Toxicopathic changes and genotoxic effects in liver of rat following exposure to diazinon

Ezzi, Lobna; Haouas, Zohra; Salah, Imen Belhadj; Sakly, Amina; Grissa, Intissar; Chakroun, Sana; Kerkeni, Emna; Hassine, Mohsen; Mehdi, Meriem; Cheikh, Hassen Ben

doi:10.1007/s11356-016-6314-0

Cited by 13 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tisch et al (2002) showed the potential carcinogenicity of DZN to human nasal mucosal cells. Toxicity effects of DZN on liver, kidney, and brain were also previously reported in animal models (Ezzi et al, 2016; Tsitsimpikou et al, 2013).…”

Section: Introductionsupporting

confidence: 64%

Mechanisms of diazinon effects on impaired spermatogenesis and male infertility

et al. 2018

View full text Add to dashboard Cite

Diazinon (DZN) is an organophosphate insecticide that has cytotoxic and pathological effects on the reproductive system. It causes a wide variety of pathological effects on the reproductive system such as testicular atrophy, disturbance in sex hormones, impaired spermatogenesis, low quality of sperm, and fertility problems. However, molecular and cellular mechanisms of its adverse effects are not well understood. General events such as testicular damage, inflammation, mitochondrial deficiency, DNA fragmentation, disintegration of sperm plasma membrane, apoptosis, and cell death are observed in DZN-exposed animals. Oxidative stress (OS) induced by reactive oxygen species may be a main mechanism, which can be associated with sperm DNA fragmentation, reduced integrity of sperm cell membrane, apoptosis, depletion of antioxidants, and subsequently poor sperm quality and male infertility. Therefore, identification of these pathways may provide valuable information regarding the mechanisms of DZN action on the male reproductive system. In this review, we aim to discuss the proposed cellular and molecular mechanisms of DZN action on male reproductive system, the importance of OS and mechanisms by which DZN induces OS and depletion of other antioxidants.

show abstract

Section: Introductionsupporting

confidence: 64%

Mechanisms of diazinon effects on impaired spermatogenesis and male infertility

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Cakici et al found that Diazinon produces vacuoles in the hepatocytes, increases Kupffer and inflammatory cells, and promotes congestion of the central veins in mice 9 . Ezzi et al in their research also proved that Diazinon distorts portal triads and congestion of central veins and sinusoids 16 .…”

Section: Discussionmentioning

confidence: 89%

Evaluation of the effects of the black mulberry extract against Diazinon induced hepatotoxicity in albino wistar rats.

Javed

Kalhoro

Memon

et al. 2021

Int. j. endorsing health sci. res.

View full text Add to dashboard Cite

Background: Diazinon is the globally used organophosphorus compound to which humans are exposed through contamination of food and water. Flavonoids in Morus nigra exhibit a wide range of antioxidant, antimicrobial, anti-inflammatory, anti-cancer, anti-radiation properties and have a protective action against oxidative damage and hepatoprotective effects. The study's objective was to evaluate the effects of black mulberry (Morus nigra) extract against diazinon-induced hepatotoxicity in albino Wistar rats. Methodology: A quasi-experimental study was conducted from August 2019 to January 2020 at the Department of Pathology, Isra University, Hyderabad, Pakistan. Under standard colony conditions, thirty-six healthy male albino Wistar rats weighing between 200 and 300 grams were randomly divided equally into three groups: Group-1 (Control), Group-2 (given 60 mg/kg Diazinon daily for 4 weeks), Group-3 (administered Diazinon 60 mg/kg with 500 mg/kg of Morus nigra extract daily for 4 weeks through orogastric intubation). Hepatic tissue of all groups was observed under the light microscope. At the same time, hepatic serum markers were also analyzed. Results: A statistically significant decline in the bodyweight and rise in absolute liver weight of group-3 compared with groups-1 and group-2 (p<0.05). Co-administration of Morus nigra significantly lowered serum AST ad ALT levels. Significant histopathological derangement was observed in group-2 hepatic tissues while group-3 rats hepatic tissues had minimal changes and near-normal hepatic parenchyma. Conclusion: Morus nigra leaf extract has hepatoprotective effects against Diazinon-induced hepatotoxicity in male albino Wistar rats.

show abstract

“…22 Some antioxidant enzymes, like cytosol (SOD, PRDX, GPX) and peroxisomes (CAT), localize in separate cell compartments. 23 The mRNA levels of antioxidant enzymes GPX1, PRDX6, and SOD2 significantly increased in the DZN-exposed groups (Figure 2D), possibly serving as a protective mechanism which responds to the excessive ROS, accompanied by the increased mRNA and protein of CAT (Figure 7A). Transcriptome analysis with the aid of RNA-seq in the heatmaps of oxidative stress (Figure 2C) and ROS (Figure Collectively, the results supported that DZN exposure significantly induces oxidative stress in porcine granulosa cells.…”

Section: ■ Discussionmentioning

confidence: 97%

Cytotoxicity and DNA Damage Caused from Diazinon Exposure by Inhibiting the PI3K-AKT Pathway in Porcine Ovarian Granulosa Cells

Wang

Luo

et al. 2018

J. Agric. Food Chem.

View full text Add to dashboard Cite

Organophosphorus insecticide diazinon (DZN) is diffusely used in agriculture, home gardening, and crop peats. Much work so far has focused on the link between DZN exposure and the occurrence of neurological diseases, while little is known on the reproductive toxicological assessment on DZN exposure. This research aimed to investigate the underlying mechanisms of toxic hazards for DZN exposure on cultured porcine ovarian granulosa cells. We analyzed the oxidative stress, energy metabolism, DNA damage, apoptosis, and autophagy by using high-throughput RNA-seq, immunofluorescence, Western blotting, and real-time PCR. The combined data demonstrated that DZN exposure could cause excessive ROS and DNA damage, which induced apoptosis and autophagy by inhibiting the PI3K-AKT pathway. The down-regulated CYP19A1 protein and granulosa cell deaths increase the risk for developing premature ovarian failure and follicular atresia. In conclusion, DZN exposure has obvious reproductive toxicity by induction of granulosa cell death through pathways connected to DNA damage and oxidative stress by inhibiting the PI3K-AKT pathway.

show abstract

Toxicopathic changes and genotoxic effects in liver of rat following exposure to diazinon

Cited by 13 publications

References 37 publications

Mechanisms of diazinon effects on impaired spermatogenesis and male infertility

Mechanisms of diazinon effects on impaired spermatogenesis and male infertility

Evaluation of the effects of the black mulberry extract against Diazinon induced hepatotoxicity in albino wistar rats.

Cytotoxicity and DNA Damage Caused from Diazinon Exposure by Inhibiting the PI3K-AKT Pathway in Porcine Ovarian Granulosa Cells

Contact Info

Product

Resources

About