Toxicological effects of acrylamide on rat testicular gene expression profile

Yang, Hyejin; Lee, Sang‐Hyun; Jin, Yong; Choi, Jinhyang; Han, Dong-Un; Chae, Chanhee; Lee, Mun-Han; Han, Chang-Hoon

doi:10.1016/j.reprotox.2004.10.006

Cited by 74 publications

(69 citation statements)

References 36 publications

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“…Furthermore, Yang et al (6) proved that level of testosterone hormone in rats was severely affected in different doses of acrylamide and this led to the spermatogenic effects and decreased the Leydig cell viability. Also, Hamdy et al (7) indicated that testis is a target organ of acrylamide action as it caused severe damage in seminiferous tubules and caused decrease in plasma free and total testosterone in a dose dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Effects of Lipoic Acid on Acrylamide Induced Testicular Damage

2014

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Introduction:Acrylamide is very toxic to various organs and associated with significant increase of oxidative stress and depletion of antioxidants. Alpha-lipoic acid enhances cellular antioxidant defense capacity, thereby protecting cells from oxidative stress.Aim of the study:This study aimed to evaluate the protective role of alpha-lipoic acid on the oxidative damage induced by acrylamide in testicular and epididymal tissues.Material and methods:Forty adult male rats were divided into four groups (10 rats each). Control group; acrylamide treated group administered acrylamide 0.05% (w/v) in drinking water for 21 days; alpha-lipoic acid group received basal diet supplemented with 1% alpha-lipoic acid and forth group was exposed to acrylamide and treated with alpha-lipoic acid at the same doses and treatment regimen mentioned before.Results:The administration of acrylamide resulted in significant elevation in testicular and epididymal malondialdehyde level (MDA) and significant reduction in the level of reduced glutathione (GSH) and the activities of glutathione-S-transferase (GST), glutathione peroxidase (GPX) and glutathione reductase (GR). Also, acrylamide significantly reduced serum total testosterone and progesterone but increased estradiol (E2) levels. Treatment with alpha-lipoic acid prior to acrylamide induced protective effects and attenuated these biochemical changes.Conclusion:Alpha-lipoic acid has been shown to possess antioxidant properties offering promising efficacy against oxidative stress induced by acrylamide administration.

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Section: Discussionmentioning

confidence: 99%

Effects of Lipoic Acid on Acrylamide Induced Testicular Damage

2014

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“…ACR-induced histopathological lesions, such as formation of multinucleated giant cells and vacuolation, and numerous apoptotic cells were observed in seminiferous tubules. Serum testosterone level and Leydig cell viability were also decreased dose-dependently, which resulted in decreased spermatogenesis (Yang et al 2005). Although not active in the in vitro Ames test, ACR does elicit mutagenic effects in stem cell spermatogonia (Dearfield et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis induced by acrylamide is suppressed in a 21.5% fat diet through caspase-3-independent pathway in mice testis

Zhang

Chen

Huang

2009

Toxicology Mechanisms and Methods

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This study investigates the simultaneous effect of acrylamide (ACR) and high-fat-intake on the apoptosis in testis cells, and also the expression and activity of caspase-3. Seventy-two male Kunming mice were divided into two blocks and fed with a high-fat diet (crude fat 21.5%) or basic diet (crude fat 4.4%), respectively; and animals in each diet block were exposed to ACR at the dose of 20 mg/kgbw•d or 40 mg/kgbw•d as ACR treated groups or the normal saline as control. Germ cells prepared from testis were stained with Hoechst dye 33258 and paraffin wax sections from testis were suffered to a TUNEL process. Expression of caspase-3 on protein level was investigated using an immunohistochemical analysis assay. The supernatant of unilateral testes were subjected to a Caspase-3 activity kit to determine the activity of Caspase-3 in testis. The concentration of ACR and glycidamide(GA), epox-ide of ACR, in plasma and testis were detected by LC-ES/MS/MS analysis. Results based on the morphological changes, percentage of apoptotic cells, and integrated optical density (IOD) of positive amethyst staining which indicates the apoptotic DNA fragmentation, show that apoptosis was induced by acrylamide only; however, acr-ylamide-induced apoptosis was weakened by high-fat-intake. The protein expression and activity of Caspase-3 were not induced by ACR or high-fat-intake. Moreover, no significant differences of ACR and GA concentration were found between the high-fat and basic diet groups after exposure of ACR. Results indicate that high-fat-intake reverses the effects on apoptosis induced by ACR; and more possibly, apoptosis is induced by a caspase-3-independent mechanism.

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“…To assess the genotoxicity, five animals from each treatment were killed after 2 weeks and another five animals within each treatment were killed after 8 weeks. The dose of Direct Violet (1000 ppm kg −1 day −1 ) was chosen according to NTPTRS (1994), while the dose of acrylamide (30 mg kg −1 day −1 ) was chosen according to Yang et al (2005).…”

Section: Animalsmentioning

confidence: 99%

Genotoxicity studies on the removal of a direct textile dye by a fungal strain, in vivo, using micronucleus and RAPD‐PCR techniques on male rats

El‐Rahim

Khalil

Eshak

2007

J of Applied Toxicology

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The genotoxicity of the azo dye 'Direct Violet' and the removal of this dye by Aspergillus niger strain at different conditions have been investigated in male rats. Two genotoxicity techniques, namely bone marrow micronucleus assay and RAPD fingerprinting pattern, were used in this study for the direct dye and its removal by the fungal strain. Sixty male rats were divided into six treatment groups including a control group and other groups which were exposed for 2 or 8 weeks to Direct Violet dye, Direct Violet dye treated with A. niger at pH 2 or pH 9 or without agitation and acrylamide (30 mg/kg b.w.). A potent dose-dependent response was observed following oral gavage of the dye up to 1000 mg kg(-1), after which significant toxicity to the erythroid compartment was observed. Acrylamide and Direct Violet treatments increased the number of micronucleated polychromatic erythrocytes (MnPCEs) with respect to the controls. This increase was statistically significant in the two time intervals (2 and 8 weeks treatment, P < 0.0001). Fungi treatments at pH 2 and without agitation were able to reduce the number of MnPCEs induced by Direct Violet administration in all duration groups. Fungi treatment at pH 9 was only able to inhibit the genotoxicity of Direct Violet after 8 weeks treatment. The RAPD fingerprinting pattern indicated that most DNA of the samples treated with dye alone or acrylamide revealed polymorphic bands including the appearance and disappearance of the bands, which did not appear in the DNA samples of normal or fungi protected rats. The implications of these findings for the health and safety of occupationally exposed workers are discussed.

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Toxicological effects of acrylamide on rat testicular gene expression profile

Cited by 74 publications

References 36 publications

Effects of Lipoic Acid on Acrylamide Induced Testicular Damage

Effects of Lipoic Acid on Acrylamide Induced Testicular Damage

Apoptosis induced by acrylamide is suppressed in a 21.5% fat diet through caspase-3-independent pathway in mice testis

Genotoxicity studies on the removal of a direct textile dye by a fungal strain, in vivo, using micronucleus and RAPD‐PCR techniques on male rats

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