bräunig, Patrícia; Venturini, Marı́a Cecilia; Pardini, Lais Luján; Barros, Luiz Daniel de; Garcia, João Luís; Sangioni, Luís Antônio; Vogel, Fernanda Silveira Flôres

doi:10.1590/1678-4162-11732

Cited by 4 publications

(1 citation statement)

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“…For this reason, chickens are often used in research in order to isolate the parasite for epidemiological and genetic studies (Alizadeh-Sarabi et al, 2020;Fernandes et al, 2016;Geuthner et al, 2019;Khan et al, 2020;Mahami-Oskouei et al, 2017). Thus, genotyping studies of T. gondii isolates in free-range chickens have been conducted all over the world and have contributed to the expansion of available epidemiological information on the genetic diversity of this parasite (Moré et al, 2012;Tilahun et al, 2013;Rodrigues et al, 2019;Camillo et al, 2020;Zrelli et al, 2022;Ali Awad et al, 2023) Studies have shown a high prevalence of T. gondii infection in birds in Brazil's south (Camillo et al, 2015) and southeast regions (Lopes et al, 2016), suggesting high environmental contamination. An evaluation of the genotypic characteristics of T. gondii in free-range chickens in the metropolitan area of Goiânia, state of Goiás by Rezende et al (2021) revealed that 96% of tested chickens showed seropositivity for anti-T. gondii, and 64% of tissue samples contained the parasite's DNA.…”

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confidence: 99%

Molecular characterization and epidemiology of Toxoplasma gondii isolates from free-range chickens in the southwest region of Goiás: new genotypes

Domann,

Rezende,

Fleury

et al. 2023

Rev. Bras. Parasitol. Vet.

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The purpose of this study was to isolate Toxoplasma gondii from tissues of free-range chickens in the southwestern region of Goiás, to detect and molecularly characterize the genetic material of the parasite, and to determine the seroprevalence of the protozoan parasite in these animals. A seroprevalence of T. gondii antibodies of 76% (19/25) was found among the chickens, while genetic material from their tissues was detected in 56% (14/25). A total of 14 isolates was obtained in the bioassay, ten of which were considered acute, eight were considered isolates of high virulence lethal to mice, and four of low virulence, considered non-lethal but with the ability to chronify the infection. Seven of the ten isolates showed significant morphometric differences from the RH strain, in terms of nucleus-complex-apical distance, length and width. Genotyping of the acute isolates was performed by RFLP-PCR, using 11 genetic markers: SAG1, SAG2 (3’SAG2 and 5’SAG2), alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and APICO. The results were compared and classified according to the genotypes listed on the ToxoDB Platform, where different profiles were observed indicating the presence of two known genotypes (#7 and #63) and five new genotypes (NEW 3, NEW4, NEW5, NEW6, NEW 7). The results showed high seroprevalence, isolation rate, molecular detection and genotypic variations of T. gondii in free-range chickens in the southwestern region of Goiás.

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