Toxicological impact of organophosphorus Chlorpyrifos 48%EC pesticide on hemocytes, biochemical disruption, and molecular changes in Biomphalaria alexandrina snails

Ibrahim, Amina M.; Hussein, Ahmed A.A.

doi:10.1016/j.pestbp.2022.105154

Cited by 14 publications

(8 citation statements)

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“…48 The protein electrophoretic pattern of B. alexandrina and B. trauncatus snails exposed to the ethanol extracts of plants, Euphorbia splendens, Ziziphus spinachristi, and Ambrosia maritime, revealed differences in the number and molecular weight of protein bands compared to control snails. 49 It was also reported that B. alexandrina snails exposed to LC10 of diazinon (1.9 ppm) and profenfos (0.75 ppm) showed changes in the number and molecular weights of protein bands compared to the control group. 50 The differences in native protein fractionation into bands between the treated and control snails could be attributed to changes in the DNA of the treated snails, 51 production of additional proteins, or disturbance in polypeptide metabolism.…”

Section: Discussionmentioning

confidence: 87%

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Biochemical, Histological Changes, Protein Electrophoretic Pattern, and Field Application of CuPb–Ferrite/TiO₂ Nanocomposites for Controlling Terrestrial Gastropod Eobania vermiculata (Müller)

Helmy

Ayyad

Ali

et al. 2023

J. Agric. Food Chem.

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Eobania vermiculata is a hazardous snail that can damage ornamental plants and cause significant harm to plant sections in Egyptian areas. Herein, the molluscicidal activity of CuPb−Ferrite/TiO 2 and TiO 2 nanoparticles (NPs) against E. vermiculata was evaluated using the poisonous bait method. LC50 values were determined using the leaf dipping and contact methods, with values of 631.23 and 1703.49 ppm for CuPb−Ferrite/TiO 2 and 193.67 and 574.97 ppm for TiO 2 . Exposure to both NPs resulted in a significant increase in the biochemical parameters of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), as well as a decrease in total protein (TP) percentage of E. vermiculata. Histological examinations revealed that many digestive cells had ruptured, and their contents had been lost, while the foot's epithelial layer became ruptured. The average reduction was 66.36% for CuPb−Ferrite/TiO 2 NPs compared to the recommended molluscicide, Neomyl, with a 70.23% reduction in the field application. Electrophoretic separation of total protein using sodium dodecyl sulfatepolyacrylamide gel electrophoresis after treatment with LC50 concentrations of TiO 2 and CuPb−Ferrite/TiO 2 demonstrated the potency of these synthetic compounds as molluscicidal agents. Therefore, we recommend the use of CuPb−Ferrite/TiO 2 NPs as a novel land snail molluscicide because it is safe to use, and the baits are arranged to not affect irrigation water, with a high molluscicidal effect.

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Section: Discussionmentioning

confidence: 87%

“…alexandrina and B. trauncatus snails exposed to the ethanol extracts of plants, Euphorbia splendens, Ziziphus spinachristi, and Ambrosia maritime, revealed differences in the number and molecular weight of protein bands compared to control snails . It was also reported that B.…”

Section: Discussionmentioning

confidence: 93%

Biochemical, Histological Changes, Protein Electrophoretic Pattern, and Field Application of CuPb–Ferrite/TiO₂ Nanocomposites for Controlling Terrestrial Gastropod Eobania vermiculata (Müller)

Helmy

Ayyad

Ali

et al. 2023

J. Agric. Food Chem.

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“…Hormonal reductions observed in Bulinus truncatus and increases in Biomphalaria alexandrina may contribute to fecundity loss in these infected snails [ 89 ]. Steroid hormones play an important role in gonad development in snails [ 90 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of Schistosoma sp., Infection on Biological, Feeding, Physiological, Histological, and Genotoxicological Aspects of Biomphalaria alexandrina and Bulinus truncatus Snails

Dokmak,

Hammam,

Ibrahim

2024

Acta Parasit.

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Background Trematode infections of the genus Schistosoma can induce physiological and behavioral changes in intermediate snail hosts. This is because the parasite consumes essential resources necessary for the host's survival, prompting hosts to adapt their behavior to maintain some level of fitness before parasite-induced mortality occurs. Methods In this study, the reproductive and biochemical parameters of Biomphalaria alexandrina and Bulinus truncatus were examined during the cercareal shedding stage of infection with Schistosoma mansoni and Schistosoma haematobium, respectively, compared with controls. Results The study revealed an infection rate of 34.7% for S. mansoni and 30.4% for S. haematobium. In B. alexandrina infected with S. mansoni, a survival rate of 65.2% was recorded, along with a mean prepatent period of 30.3 ± 1.41 days, a mean shedding duration of 14.2 ± 0.16 days, and a mean lifespan of 44.1 ± 0.24 days. Meanwhile, in B. truncatus infected with S. haematobium, a survival rate of 56.4% was observed, with a mean prepatent period of 44.3 ± 1.41 days, a mean shedding duration of 22.6 ± 2.7 days, and a mean lifespan of 66.9 ± 1.6 days. Feeding increased in both infected species of snails, while the net reproductive rate (Ro) of the infected snails decreased. Total antioxidant (TAO) and lipid peroxidation activity increased in the two infected snail species during shedding, while Glutathione-S-transferase levels decreased. Lipid peroxidase activity and nitrogen oxide levels significantly decreased in infected B. alexandrina and increased in infected Bulinus. Steroid hormone levels were elevated in infected Biomphalaria, whereas they were reduced in infected Bulinus. Comet assay parameters showed an increase in the two infected genera after infection compared to control snails, indicating genotoxic damage and histopathological damage was observed. Conclusions These findings demonstrate that infection with larva species diverse biochemical, hormonal, genotoxic, and histopathological changes in the tissues responsible for fecundity and reproduction in B. alexandrina and B. truncates comparing with controls.

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“…Steroid hormones have been reported in numerous molluscs, including B. alexandrina(Oehlmann and Schulte-Oehlmann, 2003;Croll and Wang, 2007;Omran, 2012;Ragheb et al, 2018)d truncatus(Dokmak et al, 2022). Hormonal reduction observed in Bulinus truncatus and increased in Biomphalaria alexandrina may contribute to fecundity loss in these infected snails(Ibrahim and Hussein, 2022). Steroid hormones are important for gonad development in snails(Alon et al, 2007).The study found that infection with cercariae mansoni and haematobium caused a statistically signi cant increase in DNA fragmentation and migration in molluscan tissues compared to controls.…”

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confidence: 82%

Impact of Schistosoma sp., infection on biological, behavioral, physiological, histological, and genotoxicological aspects of Biomphalaria alexandrina and Bulinus truncatus snails

Dokmak,

hamam,

ibrahim

2023

Preprint

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Trematodes infection of genus Schistosoma can lead to physiological and behavioral changes in intermediate snail hosts. This is because the parasite consumes essential resources required for the host's survival, but the hosts can adaptively modify their behavior to ensure some level of fitness before parasite-induced mortality. The present study examined the reproductive and biochemical parameters of Biomphalaria alexandrina and Bulinus truncatus during the shedding stage of infection with Schistosoma mansoni and haematobium, respectively. The study found that the infection rate with S. mansoni was 34.7% and 30.4% with S. haematobium. In B.alexandrina infected with S. mansoni, a survival rate of 65.2% was recorded, along with a mean duration of shedding of 32.8 ± 5.5 days, a mean prepatent period of 37.5 ± 1.15 days, and a mean life span of 57.0 ± 1.21 days. Meanwhile, in B. truncatus infected with S. haematobium, a survival rate of 56.4% was recorded, with a mean duration of shedding of 42.6 ± 2.6 days, a mean prepatent period of 46.7 ± 2.3 days, and a mean life span of 65.9 ± 1.6 days. The feeding behavior was increased in the two infected species snail, while the net reproductive rate (Ro) of the infected species snails was reduced. Total antioxidant (TAO) and lipid peroxidation activity were increased in the two infected snails during shedding, while Glutathione-S-transferase was reduced. Lipid peroxidase (LPO) activity and nitrogen oxide (NO) levels decreased significantly in infected B. alexandrina and increased in infected Bulinus. Steroid hormone measurements were increased in the infected Biomphalaria, while they were reduced in infected Bulinus. Comet assay parameters were increased in the two infected genera after infection than control snails, and histopathological damage occurred. These observations demonstrated that infection initiates diverse biochemical, hormonal, genotoxic, and histopathological change to the tissues responsible for fecundity and reproduction in B. alexandrina and B. truncatus.

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Toxicological impact of organophosphorus Chlorpyrifos 48%EC pesticide on hemocytes, biochemical disruption, and molecular changes in Biomphalaria alexandrina snails

Cited by 14 publications

References 49 publications

Biochemical, Histological Changes, Protein Electrophoretic Pattern, and Field Application of CuPb–Ferrite/TiO₂ Nanocomposites for Controlling Terrestrial Gastropod Eobania vermiculata (Müller)

Biochemical, Histological Changes, Protein Electrophoretic Pattern, and Field Application of CuPb–Ferrite/TiO₂ Nanocomposites for Controlling Terrestrial Gastropod Eobania vermiculata (Müller)

Impact of Schistosoma sp., Infection on Biological, Feeding, Physiological, Histological, and Genotoxicological Aspects of Biomphalaria alexandrina and Bulinus truncatus Snails

Impact of Schistosoma sp., infection on biological, behavioral, physiological, histological, and genotoxicological aspects of Biomphalaria alexandrina and Bulinus truncatus snails

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Toxicological impact of organophosphorus Chlorpyrifos 48%EC pesticide on hemocytes, biochemical disruption, and molecular changes in Biomphalaria alexandrina snails

Cited by 14 publications

References 49 publications

Biochemical, Histological Changes, Protein Electrophoretic Pattern, and Field Application of CuPb–Ferrite/TiO2 Nanocomposites for Controlling Terrestrial Gastropod Eobania vermiculata (Müller)

Biochemical, Histological Changes, Protein Electrophoretic Pattern, and Field Application of CuPb–Ferrite/TiO2 Nanocomposites for Controlling Terrestrial Gastropod Eobania vermiculata (Müller)

Impact of Schistosoma sp., Infection on Biological, Feeding, Physiological, Histological, and Genotoxicological Aspects of Biomphalaria alexandrina and Bulinus truncatus Snails

Impact of Schistosoma sp., infection on biological, behavioral, physiological, histological, and genotoxicological aspects of Biomphalaria alexandrina and Bulinus truncatus snails

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Biochemical, Histological Changes, Protein Electrophoretic Pattern, and Field Application of CuPb–Ferrite/TiO₂ Nanocomposites for Controlling Terrestrial Gastropod Eobania vermiculata (Müller)

Biochemical, Histological Changes, Protein Electrophoretic Pattern, and Field Application of CuPb–Ferrite/TiO₂ Nanocomposites for Controlling Terrestrial Gastropod Eobania vermiculata (Müller)