Objective: To determine the histo-morphometric effects of energy drink consumption in different doses on the pancreatic tissue of Wistar Albino rats. Study Design: Experimental study. Setting: Department of Pharmacology, Isra University, Hyderabad. Period: April 2021 to September 2021. Material & Methods: Thirty healthy albino Wistar rats of body weight 200±20 were included in the study. Rats were divided equally into three groups. Group I (Control), Group II (Low dose energy drink 7.5 ml/day) and Group III (High dose energy drink 15 ml/day). The body weight of all animals was measured. Blood samples were collected for the biochemical analysis including serum glucose, serum insulin level, and oxidative markers. Histo-pathological and morphometric findings were observed. Results: A statistically significant rise in body weight in groups II and III (p<0.05). Significantly increased serum glucose and declined insulin level was seen in group II and III compared with controls (p<0.05). There was a significantly lower level of oxidative markers observed in group III than in groups I and II (p<0.05). The mean pancreatic acini and islets diameter in groups II and III has decreased significantly compared with group I (p<0.05). The histo-pathological grading reveals that mild to moderate parenchymal changes in the pancreatic tissue of group II rats compared with group I. While moderate to severe changes were observed in group III compared with group I and II rats. Conclusion: Caffeinated energy drink consumption results in oxidative stress and degenerative changes in the potential to seriously alter the exocrine and endocrine pancreas' normal morphology consumption.
Background: Tuberculosis is the public health disease of Pakistan. Liver is the site of metabolism for most of the antituberculosis drugs and these agents harm the liver, resulting into elevated liver enzymes following inflammation (hepatitis). Isoniazid (INH) is often being used in experimental studies in animal models for induction of liver injury. Objective: To assess the hepatoprotective effects of the Co-enzyme Q-10 in rat model with Isoniazid induced hepatotoxicity. Methodology: A total of 50 Rats of Albino Wistar category were divided in 5 equal groups (A, B, C, D and E) randomly. Group A (control) was kept on normal diet without any intervention whereas group B (experimental negative control) was given INH 100 mg/day for induction of hepatitis. Groups C was administered an oral dose of INH as 100mg+CoQ 100mg/day. The group D rats were given INH 150+ Q-10 100mg/day similarly rats in group E were administered INH 200mg+CoQ 100mg/day. Samples of blood were obtained by scarifying rats at the end of study (1month) LFTs for each group were done, comparing different groups on ANOVA using SPSS 22 version. Results: There was significant difference in serum AST, ALT, LDH, ALP, GGT and bilirubin levels between various animal groups. The p-value was 0.00 for serum ALT, p-value was 0.000 for serum AST levels while for the difference in serum LDH p-value was 0.000, for serum ALP the p-value was 0.000, whereas p-value was 0. 000 for serum gamma GT levels and the p-value was 0.000 for serum bilirubin levels among the various animal groups that was highly significant statistically. Conclusion: Co-enzyme Q-10 improved INH induced changes in liver functions and histology.
Background: Diazinon is the globally used organophosphorus compound to which humans are exposed through contamination of food and water. Flavonoids in Morus nigra exhibit a wide range of antioxidant, antimicrobial, anti-inflammatory, anti-cancer, anti-radiation properties and have a protective action against oxidative damage and hepatoprotective effects. The study's objective was to evaluate the effects of black mulberry (Morus nigra) extract against diazinon-induced hepatotoxicity in albino Wistar rats. Methodology: A quasi-experimental study was conducted from August 2019 to January 2020 at the Department of Pathology, Isra University, Hyderabad, Pakistan. Under standard colony conditions, thirty-six healthy male albino Wistar rats weighing between 200 and 300 grams were randomly divided equally into three groups: Group-1 (Control), Group-2 (given 60 mg/kg Diazinon daily for 4 weeks), Group-3 (administered Diazinon 60 mg/kg with 500 mg/kg of Morus nigra extract daily for 4 weeks through orogastric intubation). Hepatic tissue of all groups was observed under the light microscope. At the same time, hepatic serum markers were also analyzed. Results: A statistically significant decline in the bodyweight and rise in absolute liver weight of group-3 compared with groups-1 and group-2 (p<0.05). Co-administration of Morus nigra significantly lowered serum AST ad ALT levels. Significant histopathological derangement was observed in group-2 hepatic tissues while group-3 rats hepatic tissues had minimal changes and near-normal hepatic parenchyma. Conclusion: Morus nigra leaf extract has hepatoprotective effects against Diazinon-induced hepatotoxicity in male albino Wistar rats.
The aims and objectives of current study was to check and concluded the chances of any drug interaction of metformin with hypertensive treatment with beta- blockers and calcium channel blockers in type 2 diabetic patients. During study diabetic patients with type 2 were treated with metformin and hypertensive drugs i.e. beta- blockers and calcium channel blockers. There is no any drug interaction in both gender has seen (1.00± 00.01, 1.00± 00.01). while Significant change (p<0.05) regarding glucose levels, systolic and diastolic blood pressure were seen in group B and group C as compared to the group A i.e. control group. Keyword: Drug interaction, Metformin, Beta- blockers, Calcium channel blockers
Background and Objective: Resveratrol is a poly-hydroxyphenol plant toxin that alleviates oxidative stress by increasing endogenous antioxidant levels and prevents tissue damage. The study aimed to investigate the anti-oxidative role of Resveratrol by histochemical, ultrastructural, and biochemical methods in Cisplatin-induced testicular toxicity in Wistar Albino rats. Material and Methods: Quasi-experimental study was conducted at the Department of Pharmacology, Anatomy and Postgraduate Laboratory of ISRA University Hyderabad from October 2020 to March 2021. Twenty-four male, normal adult Wistar albino rats were recruited and distributed equally into; Group-A (Control), Group-B (Experimental group or Cisplatin group), Group-C (Experimental group or Cisplatin + Resveratrol combination group). Pre and post-experimental body weight, analysis of oxidative markers, semen parameters, and histomorphology were carried out in all three groups. SPSS version 24.0 was used for the analysis of Data. Results: A statistically significant decline in the body weight and testicular weight in group B and C respectively (p<0.05). While reduction in sperm count, motility and viability was observed in Group-B compared with Group-C (p<0.05). Oxidative markers were also significantly depleted in Group-B in comparison to Group-C (p<0.05). Evident changes were observed in the testicular histology of Group-B compared with Group-C (p<0.05). Irregular, regressive, and atrophic seminiferous tubules were seen in Group-B. Most seminiferous tubules having normal morphology were observed in Group-C while the number of atrophic and degenerative seminiferous tubules also decreased significantly. Conclusion: Resveratrol is a potent protective agent with promising results in attenuating cisplatin-induced oxidative stress and eventually testicular toxicity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.